Results 211 to 220 of about 502,506 (259)

The production and application of single-chain antibody fragments

Folia Microbiologica, 2003
This review discusses methods for the single-chain antibody fragment ($cFv) generation and scFv expression systems, and describes potential applications of scFv in the therapy of viral diseases and cancer, with emphasis on intracellularly expressed scFvs (intrabodies), application of scFvs in detection and diagnostics, and their use in proteomics.
D, Blazek, V, Celer
openaire   +2 more sources

Bispecific and bifunctional single chain recombinant antibodies

Biomolecular Engineering, 2001
Bispecific and bifunctional monoclonal antibodies as second generation monoclonals, produced by conventional chemical or somatic methods, have proved useful in the immunodiagnosis and immunotherapy of cancer and other diseases. Recombinant antibodies produced by genetic engineering techniques have also become available for use in preclinical and ...
J, Kriangkum, B, Xu, L P, Nagata, R E, Fulton, M R, Suresh   +4 more
openaire   +2 more sources

Engineering of single chain antibodies for solubility

International Immunopharmacology, 2018
In the current study, we introduce a method to design intrinsically soluble single chain antibodies (scFvs) that can be easily produced in bacterial expression systems in a soluble form. Summarily, CDR loops are grafted on framework regions derived from intrinsically soluble subclass 3 (VH3 and VL3) human germline sequences.
openaire   +2 more sources

Single-Chain Antibodies as New Antithrombotic Drugs

Seminars in Thrombosis and Hemostasis, 2007
Antibodies are the most rapidly growing class of human therapeutics and the second largest class of drugs after vaccines. At present, several antibodies are approved for therapeutic use in diverse clinical settings, including oncology, chronic inflammatory diseases, transplantation, infectious diseases, and cardiovascular medicine.
Hagemeyer, Christoph Eugen, Schwarz, Meike, Peter, Karlheinz   +2 more
openaire   +3 more sources

Immobilization strategies for single‐chain antibody microarrays

PROTEOMICS, 2008
AbstractSandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust‐affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single‐chain antibodies (scFv) are affinity reagents that have greater potential for high‐throughput production than traditional ...
Shannon L, Seurynck-Servoss, Cheryl L, Baird, Keith D, Miller, Noah B, Pefaur, Rachel M, Gonzalez, David O, Apiyo, Heather E, Engelmann, Sudhir, Srivastava, Jacob, Kagan, Karin D, Rodland, Richard C, Zangar   +10 more
openaire   +2 more sources

Reducing heterophilic antibody interference in immunoassays using single-chain antibodies

Analytical Biochemistry, 2011
Sandwich enzyme-linked immunosorbent assay (ELISA) microarrays can simultaneously quantify the levels of multiple diagnostic targets in a biological sample. However, as with traditional ELISA diagnostics, endogenous antibodies in patient sera can cause interference.
Cheryl L, Baird, Ruimin, Tan, Christopher J, Fischer, Kristin D, Victry, Richard C, Zangar, Karin D, Rodland   +5 more
openaire   +2 more sources

The Expression of “Single Chain” Antibodies in Transgenic Plants

1995
Abstract Since the first demonstration that plant cells are able to synthesize and secrete antibodies, plants have been considered as alternative hosts for immunoglobulin expression in heterologous systems. Hence plants join the established list of nonlymphoid hosts such as bacteria, yeasts, insect cultured cells, and mammalian cells ...
TAVLADORAKI, Paraskevi, FRANCONI R, BRADBURY A, CATTANEO A, BENVENUTO E.   +4 more
openaire   +2 more sources

Single-chain antibodies as diagnostic tools and therapeutic agents

Thrombosis and Haemostasis, 2009
SummaryOver three decades after the generation of the first mouse monoclonal antibodies by Kohler and Milstein, recombinant antibodies are the fastest growing class of therapeutic proteins. Furthermore, antibodies are key detection reagents in research and diagnostics.
Zur Mühlen, Constantin von, Elverfeldt, Dominik v., Peter, Karlheinz, Hagemeyer, Christoph Eugen   +3 more
openaire   +3 more sources

Single-chain Fv antibody with specificity for Listeria monocytogenes

Journal of Immunological Methods, 2004
Single chain antibodies (scFv) exhibiting specific binding to Listeria monocytogenes strains were isolated from a pool of random scFvs expressed on the surface of filamentous bacteriophages. Positive selection (panning) using L. monocytogenes was used to enrich for phage clones with the desired binding affinity, and negative selection using L.
George C, Paoli, Chin-Yi, Chen, Jeffrey D, Brewster   +2 more
openaire   +2 more sources

Improved expression of single-chain antibodies in Ustilago maydis

Journal of Biotechnology, 2014
To produce the full repertoire of biopharmaceutical proteins, alternative expression platforms are required. Systems that enable secretion of the target protein are favored because this facilitates downstream processing. Ustilago maydis is a promising fungal model organism for future applications in protein expression.
Sarkari, P., Reindl, M., Stock, J., Muller, O., Kahmann, R., Feldbrugge, M., Schipper, K.   +6 more
openaire   +3 more sources