Results 41 to 50 of about 1,166,241 (315)

Single-cell profiling of transcriptome and histone modifications with EpiDamID [PDF]

, 2022
Recent advances in single-cell sequencing technologies have enabled simultaneous measurement of multiple cellular modalities, but the combined detection of histone post-translational modifications and transcription at single-cell resolution has remained ...
Rang, Franka J, Sato, Yuko, Nguyen, Phong D, de Luca, Kim L, Rang, Franka J., de Vries, Sandra S, Bakkers, Jeroen, Nguyen, Phong D., de Vries, Sandra S., de Luca, Kim L., Kind, Jop, Boele, Ellen, Guerreiro, Isabel, Valdes-Quezada, Christian, Kimura, Hiroshi   +14 more
core   +1 more source

The Application of Single‐Cell RNA Sequencing in Vaccinology [PDF]

Journal of Immunology Research, 2020
Single‐cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited‐volume samples, advancing prospects of a new era of systems immunology. The power of single‐cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines.
Noé, A, Cargill, TN, Nielsen, CM, Russell, AJC, Barnes, E   +4 more
openaire   +4 more sources

CRISPR screening in single cells

, 2019
The combination of single-cell RNA-seq and CRISPR allows for efficient interrogation of possibly any number of genes, only limited by the sequencing capability. Here we describe the current protocols for CRISPR screening in single cells, from cloning and
Henriksson, Johan,
core   +1 more source

An information-theoretic approach to single cell sequencing analysis

BMC Bioinformatics, 2023
Background Single-cell sequencing (sc-Seq) experiments are producing increasingly large data sets. However, large data sets do not necessarily contain large amounts of information. Results Here, we formally quantify the information obtained from a sc-Seq
Michael J. Casey, Jörg Fliege, Rubén J. Sánchez-García, Ben D. MacArthur   +3 more
doaj   +1 more source

Single‐cell RNA sequencing in osteoarthritis

Cell Proliferation, 2023
AbstractOsteoarthritis is a progressive and heterogeneous joint disease with complex pathogenesis. The various phenotypes associated with each patient suggest that better subgrouping of tissues associated with genotypes in different phases of osteoarthritis may provide new insights into the onset and progression of the disease.
Yuyuan Gu, Yan Hu, Hao Zhang, Sicheng Wang, Ke Xu, Jiacan Su   +5 more
openaire   +2 more sources

Having a direct look:analysis of DNA damage and repair mechanisms by next generation sequencing [PDF]

, 2014
Genetic information is under constant attack from endogenous and exogenous sources, and the use of model organisms has provided important frameworks to understand how genome stability is maintained and how various DNA lesions are repaired. The advance of
Gartner, Anton, Meier, Bettina
core   +1 more source

Single-Cell RNA Sequencing: Opportunities and Challenges for Studies on Corneal Biology in Health and Disease

, 2023
The structure and major cell types of the multi-layer human cornea have been extensively studied. However, various cell states in specific cell types and key genes that define the cell states are not fully understood, hindering our comprehension of ...
Dulce Lima Cunha, Camille Laberthonnière, Julian A. Arts, Huiqing Zhou   +3 more
core   +1 more source

European Standard Clinical Practice Guideline and EXPeRT Recommendations for the Diagnosis and Management of Gastroenteropancreatic Neuroendocrine Neoplasms in Children and Adolescents

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Pediatric gastroenteropancreatic neuroendocrine neoplasms (GEP‐NENs) are extremely rare and clinically heterogeneous. Management has largely been extrapolated from adult practice. This European Standard Clinical Practice Guideline (ESCP), developed by the EXPeRT network in collaboration with adult NEN experts, provides (adult) evidence ...
Michaela Kuhlen, Calogero Virgone, Antje Redlich, Michael Abele, Ricardo Lopez‐Almaraz, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Maja Mazic Cesen, Andrea Ferrari, Malgorzata A. Krawczyk, Yves Reguerre, Dominik T. Schneider, Ines B. Brecht, Charlotte Rigaud, Bilgehan Yalcin, Rainer Claus, Christian Vokuhl, Constantin Lapa, Jörg Fuchs, Segolene Hescot, Thomas Walter, Julien Hadoux, Daniel Orbach   +23 more
wiley   +1 more source

Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing [PDF]

, 2017
Summary: The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation.
Emmanouil T. Dermitzakis, Serge Nef, Antonarakis, Stylianos E., Neirijnck, Yasmine, Nef, S, Yasmine Neirjinck, Isabelle Stévant, Neirijnck, Y, Jessica Escoffier, Stévant, I, Borel, Christelle, Dermitzakis, ET, Stévant, Isabelle, Nef, Serge, Smith, Lee B., Dermitzakis, Emmanouil T, Lee B. Smith, Escoffier Breancon, Jessica, Borel, C, Antonarakis, Stylianos, Stylianos E. Antonarakis, Antonarakis, SE, Dermitzakis, Emmanouil, Escoffier, J, Smith, LB, Christelle Borel, Smith, Lee B, Escoffier, Jessica, Yasmine Neirijnck   +28 more
core   +1 more source

Deep Sequencing of FLT3‐ITD Enables Response Evaluation and Post‐Treatment Monitoring in Childhood AML: An Exploratory Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Background An internal tandem duplication in the gene encoding Fms‐like tyrosine kinase 3 (FLT3‐ITD) is associated with high relapse risk and poor prognosis in acute myeloid leukemia (AML) and plays a crucial role in treatment decisions. Measurable residual disease (MRD) analysis of FLT3‐ITD during and after treatment has shown prognostic ...
Sofie Johansson Alm, Bea Tornberg, Erik Delsing Malmberg, Henrik Hasle, Kristian Løvvik Juul‐Dam, Lars Palmqvist, Borhan R. Saeed, Giti Shah Barkhordar, Anastasia Soboli, Anna Staffas, Jonas Abrahamsson, Linda Fogelstrand   +11 more
wiley   +1 more source