Results 61 to 70 of about 1,731,641 (260)

Analysis of the paired TCR α- and β-chains of single human T cells. [PDF]

, 2012
Analysis of the paired i.e. matching TCR α- and β-chain rearrangements of single human T cells is required for a precise investigation of clonal diversity, tissue distribution and specificity of protective and pathologic T-cell mediated immune responses.
Dornmair, K., Besgen, P., Held, K., Bhonsle, L., Vollmer, Sigrid, Song-Min Kim, Jens Nickel, Anna Backes, Held, Kathrin, Vollmer, S., Joerg C Prinz, Backes Anna, Backes, A., Backes, Anna, Bhonsle, Latika, Kim Song-Min, Nickel, Jens, Sigrid Vollmer, Nickel, J., Kim, Song-Min, Klaus Dornmair, Dornmair, Klaus, Kim, S., Besgen Petra, Petra Besgen, Vollmer Sigrid, Prinz, Joerg C., Prinz Joerg C., Latika Bhonsle, Bhonsle Latika, Besgen, Petra, Held Kathrin, Prinz, J., Kathrin Held, Nickel Jens, Dornmair Klaus   +35 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

From silencing to gene expression: real-time analysis in single cells.

Cell, 2004
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into a euchromatic region of chromosome 1 in human U2OS cells. The condensed array is heterochromatic as it is associated with HP1, histone H3 methylated at lysine 9 ...
Janicki, Susan M, Tsukamoto, Toshiro, Salghetti, Simone E, Tansey, William P, Sachidanandam, Ravi, Prasanth, Kannanganattu V, Ried, Thomas, Shav-Tal, Yaron, Bertrand, Edouard, Singer, Robert H, Spector, David L   +10 more
openaire   +2 more sources

Microgenomics: gene expression analysis at the tissue-specific and single-cell levels [PDF]

Journal of Experimental Botany, 2005
Plant organs are composed of many different cell types and the analysis of 'bulk' material results in the average of all information in these cells. Therefore, this does not reflect any individuality of the tissues present in plants. This review briefly summarizes different sampling methods which provide tissue- and cell-specific samples, respectively.
openaire   +2 more sources

Towards a digital model of zebrafish embryogenesis. Integration of Cell Tracking and Gene Expression Quantification

, 2010
We elaborate on a general framework composed of a set of computational tools to accurately quantificate cellular position and gene expression levels throughout early zebrafish embryogenesis captured over a time-lapse series of in vivo 3D images.
Melani, Camilo, Santos Lleo, Andres de, Bourgine, Paul, Douloquin, Louise, Desnoulez, Sophie, Savy, Thierry, Castro González, Carlos, Luengo Oroz, Miguel Ángel, Peyrieras, Nadine, Ledesma Carbayo, María Jesús   +9 more
core  

Ensemble machine learning on gene expression data for cancer classification [PDF]

, 2003
Whole genome RNA expression studies permit systematic approaches to understanding the correlation between gene expression profiles to disease states or different developmental stages of a cell.
Tan, A C, Gilbert, D
core  

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Stochastic neural network models for gene regulatory networks [PDF]

, 2003
Recent advances in gene-expression profiling technologies provide large amounts of gene expression data. This raises the possibility for a functional understanding of genome dynamics by means of mathematical modelling.
Burrage K., Tian, T., Tian T., Burrage, K.   +3 more
core   +1 more source