Individual globular domains and domain unfolding visualized in overstretched titin molecules with atomic force microscopy. [PDF]
Titin is a giant elastomeric protein responsible for the generation of passive muscle force. Mechanical force unfolds titin's globular domains, but the exact structure of the overstretched titin molecule is not known.
Kellermayer Miklós +5 more
core +1 more source
Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. [PDF]
Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C.
Ali Riazi +10 more
doaj +1 more source
Expression of recombinant multi-coloured fluorescent antibodies in
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V. +26 more
core +1 more source
Structure of the Single-lobe Myosin Light Chain C in Complex with the Light Chain-binding Domains of Myosin-1C Provides Insights into Divergent IQ Motif Recognition [PDF]
Myosin light chains are key regulators of class 1 myosins and typically comprise two domains, with calmodulin being the archetypal example. They bind IQ motifs within the myosin neck region and amplify conformational changes in the motor domain. A single lobe light chain, myosin light chain C (MlcC), was recently identified and shown to specifically ...
David N, Langelaan +7 more
openaire +2 more sources
The hyaluronan-binding serine protease from human plasma cleaves HMW and LMW kininogen and releases bradykinin [PDF]
The influence of the hyaluronanbinding protease (PHBSP), a plasma enzyme with FVII- and pro-urokinase-activating potency, on components of the contact phase (kallikrein/kinin) system was investigated.
Fink, E. +4 more
core +1 more source
Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core +1 more source
Llama-Derived Single Domain Antibodies to Build Multivalent, Superpotent and Broadened Neutralizing Anti-Viral Molecules [PDF]
For efficient prevention of viral infections and cross protection, simultaneous targeting of multiple viral epitopes is a powerful strategy. Llama heavy chain antibody fragments (VHH) against the trimeric envelope proteins of Respiratory Syncytial Virus (
Schepens, B +94 more
core +1 more source
Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1 [PDF]
The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex.
Mills, Deryck J. +11 more
core +1 more source
Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo +13 more
core +1 more source
The Escherichia coli serotype O9a O-antigen polysaccharide (O-PS) is a model for glycan biosynthesis and export by the ATP-binding cassette transporter-dependent pathway.
James H. Naismith +11 more
core +1 more source

