Results 61 to 70 of about 478,505 (298)

A novel tri-functional antibody fusion protein with improved pharmacokinetic properties generated by fusing a bispecific single-chain diabody with an albumin-binding domain from streptococcal protein G [PDF]

Protein Engineering Design and Selection, 2007
The therapeutic application of small recombinant antibody molecules is often limited by a short serum half-life. In order to improve the pharmacokinetic properties, we have investigated a strategy utilizing fusion with an albumin-binding domain (ABD) from streptococcal protein G.
Roland, Stork, Dafne, Müller, Roland E, Kontermann   +2 more
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Identification of functional differences between recombinant human ? and ? cardiac myosin motors [PDF]

, 2012
The myosin isoform composition of the heart is dynamic in health and disease and has been shown to affect contractile velocity and force generation.
Rezavandi, Heresh, Rezavandi, H., Leslie A. Leinwand, Geeves, M., Leinwand, Leslie A., Leinwand, L., Geeves, Michael A., Marieke J. Bloemink, Heresh Rezavandi, Deacon, John C., Bloemink, Marieke J., Deacon, J., John C. Deacon, Bloemink, M., Michael A. Geeves   +14 more
core   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Study of protein structure by electron microscopy and single particle image processing [PDF]

, 2015
The molecular assemblies form an important role in the understanding the machinery of living cells. These assemblies with thickness less than 1000nm and exhibiting different shapes, size and biochemical states are studied using Transmission Electron ...
Dandey, Venkata Prasad
core   +1 more source

Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery

mAbs
Heavy chain-only antibodies have found many applications where conventional heavy-light heterodimeric antibodies are not favorable. Heavy chain-only antibodies with their single antigen-binding domain offer the advantage of a smaller size and higher ...
Christine N. Vuong, Kevin M. Reynolds, Gerry S. Rivera, Baisen Zeng, Zahra Karimpourkalou, Manith Norng, Yulei Zhang, Robayet Chowdhury, Darlene Pedersen, Melissa Pantoja, Ellen Collarini, Swetha Garimalla, Shelley Izquierdo, Eric G. Vajda, Brett Antonio, Devendra B. Srivastava, Marie-Cecile van de Lavoir, Yasmina Abdiche, William Harriman, Philip A. Leighton   +19 more
doaj   +1 more source

A Novel Sequence in AP180 and CALM Promotes Efficient Clathrin Binding and Assembly. [PDF]

PLoS ONE, 2016
The clathrin heavy chain N-terminal domain interacts with endocytic adapter proteins via clathrin binding motifs to assemble clathrin triskelia into cages. However, the precise mechanism of clathrin assembly is not yet known.
Lia Moshkanbaryans, Jing Xue, Jesse Ray Wark, Phillip James Robinson, Mark Evan Graham   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Two polymorphisms facilitate differences in plasticity between two chicken major histocompatibility complex class I proteins

, 2014
Major histocompatibility complex class I molecules (MHC I) present peptides to cytotoxic T-cells at the surface of almost all nucleated cells. The function of MHC I molecules is to select high affinity peptides from a large intracellular pool and they ...
van Hateren, Andy, Elliott, Tim, Tim Elliott (201327), Alistair Bailey, Jörn M Werner, Bailey, Alistair, Werner, Jörn M., Tim Elliott, Jörn M. Werner (524999), Andy van Hateren, Alistair Bailey (524997), Andy van Hateren (524998)   +11 more
core   +1 more source

A Single Tyrosine Residue in the Membrane-proximal Domain of the Granulocyte-Macrophage Colony-stimulating Factor, Interleukin (IL)-3, and IL-5 Receptor Common β-Chain Is Necessary and Sufficient for High Affinity Binding and Signaling by All Three Ligands [PDF]

Journal of Biological Chemistry, 1996
The beta-chain of the granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and interleukin-5 (IL-5) receptors functions as a communal receptor subunit and is often referred to as beta common (betac). Analogous to other shared receptor subunits including gp130 and the IL-2R gamma chain, betac mediates high affinity binding ...
J M, Woodcock, C J, Bagley, B, Zacharakis, A F, Lopez   +3 more
openaire   +2 more sources