Results 251 to 260 of about 837,563 (288)

Single-domain antibodies applied as antiviral immunotherapeutics

Journal of Virological Methods, 2023
Viral infections have been the cause of high mortality rates throughout different periods in history. Over the last two decades, outbreaks caused by zoonotic diseases and transmitted by arboviruses have had a significant impact on human health. The emergence of viral infections in different parts of the world encourages the search for new inputs to ...
Nidiane Dantas Reis, Prado, Nairo, Brilhante-Da-Silva, Rosa Maria Oliveira, Sousa, Michelle Suelen da Silva, Morais, Sibele Andrade, Roberto, Marcos Barros, Luiz, Livia Coelho de, Assis, Anna Carolina M, Marinho, Luiz Felipe Lemes de, Araujo, Rafael de Souza, Pontes, Rodrigo Guerino, Stabeli, Carla Freire Celedonio, Fernandes, Soraya Dos Santos, Pereira   +12 more
openaire   +2 more sources

Engineering pH-Sensitive Single-Domain Antibodies

2022
There is increasing interest in expanding an antibody beyond high affinity and specificity. One such feature is custom regulation of the binding event, such as pH-dependent control. Here, we provide a methodology for generating single-domain antibodies (sdAbs) that bind their antigen in a pH-dependent fashion.
Tosha M, Laughlin, James R, Horn
openaire   +2 more sources

Single-Domain Antibody Theranostics on the Horizon

Journal of Nuclear Medicine, 2022
Single-domain antibody (sdAb) is among the most promising vectors for developing molecular imaging tracers. Several sdAb tracers targeting human epidermal growth factor receptor 2 or programmed death ligand 1 have entered clinical practice. However, radiolabeled single-valent sdAbs generally have high kidney retention, limiting their therapeutic ...
Weijun, Wei, Muhsin H, Younis, Xiaoli, Lan, Jianjun, Liu, Weibo, Cai   +4 more
openaire   +2 more sources

Single-domain antibodies

2009
The antigen-binding entity of an antibody, reduced in size to one single domain, is referred to as a "single-domain antibody". Various strategies have been explored with variable success to arrive at functional sinlge-domain antibodies. The potential of single-domain antibodies, as research tools or in medicine, is reflected by the three companies ...
Muyldermans, Serge, Hassanzadeh Ghassabeh, Gholamreza, Saerens, Dirk   +2 more
openaire   +3 more sources

Single-Domain Antibodies for Intracellular Toxin Neutralization

2022
Ricin is a plant-derived toxin with a history as a biothreat agent. The toxin's enzymatic subunit, ricin toxin A chain (RTA), is a ribosome-inactivating protein that, when delivered into the cytoplasm of mammalian cells, arrests protein synthesis with extraordinary efficiency.
Timothy F, Czajka, Nicholas J, Mantis
openaire   +2 more sources

Multivalent Display of Single-Domain Antibodies

2012
Antigen-binding fragments, such as single-domain antibodies (sdAbs), can now be readily isolated by in vitro technologies. Antibody fragment libraries derived from immune or nonimmune sources are presented in a molecular display format, typically phage display, and binders to individual antigens are selected from the libraries by a so-called panning ...
Zhang, J., MacKenzie, C.R.
openaire   +3 more sources

Humanization of Camelid Single-Domain Antibodies

2022
Humanization of therapeutic antibodies derived from animal immunizations is often required to minimize immunogenicity risks in humans, which can cause potentially harmful and serious side effects and reduce antibody efficacy. Humanization is typically applied to conventional monoclonal antibodies derived in rodents as well as single-domain antibodies ...
openaire   +2 more sources

Bispecific Single Domain Antibodies

2011
Monoclonal antibodies are now widely recognized as therapeutic molecules and more than 25 molecules have been approved in the United States and other countries. Despite these successes, the clinical activity of these molecules is still far from optimal and new solutions have to be found, especially in the field of cancer therapy.
Patrick Chames, Daniel Baty
openaire   +1 more source

Production of Single-Domain Antibodies in Pichia pastoris

2022
Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic reagents, and research tools. Functional sdAbs are commonly produced in Escherichia coli, which is a simple and widely used host for production of recombinant proteins. However, there are drawbacks of the
Yusei, Matsuzaki, Kaho, Kajiwara, Wataru, Aoki, Mitsuyoshi, Ueda   +3 more
openaire   +2 more sources

Generation of Single-Domain Antibody-Based Recombinant Immunotoxins

2022
The discovery of single-domain antibodies has opened new avenues for drug development. Single-domain antibodies, also known as nanobodies, can access buried epitopes that are inaccessible to conventional antibodies. These antigen-binding domains have a high level of solubility and stability, which makes them well suited for therapeutic development ...
Bryan D. Fleming, Mitchell Ho
openaire   +2 more sources