Results 121 to 130 of about 665,601 (301)

Liver-Targeting of Interferon-Alpha with Tissue-Specific Domain Antibodies

, 2013
PMCID: PMC3581439This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are ...
Ovecka, M, Coulstock, E, Sepp Armin, Foster, J, Sepp, A, Edward Coulstock (381775), Jerome Burnet (381784), Burnet Jerome, Goodall Laura, Mudd, C, Edward Coulstock, Jane Sosabowski (381776), Mudd Clare, Davies, M, Clare Mudd (381780), Marie Davies, Sosabowski, J, Armin Sepp, Sosabowski Jane, Ovečka Milan, Dunlevy, G, Laura Goodall, Jerome Burnet, Coulstock Edward, Milan Ovečka, Dunlevy Gráinne, Gráinne Dunlevy, Julie Foster, Prince Rob, Foster Julie, Rob Prince, Clare Mudd, Rob Prince (381778), Laura Goodall (381779), Julie Foster (381783), Walker Adam, Goodall, L, Adam Walker, Gráinne Dunlevy (381785), Marie Davies (381782), Adam Walker (381786), Davies Marie, Armin Sepp (381781), Burnet, J, Prince, R, Walker, A, Jane Sosabowski, Milan Ovečka (381777)   +47 more
core   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Novel human single-domain antibodies exert potent anti-tumor activity by targeting EGF-like repeat epitope of EpCAM

Frontiers in Pharmacology
IntroductionEpCAM (Epithelial cell adhesion molecule) is a key cancer stem cell marker involved in cancer progression, making it an important target for both diagnosis and therapy. Despite efforts using anti-EpCAM monoclonal antibodies (mAbs), their anti-
Xiaofeng Zhou, Zhifang Liu, Weixiong Zhang, Lin Dai, Tao Chen, Zexiong Lin, Hong Pan, Qi Qi, Henry Wei   +8 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Cloning and Expression of a Single-Chain Antibody Fragment Specific for Foot-and-Mouth Disease Virus

, 1996
The gene for a single-chain antibody (VHK) to a conformational epitope on the type A12foot-and-mouth disease virus (FMDV) particle was assembled and expressed inEscherichia coli.The VHK, purified from periplasmic extracts immunoprecipitated virus as ...
Mason, P., Kang, A.S., Rieder, E., Berinstein, A., Parsells, R., Kang, A., Baxt, B., Reider, E., Mason, P.W.   +8 more
core   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Single domain antibodies. [PDF]

Lancet (London, England), 1990
openaire   +4 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Single scaffold antibody libraries created with high rates of mutagenesis or diversity focused for peptide recognition

, 2007
textThis dissertation describes several strategies used to create diversity in non-immune antibody libraries. Two of the strategies were used to create two separate peptide focused libraries.
Cobaugh, Christian Wessel, 1971-
core