Results 31 to 40 of about 258,562 (263)

Single‐nucleus RNA sequencing of plant tissues using a nanowell‐based system [PDF]

The Plant Journal, 2021
SUMMARYSingle‐cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nucleus isolation combined with nanowell‐based library preparation. Our method enables the transcriptome analysis of thousands of individual plant nuclei.
Sunaga‐Franze, Daniele Y., Muino, Jose M., Braeuning, Caroline, Xu, Xiaocai, Zong, Minglei, Smaczniak, Cezary, Yan, Wenhao, Fischer, Cornelius, Vidal, Ramon, Kliem, Magdalena, Kaufmann, Kerstin, Sauer, Sascha   +11 more
openaire   +3 more sources

Nanopipettes as Monitoring Probes for the Single Living Cell: State of the Art and Future Directions in Molecular Biology. [PDF]

, 2018
Examining the behavior of a single cell within its natural environment is valuable for understanding both the biological processes that control the function of cells and how injury or disease lead to pathological change of their function.
Bulbul, Gonca, Chaves, Gepoliano, Olivier, Joseph, Ozel, Rifat Emrah, Pourmand, Nader   +4 more
core   +2 more sources

A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons [PDF]

, 2018
Mutations in fused in sarcoma (FUS) cause amyotrophic lateral sclerosis (ALS). FUS is a multifunctional protein involved in the biogenesis and activity of several types of RNAs, and its role in the pathogenesis of ALS may involve both direct effects of
Biscarini, Silvia, Bozzoni, Irene, Caffarelli, Elisa, Capauto, Davide, Colantoni, Alessio, Laneve, Pietro, Lu, Lei, Morlando, Mariangela, Peruzzi, Giovanna, Santini, Tiziana, Shneider, Neil A.   +10 more
core   +2 more sources

Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

Genome Biology, 2020
Background Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single
Elena Denisenko, Belinda B. Guo, Matthew Jones, Rui Hou, Leanne de Kock, Timo Lassmann, Daniel Poppe, Olivier Clément, Rebecca K. Simmons, Ryan Lister, Alistair R. R. Forrest   +10 more
doaj   +1 more source

On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei [PDF]

, 2014
Background: Although technical advances in genomics and proteomics research have yielded a better understanding of the coding capacity of a genome, one major challenge remaining is the identification of all expressed proteins, especially those less than ...
Butter, F., Ericson, M., Janes, M., Mann, M., Tschudi, C., Ullu, E.   +5 more
core   +2 more sources

Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics

STAR Protocols, 2021
Summary: Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction.
Elvira Laila Van Hauwaert, Ellen Gammelmark, Anitta Kinga Sárvári, Lena Larsen, Ronni Nielsen, Jesper Grud Skat Madsen, Susanne Mandrup   +6 more
doaj   +1 more source

CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. [PDF]

, 2018
Hexanucleotide-repeat expansions in the C9ORF72 gene are the most common cause of amyotrophic lateral sclerosis and frontotemporal dementia (c9ALS/FTD).
A Berson, A Jovičić, AA Dominguez, Aaron D. Gitler, AC Elden, AD Gitler, AD Gitler, AD Gitler, AE Renton, AM Jablonski, Amy Li, Ana Jovičić, AS Ng, BD Freibaum, C Sidrauski, C Sidrauski, C. Kimberly Tsui, D Ron, David W. Morgens, DW Morgens, DW Morgens, E Yeger-Lotem, EM Lynes, EY Son, FB Gao, Gregor Bieri, H Koike-Yusa, HJ Kim, HJ Kim, I Kwon, IR Mackenzie, IR Mackenzie, J Couthouis, J Joung, James Ousey, JC Christianson, JG Wideman, JP Taylor, Julien Couthouis, Justin K. Ichida, K Kanekura, K Mori, K Zhang, KH Lee, KM Green, KY Shi, L Ellgaard, L Huang, LA Becker, LA Gilbert, LA Gilbert, M Armakola, M DeJesus-Hernandez, M Neumann, M Prudencio, MA Horlbeck, Marc Tessier-Lavigne, MC Jonikas, MH Schludi, MI Love, Michael C. Bassik, Michael S. Haney, NF Liachko, Nicholas J. Kramer, Nicholas T. Hertz, O Shalem, OM Peters, PE Ash, Q Zhou, R Dafinca, R Lopez-Gonzalez, R Seijffers, RJ Platt, RM Deans, Rosanna Ma, S Boeynaems, S Boeynaems, S Ju, S Konermann, S Mizielinska, S Treusch, SC Ling, SJ Zhang, T Wang, T Westergard, T Zu, TF Gendron, W Cheng, X Wen, Y Lin, Y Zhou, Yingxiao Shi, YJ Zhang, YS Davidson, Z Shevtsova, Z Sun   +95 more
core   +1 more source

High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq

Nature Communications, 2023
Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank for clinical history and follow-up data. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues.
Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, Shengyu Ni, Fangru Lu, Zhaolun Wang, Hao Yang, Ling Dong, Feng Chen, Hong Zhang, Yu Chen, Jiong Liu, Dandan Zhang, Longjiang Fan, Guoji Guo, Yongcheng Wang   +22 more
doaj   +1 more source

Systematic Methods for Isolating High Purity Nuclei from Ten Important Plants for Omics Interrogation

Cells, 2022
Recent advances in developmental biology have been made possible by using multi-omic studies at single cell resolution. However, progress in plants has been slowed, owing to the tremendous difficulty in protoplast isolation from most plant tissues and/or
Ming-Chao Yang, Zi-Chen Wu, Liang-Liang Huang, Farhat Abbas, Hui-Cong Wang   +4 more
doaj   +1 more source

Quantification and statistical modeling of droplet-based single-nucleus RNA-sequencing data

Biostatistics, 2023
Summary In complex tissues containing cells that are difficult to dissociate, single-nucleus RNA-sequencing (snRNA-seq) has become the preferred experimental technology over single-cell RNA-sequencing (scRNA-seq) to measure gene expression.
Albert Kuo, Kasper D Hansen, Stephanie C Hicks   +2 more
openaire   +2 more sources