Results 71 to 80 of about 852,815 (296)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection. [PDF]

, 2019
The interplay between a highly polymorphic set of MHC-I alleles and molecular chaperones shapes the repertoire of peptide antigens displayed on the cell surface for T cell surveillance.
Choi, Hannah, Devlin, Christine A, Flores-Solis, David, McShan, Andrew C, Moschidi, Danai, Overall, Sarah A, Park, Jihye, Procko, Erik, Sgourakis, Nikolaos G, Toor, Jugmohit S, Tripathi, Sarvind   +10 more
core  

Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2 [PDF]

, 2015
Ets-2, like its closely related homologue Ets-1, is a member of the Ets family of DNA binding transcription factors. Both proteins are subject to multiple levels of regulation of their DNA binding and transactivation properties.
Adams, Babayeva, Babayeva, Baillat, Baillat, Christopher D.O. Cooper, Cowley, Degnan, Desjardins, Emsley, Garvie, Goetz, Guen, Hagman, Hazel Aitkenhead, Hollenhorst, Jonsen, Joseph A Newman, Kabsch, Lamber, Lee, Lee, Lim, Opher Gileadi, Petersen, Pufall, Savitsky, Shaikhibrahim, Vagin, Venanzoni, Wang, Wang, Wasylyk, Wei   +33 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Intraocular Penetration of a vNAR: In Vivo and In Vitro VEGF165 Neutralization

Marine Drugs, 2018
Variable new antigen receptor domain (vNAR) antibodies are novel, naturally occurring antibodies that can be isolated from naïve, immune or synthetic shark libraries. These molecules are very interesting to the biotechnology and pharmaceutical industries
Tanya A. Camacho-Villegas, María Teresa Mata-González, Walter García-Ubbelohd, Linda Núñez-García, Carolina Elosua, Jorge F. Paniagua-Solis, Alexei F. Licea-Navarro   +6 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Therapeutic Potential of Shark Anti-ICOSL VNAR Domains is Exemplified in a Murine Model of Autoimmune Non-Infectious Uveitis

Frontiers in Immunology, 2017
Induced costimulatory ligand (ICOSL) plays an important role in the activation of T cells through its interaction with the inducible costimulator, ICOS. Suppression of full T cell activation can be achieved by blocking this interaction and has been shown
Marina Kovaleva, Katherine Johnson, John Steven, Caroline J. Barelle, Andrew Porter, Andrew Porter   +5 more
doaj   +1 more source

Computational and Rational Design of Single-Chain Antibody against Tick-Borne Encephalitis Virus for Modifying Its Specificity

Viruses, 2021
Tick-borne encephalitis virus (TBEV) causes 5−7 thousand cases of human meningitis and encephalitis annually. The neutralizing and protective antibody ch14D5 is a potential therapeutic agent. This antibody exhibits a high affinity for binding with the D3
Ivan K. Baykov, Pavel Y. Desyukevich, Ekaterina E. Mikhaylova, Olga M. Kurchenko, Nina V. Tikunova   +4 more
doaj   +1 more source

Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method. [PDF]

, 2016
Adenosine deamination is one of the most prevalent post-transcriptional modifications in mRNA. In humans, ADAR1 and ADAR2 catalyze this modification and their malfunction correlates with disease. Recently our laboratory reported crystal structures of the
Beal, Peter A, Wang, Yuru
core   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source