Syncrip/hnRNP Q is required for activity-induced Msp300/Nesprin-1 expression and new synapse formation. [PDF]
, 2019 Memory and learning involve activity-driven expression of proteins and cytoskeletal reorganization at new synapses, requiring posttranscriptional regulation of localized mRNA a long distance from corresponding nuclei.
Davis, Ilan +6 more
core +4 more sources
Virtual in situs: Sequencing mRNA from cryo-sliced Drosophila embryos to determine genome-wide spatial patterns of gene expression [PDF]
, 2013 Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development.
Combs, Peter A., Eisen, Michael B.
core +8 more sources
FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants
Genome Biology, 2021 The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq,
Yanping Long +9 more
doaj +1 more source
Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle [PDF]
, 2015 The voltage-gated calcium channel (Cav) β1a subunit (Cavβ1a) plays an important role in excitation-contraction coupling (ECC), a process in the myoplasm that leads to muscle-force generation. Recently, we discovered that the Cavβ1a subunit travels to the
Delbono, Osvaldo +7 more
core +2 more sources
CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]
, 2018 The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad +7 more
core +1 more source
Molecular bases of circadian magnesium rhythms across eukaryotes
FEBS Letters, EarlyView.Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley +1 more source
Single-nuclei transcriptomics enable detection of somatic variants in patient brain tissue
Scientific Reports, 2023 Somatic variants are a major cause of human disease, including neurological disorders like focal epilepsies, but can be challenging to study due to their mosaicism in bulk tissue biopsies.
Sydney E. Townsend +6 more
doaj +1 more source
Frontiers in Molecular Neuroscience, 2023 IntroductionOligodendrocyte progenitor cells (OPCs) are vital for neuronal myelination and remyelination in the central nervous system. While the molecular mechanisms involved in OPCs’ differentiation and maturation are not completely understood, GABA is
Berenice A. Gutierrez +4 more
doaj +1 more source
, 2019 The cellular and the molecular mechanisms by which long noncoding RNAs (lncRNAs) may regulate presynaptic function and neuronal activity are largely unexplored.
Bansal, V. +13 more
core +1 more source
The lncRNA landscape of breast cancer reveals a role for DSCAM-AS1 in breast cancer progression. [PDF]
, 2016 Molecular classification of cancers into subtypes has resulted in an advance in our understanding of tumour biology and treatment response across multiple tumour types. However, to date, cancer profiling has largely focused on protein-coding genes, which
Backofen, Rolf +27 more
core +1 more source