Results 221 to 230 of about 502,506 (259)

Development trends for generation of single-chain antibody fragments

Immunopharmacology and Immunotoxicology, 2014
Recombinant antibodies are increasingly being employed as therapeutic agents especially in combination with anti-cancer drugs. The single-chain antibody fragments are small antigen-binding proteins which provide the most commonly used antibody formats for diagnostic and therapeutic purposes.
Safar, Farajnia, Vahideh, Ahmadzadeh, Asghar, Tanomand, Kamal, Veisi, Shiva Ahdi, Khosroshahi, Leila, Rahbarnia   +5 more
openaire   +2 more sources

Alternative Triggering Molecules and Single Chain Bispecific Antibodies

Journal of Hematotherapy, 1995
Although T cell receptors and Fc receptors are the best known cytotoxic triggering molecules, a number of adhesion molecules recently have been identified as alternative triggering molecules. We discuss how CD44, an adhesion molecule found on all leukocytes and many other cell types, becomes a triggering molecule on NK cells following stimulation with ...
D M, Segal, G, Sconocchia, J A, Titus, C R, Jost, I, Kurucz   +4 more
openaire   +2 more sources

Single chain antibodies that recognize the N-glycosylation site

Archives of Biochemistry and Biophysics, 2004
We aimed to identify antibodies that can recognize the Asn-Xaa-Ser/Thr(NXS/T) N-glycosylation site that guides oligosaccharyltransferase (OT) activity. We used synthetic Asn-Cys-Ser/Thr(NCS/T) tripeptides conjugated to bovine serum albumin to isolate single chain antibody fragments of a variable region (scFv) from the Griffin 1 phage antibody library ...
Masakazu, Kikuchi, Mizuho, Kataoka, Takehiro, Kojima, Tomohisa, Horibe, Kanae, Fujieda, Taiji, Kimura, Toshiki, Tanaka   +6 more
openaire   +2 more sources

Expression of single-chain antibody-barstar fusion in plants

Biochimie, 2007
We successfully cloned and expressed a single-chain antibody (425scFv), that is directed to human epidermal growth factor receptor HER1 (EGFR) in transgenic tobacco plants as a fusion with bacterial barstar gene (425scFv-barstar). Plant-produced recombinant 425scFv-barstar was recovered using barstar-barnase system.
Ekaterina G, Semenyuk, Oleg A, Stremovskiy, Evelina F, Edelweiss, Olga V, Shirshikova, Taras G, Balandin, Yaroslav I, Buryanov, Sergey M, Deyev   +6 more
openaire   +2 more sources

Purification, Characterization, and Biotinylation of Single-Chain Antibodies

2003
The variable region (Fv) portion of an antibody is comprised of the antibody V(H) and V(L) domains and is the smallest antibody fragment containing a complete antigen-binding site. To stabilize the association of the recombinant V(H) and V(L) domains, they have been linked in single-chain Fv constructs with a short peptide that bridges the approx 3.5 ...
openaire   +2 more sources

A Chemiluminescent Immunoassay for the Identification of Functional Single-Chain Antibodies

Analytical Biochemistry, 1995
Random mutagenesis of a template single-chain Fv (scFv) antibody generates a mutant library with members of varying ligand affinities; however, only a small population of these mutants exhibit strong (Kd approximately 1 nM) or improved affinities. We have therefore designed a chemiluminescent sandwich assay which can identify strong ligand-binding ...
I, Lee, J D, Stewart, W, Zhong, S J, Benkovic   +3 more
openaire   +2 more sources

Combinations of Single Chain Variable Fragments From HIV Broadly Neutralizing Antibodies Demonstrate High Potency and Breadth

Frontiers in Immunology, 2021
Kshitij Wagh, Penelope Linda Moore, Rebecca T Van Dorsten   +2 more
exaly  

Epitope Mapping and Computational Analysis of Anti-HPV16 E6 and E7 Antibodies in Single-Chain Format for Clinical Development as Antitumor Drugs

Cancers, 2020
Paola Di Bonito, Luisa Accardi, Barbara Chirullo   +2 more
exaly  

Single-chain Fv Antibodies for Targeting Neurodegenerative Diseases

CNS and Neurological Disorders - Drug Targets, 2018
Khuen Yen Ng, Rhun Yian Koh, Soi Moi Chye   +2 more
exaly  

Antibody, Single Chain

2008
openaire   +1 more source