Results 11 to 20 of about 258,562 (263)

A single-nucleus RNA-sequencing pipeline to decipher the molecular anatomy and pathophysiology of human kidneys [PDF]

Nature Communications, 2019
Single-cell studies in solid tissues remain challenging and have benefited from the development of single-nuclei RNA sequencing strategies. Here Lake et al.
Blue B. Lake, Song Chen, Masato Hoshi, Nongluk Plongthongkum, Diane Salamon, Amanda Knoten, Anitha Vijayan, Ramakrishna Venkatesh, Eric H. Kim, Derek Gao, Joseph Gaut, Kun Zhang, Sanjay Jain   +12 more
doaj   +4 more sources

SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology [PDF]

Genome Biology, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku   +8 more
doaj   +3 more sources

Benchmarking of computational demultiplexing methods for single-nucleus RNA sequencing data. [PDF]

Brief Bioinform
Abstract Single-nucleus RNA sequencing (snRNA-Seq) has transformed our understanding of complex tissues, providing insights into cellular composition and heterogeneity in gene expression between cells, and their alterations in development and disease. High costs however constrain the number of samples analysed.
Fu Y, Youness M, Virzì A, Song X, Tubeeckx MRL, De Keulenaer GW, Heidbuchel H, Segers VFM, Sipido KR, Thienpont B, Roderick HL.   +10 more
europepmc   +4 more sources

Sex-specific proximal tubular cell differentiation pathways identified by single-nucleus RNA sequencing

Scientific Reports, 2023
Postnatal kidney growth is substantial and involves expansion in kidney tubules without growth of new nephrons, which are the functional units of the kidney. Proliferation and differentiation pathways underpinning nephron elongation are not well defined.
Yueh-An Lu, Tanya Smith, Sumukh Deshpande, Chia-Te Liao, Bnar Talabani, Irina Grigorieva, Anna Mason, Robert Andrews, Timothy Bowen, Philip R. Taylor, Donald Fraser   +10 more
doaj   +3 more sources

A simplified preparation method for single-nucleus RNA-sequencing using long-term frozen brain tumor tissues [PDF]

Scientific Reports
Single-cell RNA-sequencing has provided intriguing new insights into research areas such as developmental processes and tumor heterogeneity. Most approaches, however, rely on the availability of fresh surgical specimens, thereby dramatically reducing the
Kati J. Ernst, Konstantin Okonechnikov, Josephine Bageritz, Ashwyn A. Perera, Jan-Philipp Mallm, Andrea Wittmann, Kendra K. Maaß, Svenja Leible, Michael Boutros, Stefan M. Pfister, Marc Zuckermann, David T. W. Jones   +11 more
doaj   +2 more sources

Single-nucleus RNA-sequencing of autosomal dominant Alzheimer disease and risk variant carriers

Nature Communications, 2023
Genetic studies of Alzheimer disease (AD) have prioritized variants in genes related to the amyloid cascade, lipid metabolism, and neuroimmune modulation. However, the cell-specific effect of variants in these genes is not fully understood.
Logan Brase, Shih-Feng You, Ricardo D’Oliveira Albanus, Jorge L. Del-Aguila, Yaoyi Dai, Brenna C. Novotny, Carolina Soriano-Tarraga, Taitea Dykstra, Maria Victoria Fernandez, John P. Budde, Kristy Bergmann, John C. Morris, Randall J. Bateman, Richard J. Perrin, Eric McDade, Chengjie Xiong, Alison M. Goate, Martin Farlow, Dominantly Inherited Alzheimer Network (DIAN), Greg T. Sutherland, Jonathan Kipnis, Celeste M. Karch, Bruno A. Benitez, Oscar Harari   +23 more
doaj   +4 more sources

Single‐Nucleus RNA‐Sequencing in Brain Tissue

Current Protocols, 2023
AbstractNeuroscience research greatly benefits from single‐cell sequencing technologies, which can reveal transcriptional alterations on a cellular level. However, preparing single‐cell suspensions is technically challenging, requires experience, and has several limitations that can influence the transcriptional readout. Performing sequencing of single
Rebecca Waag, Johannes Bohacek
openaire   +4 more sources

Single Nucleus RNA Sequencing of Presumed Human Heart Failure Myocardium

Journal of Molecular and Cellular Cardiology Plus
Deborah Eaton, Kenneth Bedi, Ryan Pfeiffer, Nathan Tucker, Kenneth Margulies   +4 more
doaj   +2 more sources

Gene length is a pivotal feature to explain disparities in transcript capture between single transcriptome techniques

Frontiers in Bioinformatics, 2023
The scale and capability of single-cell and single-nucleus RNA-sequencing technologies are rapidly growing, enabling key discoveries and large-scale cell mapping operations.
Ricardo R. Pavan, Fabiola Diniz, Samir El-Dahr, Giovane G. Tortelote   +3 more
doaj   +1 more source

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing [PDF]

Journal of Visualized Experiments, 2021
The kidneys regulate diverse biological processes such as water, electrolyte, and acid-base homeostasis. Physiological functions of the kidney are executed by multiple cell types arranged in a complex architecture across the corticomedullary axis of the organ.
Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K.M.   +6 more
openaire   +4 more sources