Results 61 to 70 of about 1,166,241 (315)
Journal of Experimental & Clinical Cancer Research, 2021 Breast cancer is a heterogeneous disease with a complex microenvironment consisting of tumor cells, immune cells, fibroblasts and vascular cells. These cancer-associated cells shape the tumor microenvironment (TME) and influence the progression of breast
Xueqi Yan +12 more
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Recent advances in single-cell epigenetic sequencing technology
Di-san junyi daxue xuebao, 2022 Single-cell sequencing technologies are important hot tools for decoding the heterogeneity of tissues, organs, cells and molecules, mainly based on the next-generation sequencing technology.
NI Bing, GAO Weiwu
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Single-cell isolation by a modular single-cell pipette for RNA-sequencing [PDF]
Lab on a Chip, 2016 We report a modular single-cell pipette (mSCP), consisting of an air-displacement pipette (ADP), ADP-Tips, and a SCP-Tip, and its application in single-cell isolation for RNA-sequencing.
Kai Zhang +6 more
openaire +2 more sources
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Massively parallel nanowell-based single-cell gene expression profiling
BMC Genomics, 2017 Background Technological advances have enabled transcriptome characterization of cell types at the single-cell level providing new biological insights.
Leonard D. Goldstein +23 more
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FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
Single Cell T Cell Receptor Sequencing: Techniques and Future Challenges
Frontiers in Immunology, 2018 The peculiarity of T cell is their ability to recognize an infinite range of self and foreign antigens. This ability is achieved during thymic development through a complex molecular mechanism based on somatic recombination that leads to the expression ...
Marco De Simone +3 more
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Single-cell RNA-sequencing in asthma research
Frontiers in Immunology, 2022 Asthma is a complex and heterogeneous disease with multicellular involvement, and knowledge gaps remain in our understanding of the pathogenesis of asthma. Efforts are still being made to investigate the immune pathogenesis of asthma in order to identify
Weifeng Tang +11 more
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Single-cell RNA sequencing of human kidney
, 2019 A comprehensive cellular anatomy of normal human kidney is crucial to address the cellular origins of renal disease and renal cancer. Some kidney diseases may be cell type-specific, especially renal tubular cells.
Zhenyuan Yu (6698111) +2 more
core +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source