Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Optimizing single domain antibody production using signal peptides
Signal peptides are short sequences at the N-terminus of secretory proteins that encode information about secretory pathway and target location of a protein. Synthesis of recombinant secretory proteins depends on signal peptides for proper processing and
Hoque, Ragheeb
core +4 more sources
Computational maturation of a single-domain antibody against Aβ42 aggregation. [PDF]
, 2022 The expansion of structural databases and the increase in computing power are enabling approaches for antibody discovery based on computational design. It has already been shown that it is possible to use this approach to generate antibodies for specific
core +2 more sources
Bamboo Shark as a Small Animal Model for Single Domain Antibody Production
Frontiers in Bioengineering and Biotechnology, 2021 The development of shark single domain antibodies (sdAbs) is hindered by the high cost and tediousness of large-sized shark farming. Here, we demonstrated white-spotted bamboo sharks (Chiloscyllium plagiosum) being cultivated commercially as a promising ...
Likun Wei +24 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system [PDF]
, 2002 The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor ...
Daly, Stephen J. +6 more
core +2 more sources
Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein. [PDF]
PLoS ONE, 2016 Single-domain antibodies derived from the unique New Antigen Receptor found in sharks have numerous potential applications, ranging from diagnostic reagents to therapeutics.
George P Anderson +7 more
doaj +1 more source
Prospecting the protein design landscape
FEBS Letters, EarlyView.This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona +4 more
wiley +1 more source
Antibody Conjugates via Disulfide Bridging: Towards therapeutic and diagnostic applications [PDF]
, 2015 Antibodies play a prominent role in chemical and biological research and the largest application of chemical bioconjugation reagents is in the production of antibody conjugates.
Hull, EA
core
Adaptive frequency-domain equalization for single-carrier MIMO systems [PDF]
, 2004 Channel estimation and tracking pose real problems in wideband single-carrier wireless communication systems employing multiple transmit and receive antennas. An alternative to estimating the channel is to adaptively equalize the received symbols.
Coon, J +7 more
core +1 more source