Results 71 to 80 of about 1,166,241 (315)
SMARTer single cell total RNA sequencing [PDF]
Nucleic Acids Research, 2019 Abstract Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these methods suffer from one or more limitations, such as focusing only on polyadenylated RNA, sequencing of only the 3′ end of the transcript, an exuberant fraction of reads mapping to ribosomal RNA ...
Karen Verboom +12 more
openaire +3 more sources
Design and Analysis of Single-Cell Sequencing Experiments
, 2015 Recent advances in single-cell sequencing hold great potential for exploring biological systems with unprecedented resolution. Sequencing the genome of individual cells can reveal somatic mutations and allows the investigation of clonal dynamics.
Grün, D. +3 more
core +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
Lu scRNA-seq Mouse Fetal Liver 2021
, 2023 Dataset published by Lu et al. 2021, which contains single-cell RNA sequencing data of E14.5 whole fetal liver cells. Citation Lu, Y., Liu, M., Yang, J. et al. Spatial transcriptome profiling by MERFISH reveals fetal liver hematopoietic stem cell niche
Single-cell best practices (14656733)
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FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Mesothelioma cell heterogeneity identified by single cell RNA sequencing
Scientific ReportsMesothelioma cell heterogeneity encompasses diverse morphological and molecular characteristics observed within tumors, significantly impacting disease progression, treatment outcomes, and the development of targeted therapies.
Licun Wu +4 more
doaj +1 more source
Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics
STAR ProtocolsSummary: The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for ...
Yan Ma +4 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Applications of single-cell sequencing in cancer research: progress and perspectives
Journal of Hematology & Oncology, 2021 Single-cell sequencing, including genomics, transcriptomics, epigenomics, proteomics and metabolomics sequencing, is a powerful tool to decipher the cellular and molecular landscape at a single-cell resolution, unlike bulk sequencing, which provides ...
Yalan Lei +7 more
doaj +1 more source
Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing
, 2020 The libraries generated by high-throughput single cell RNA-sequencing (scRNA-seq) platforms such as the Chromium from 10x Genomics require considerable amounts of sequencing, typically due to the large number of cells.
Senabouth, A (15675581) +14 more
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