Results 81 to 90 of about 1,166,241 (315)

Clustering of single-cell RNA sequencing datasets with the hierarchical Dirichlet process [PDF]

Single-cell sequencing is a powerful technology that allows researchers to understand and analyse genetic and genomic information of individual cells within a heterogeneous population. This thesis focuses on two types of single-cell data, namely, 1) high-
Liu, Jinlu
core   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Stephenson Covid-19 PBMC 2021 TCR

, 2023
Dataset published by Stephenson et al. 2021, which contains transcriptome sequencing for over 750,000 Peripheral Blood Mononuclear Cells (PBMC) from 130 patients in the context of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) The dataset ...
Single-cell best practices (14656733)
core   +1 more source

Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing [PDF]

, 2020
This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow ...
Jaeger, Baptiste N., Kruse, Merit, Jaeger, Baptiste N, Yángüez, Emilio, Karayannis, Theofanis, Denoth-Lippuner, Annina, Jessberger, Sebastian, Karayannis, Theofanis; https://orcid.org/, Gesuita, Lorenzo   +8 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Multi-omics single-cell analysis reveals key regulators of HIV-1 persistence and aberrant host immune responses in early infection

eLife
The clearance of human immunodeficiency virus-1 (HIV-1) remains a significant public health challenge due to impaired cellular immune responses and HIV-1 maintenance during acute infection.
Dayeon Lee, Sin Young Choi, So-I Shin, Hyunsu An, Byeong-Sun Choi, Jihwan Park   +5 more
doaj   +1 more source

Stephenson Covid-19 PBMC 2021 BCR

, 2023
Dataset published by Stephenson et al. 2021, which contains transcriptome sequencing for over 750,000 Peripheral Blood Mononuclear Cells (PBMC) from 130 patients in the context of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The dataset
Single-cell best practices (14656733)
core   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Single-Cell RNA Sequencing Reveals the Immune Cell Profiling in IMQ Induced Psoriasis-Like Model

Journal of Inflammation Research, 2022
Shasha Jin,* Yixin Wang,* Chenxin Qie, Lu Yang, Yinhao Wu, Tingting Zhang, Jianwen Di, Jun Liu New Drug Screening Center, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China*These
Jin S, Wang Y, Qie C, Yang L, Wu Y, Zhang T, Di J, Liu J   +7 more
doaj  

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source