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2019
Active DNA demethylation plays important roles in the epigenetic reprogramming of developmental processes. 5-formylcytosine (5fC) is produced during active demethylation of 5-methylcytosine (5mC). Here, we describe a technique called CLEVER-seq (Chemical-labeling-enabled C-to-T conversion sequencing), which detects the whole genome 5fC distribution at ...
Chenxu, Zhu +4 more
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Active DNA demethylation plays important roles in the epigenetic reprogramming of developmental processes. 5-formylcytosine (5fC) is produced during active demethylation of 5-methylcytosine (5mC). Here, we describe a technique called CLEVER-seq (Chemical-labeling-enabled C-to-T conversion sequencing), which detects the whole genome 5fC distribution at ...
Chenxu, Zhu +4 more
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Single cell epigenome sequencing technologies
Molecular Aspects of Medicine, 2018Epigenetic regulation plays crucial roles in the development and disease processes. Since different cell types with distinct epigenetic characteristics are always intermingled together in the tissues or organs, the single cell analysis provides a universal resolution for dissecting their intrinsic complexities.
Lu, Wen, Fuchou, Tang
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Single Cell RNA Sequencing in NASH
2022Single cell RNA sequencing (scRNA-seq) allows to uncover cellular heterogeneity and the identification of novel subpopulations. In non-alcoholic steatohepatitis (NASH), scRNA-seq is particularly powerful to understand non-parenchymal cell heterogeneity in the liver, e.g. for inflammatory cells.
Jana Hundertmark +2 more
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Introduction to Single‐Cell RNA Sequencing
Current Protocols in Molecular Biology, 2018AbstractDuring the last decade, high‐throughput sequencing methods have revolutionized the entire field of biology. The opportunity to study entire transcriptomes in great detail using RNA sequencing (RNA‐seq) has fueled many important discoveries and is now a routine method in biomedical research. However, RNA‐seq is typically performed in “bulk,” and
Thale Kristin, Olsen, Ninib, Baryawno
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Single-cell sequencing in cancer research
Expert Review of Molecular Diagnostics, 2015Genome-wide single-cell sequencing investigations have the potential to classify individual cells within a tumor mass. In recent years, various single-cell DNA and RNA quantification techniques have facilitated significant advances in our ability to classify subpopulations of cells within a heterogeneous population.
Mireia, Mato Prado +3 more
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Application of Single Cell Sequencing in Cancer
2018Cancer is a heterogenetic disease at both the level of clinical manifestation and the level of the genome. Single-cell sequencing provides an unprecedented means of characterizing the intra-tumor heterogeneity and detecting and analyzing the genomes of cancer cells.
Lan, Yu +3 more
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Application of Single-Cell Sequencing to Immunotherapy
Urologic Clinics of North America, 2020Cancer is a highly complex and heterogeneous disease and immunotherapy has shown promise as a therapeutic approach. The increased resolution afforded by single-cell analysis offers the hope of finding and characterizing previously underappreciated populations of cells that could prove useful in understanding cancer progression and treatment.
Kristin G, Beaumont +2 more
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Applications of Single-Cell Sequencing for Multiomics
2018Single-cell sequencing interrogates the sequence or chromatin information from individual cells with advanced next-generation sequencing technologies. It provides a higher resolution of cellular differences and a better understanding of the underlying genetic and epigenetic mechanisms of an individual cell in the context of its survival and adaptation ...
Yungang, Xu, Xiaobo, Zhou
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Single-Cell Sequencing in Genitourinary Malignancies
2020Single-cell sequencing (SCS) is a powerful new tool that applies Next Generation Sequencing at the cellular level. SCS has revolutionized our understanding of tumor heterogeneity and the tumor microenvironment, immune infiltration, cancer stem cells (CSCs), circulating tumor cells (CTCs), and clonal evolution.
Neal, Murphy +6 more
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Single Cell Sequencing in Cancer Diagnostics
2020Personalized medicine has been driven by improvements in genomic sequencing and analysis. For several diseases, in particular cancers, it has for nearly a decade been standard clinical practice to analyze the genome and expression of the genes of patients.
Frederik Otzen, Bagger, Victoria, Probst
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