Results 61 to 70 of about 665,601 (301)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Single-Domain Antibodies and Their Formatting to Combat Viral Infections [PDF]

Antibodies, 2018
Since their discovery in the 1990s, single-domain antibodies (VHHs), also known as Nanobodies®, have changed the landscape of affinity reagents. The outstanding solubility, stability, and specificity of VHHs, as well as their small size, ease of production and formatting flexibility favor VHHs over conventional antibody formats for many applications ...
Dorien De Vlieger, Marlies Ballegeer, Iebe Rossey, Bert Schepens, Xavier Saelens   +4 more
openaire   +4 more sources

Arginine mutation alters binding of a human monoclonal antibody to antigens linked to systemic lupus erythematosus and the antiphospholipid syndrome

, 2007
Objective: Previous studies have shown the importance of somatic mutations and arginine residues in the complementarity-determining regions (CDRs) of pathogenic anti-double-stranded DNA (anti-dsDNA) antibodies in human and murine lupus, and in studies of
Yiannis Ioannou, Lambrianides, A., Anisur Rahman, Isenberg, D.A., Lesley Mason, Giles, I., Ioannou, Y., Latchman, D.S., Mason, L., Jessica J. Manson, Ian Giles, Manson, J.J., David A. Isenberg, David S. Latchman, Rahman, A., Anastasia Lambrianides   +15 more
core   +1 more source

Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester

BMC Biotechnology, 2009
Background Classical bioconjugation strategies for generating antibody-functionalized nanoparticles are non-specific and typically result in heterogeneous compounds that can be compromised in activity.
Raats Jos MH, van Baal Ingrid, Reulen Sanne WA, Merkx Maarten   +3 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Properties, production and applications of camelid single-domain antibody fragments [PDF]

, 2007
Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs or Nanobodies®) have several advantages for biotechnological ...
de Haard, H.J., Haard, H.J., de, Harmsen, M.M., M. M. Harmsen, H. J. De Haard   +4 more
core   +1 more source

Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein. [PDF]

PLoS ONE, 2016
Single-domain antibodies derived from the unique New Antigen Receptor found in sharks have numerous potential applications, ranging from diagnostic reagents to therapeutics.
George P Anderson, Daniel D Teichler, Dan Zabetakis, Lisa C Shriver-Lake, Jinny L Liu, Stephen G Lonsdale, Sarah A Goodchild, Ellen R Goldman   +7 more
doaj   +1 more source

Bamboo Shark as a Small Animal Model for Single Domain Antibody Production

Frontiers in Bioengineering and Biotechnology, 2021
The development of shark single domain antibodies (sdAbs) is hindered by the high cost and tediousness of large-sized shark farming. Here, we demonstrated white-spotted bamboo sharks (Chiloscyllium plagiosum) being cultivated commercially as a promising ...
Likun Wei, Meiniang Wang, Meiniang Wang, Haitao Xiang, Haitao Xiang, Haitao Xiang, Yuan Jiang, Jinhua Gong, Dan Su, M. A. R. Al Azad, Hongming Dong, Hongming Dong, Hongming Dong, Limin Feng, Jiajun Wu, Leo Lai Chan, Leo Lai Chan, Naibo Yang, Naibo Yang, Naibo Yang, Jiahai Shi, Jiahai Shi, Jiahai Shi, Jiahai Shi, Jiahai Shi   +24 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Computational maturation of a single-domain antibody against Aβ42 aggregation.

, 2022
The expansion of structural databases and the increase in computing power are enabling approaches for antibody discovery based on computational design. It has already been shown that it is possible to use this approach to generate antibodies for specific

core   +1 more source