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Single-stranded DNA damage: Protecting the single-stranded DNA from chemical attack
DNA Repair, 2020Cellular processes, such as DNA replication, recombination and transcription, require DNA strands separation and single-stranded DNA is formation. The single-stranded DNA is promptly wrapped by human single-stranded DNA binding proteins, replication protein A (RPA) complex.
Roy Anindya
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Replication Fork Activation Is Enabled by a Single-Stranded DNA Gate in CMG Helicase
Cell, 2019 SUMMARY The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds) DNA at origins, yet must transition to single-stranded (ss) DNA for helicase action.
Michael R Wasserman +2 more
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Thermophoresis of single stranded DNA
ELECTROPHORESIS, 2010AbstractThe manipulation and analysis of biomolecules in native bulk solution is highly desired; however, few methods are available. In thermophoresis, the thermal analog to electrophoresis, molecules are moved along a microscopic temperature gradient. Its theoretical foundation is still under debate, but practical applications for analytics in biology
Philipp, Reineck +2 more
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Isolation of Single‐Stranded DNA
Current Protocols in Molecular Biology, 2014AbstractSingle‐stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies. Asymmetric PCR or exonuclease digestion followed by urea gel separation and isolation on streptavidin‐coated magnetic beads are commonly used for this purpose. These two methods may not yield large amounts of highly purified ssDNA.
Yuji, Wakimoto +2 more
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The Single-Stranded DNA Phages
CRC Critical Reviews in Microbiology, 1975(1975). The Single-Stranded DNA Phages. CRC Critical Reviews in Microbiology: Vol. 4, No. 2, pp. 161-223.
David Tilton Denhardt, Peter Model
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Breaking and joining single-stranded DNA: the HUH endonuclease superfamily
Nature Reviews Microbiology, 2013 Michael Chandler +2 more
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Tight Single-Stranded DNA Knots
Journal of Biomolecular Structure and Dynamics, 1993Trefoil (3(1)) and figure-8 (4(1)) knots have been synthesized from DNA molecules containing two single-turn helical domains, linked by four oligodeoxythymidine linkers. Both topologies are derived from the same DNA molecule. The tightest knots are fashioned by minimizing the lengths of the linkers.
H, Wang, S M, Du, N C, Seeman
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Chain persistency in single-stranded DNA
Physical Review E, 2004We develop a theoretical approach to hairpin-loop formation of single-stranded (ss) DNA by treating the strand as a two-state system in which bases are either "stacked" or "unstacked." The looping kinetics of ssDNA is shown to be intrinsically different from that of a wormlike chain; it is mainly controlled by stacking-breakage probability, not by the ...
Anirban, Sain +3 more
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Scientific American, 1962
The molecule of the hereditary material deoxyribonucleic acid (DNA) normally consists of two interwoven strands. The discovery of a form with only one strand promises to clarify how the molecule replicates.
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The molecule of the hereditary material deoxyribonucleic acid (DNA) normally consists of two interwoven strands. The discovery of a form with only one strand promises to clarify how the molecule replicates.
openaire +3 more sources