Results 221 to 230 of about 367,400 (264)

Quantitative Amplification of Single-Stranded DNA

2012
Single-stranded DNA (ssDNA) intermediates play an important role in processes such as DNA replication and homologous recombination, DNA damage responses, and DNA repair. Using quantitative amplification of ssDNA (QAOS), ssDNA arising during various cellular processes in complex genomes can be quantified at numerous single-copy and repetitive loci. QAOS
Eva-Maria, Holstein, David, Lydall
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Hybridization with Nanostructures of Single-Stranded DNA

Langmuir, 2005
Nanostructures of single-stranded DNA (ssDNA) were produced within alkanethiol self-assembled monolayers using nanografting, an atomic force microscopy (AFM) based lithography technique. Next, variations of the fabrication parameters, such as the concentration of ssDNA or lines per frame, allowed for the regulation of the density of ssDNA molecules ...
Maozi, Liu, Gang-Yu, Liu
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Crosslinking of Single-Stranded DNA to Resins

2003
Specific fragments of single-stranded DNA may be covalently immobilized to solid supports for various reasons. The support can be used as an affinity column for purifying DNA from a test sample, to detect viral or bacterial DNAs, or for antenatal diagnosis of genetic diseases such as sickle cell anemia using a sandwich hybridization method developed in
J L, Woodhead, J A, Langdale, A D, Malcolm   +2 more
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Single Stranded DNA in Lymphocyte Chromosomes

Nature, 1967
The distribution of single stranded DNA in lymphocytes has been examined by an immuno-fluorescence technique. Chromosomal genes seem to be active in these cells throughout the cell cycle.
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Single-Stranded DNA Family Shuffling

2004
Publisher Summary This chapter examines the various aspects of single-stranded DNA family shuffling. DNA shuffling is a technique that allows accelerated and directed protein evolution in vitro . This technique has been shown to be powerful in combining independently isolated mutations of a gene into a single progeny. By using the sense strand ssDNA
Osamu, Kagami, Miho, Kikuchi, Shigeaki, Harayama   +2 more
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Nanopore Unstacking of Single‐Stranded DNA Helices

Small, 2007
Time to unwind: The force-induced unstacking of individual single-stranded homopolymeric DNA helices is demonstrated during their translocation through an -hemolysin protein nanopore by electrophoresis (see picture). The unstacking energy and sequence-specific cooperativity are inferred from an analysis of the translocation kinetics.
Chen, Peng, Li, Chang Ming
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Electron microscopy of single-stranded DNA

Journal of Molecular Biology, 1970
A method is described for the quantitative study of single-stranded DNA by electron microscopy. It permits the determination of the size of single-stranded DNA with sample standard deviations between 5 and 8%. Since 80 to 100% of the molecules in a given field are usually measurable, size distributions can also be obtained. The linear density of single-
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Conformational changes of single-stranded DNA

Journal of Molecular Biology, 1969
Abstract The conformation of intact, essentially monodisperse, single-stranded T7 DNA was studied in solutions between 0.001 and 1 m -NaCl at neutral and alkaline pH and temperatures between 5 and 35 °C. Over-all shape is assessed by measurement of S 20,w 0 , and base stacking by absorbance at 260 mμ.
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Single-Stranded DNA Phage Origins

1988
Since the rediscovery of bacteriophage o X174 in the 1950 s by Robert Sinsheimer (1959), the single-stranded DNA phages have been widely used as model systems in molecular biology. o X174 can be considered as their “ godfather. ” The single-stranded DNA bacteriophages are divided into two classes based on the morphology of their representatives, either
P D, Baas, H S, Jansz
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DNA single-strand breaks and neurodegeneration

DNA Repair, 2004
The association of human genetic disorders with defects in the DNA damage response is well established. Most of the major DNA repair pathways are represented by diseases in which that pathway is absent or impaired, including those responsible for repairing DNA double-strand breaks.
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