Results 31 to 40 of about 852,815 (296)

Structure of a Pheromone Receptor-Associated MHC Molecule with an Open and Empty Groove [PDF]

, 2005
Neurons in the murine vomeronasal organ (VNO) express a family of class Ib major histocompatibility complex (MHC) proteins (M10s) that interact with the V2R class of VNO receptors.
Bjorkman, Pamela J., Dulac, Catherine, Huey-Tubman, Kathryn E., Olson, Rich   +3 more
core   +3 more sources

Observation and Characterization of the Interaction between a Single Immunoglobulin Binding Domain of Protein L and Two Equivalents of Human κ Light Chains [PDF]

Journal of Biological Chemistry, 2004
Detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single Ig-binding domain of protein L from Peptostreptococcus magnus binding to kappa light chain are due to the ...
Housden, N.G., Harrison, S., Housden, H.R., Thomas, K.A., Beckingham, J.A., Roberts, S.E., Bottomley, S.P., Graille, M., Stura, E., Gore, M.G.   +9 more
openaire   +4 more sources

The role of the C-domain of bacteriophage T4 gene 32 protein in ssDNA binding and dsDNA helix-destabilization: Kinetic, single-molecule, and cross-linking studies. [PDF]

PLoS ONE, 2018
The model single-stranded DNA binding protein of bacteriophage T4, gene 32 protein (gp32) has well-established roles in DNA replication, recombination, and repair. gp32 is a single-chain polypeptide consisting of three domains.
Kiran Pant, Brian Anderson, Hendrik Perdana, Matthew A Malinowski, Aye T Win, Christopher Pabst, Mark C Williams, Richard L Karpel   +7 more
doaj   +1 more source

VNARs: An Ancient and Unique Repertoire of Molecules That Deliver Small, Soluble, Stable and High Affinity Binders of Proteins [PDF]

, 2015
Peer reviewedPublisher ...
Clem, Flajnik, Makrides, Marchalonis, Müller   +4 more
core   +3 more sources

Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronectin in Pichia pastoris [PDF]

Protein Expression and Purification, 2001
The Pichia pastoris expression system was used to produce functionalized single-chain antibody fragments (scFv) directed against the ED-B domain of the B-fibronectin (B-Fn) isoform which was found to be present only in newly formed blood vessels during tumor angiogenesis.
Marty, C, Scheidegger, P, Ballmer-Hofer, K, Klemenz, R, Schwendener, R   +4 more
openaire   +2 more sources

Arginine mutation alters binding of a human monoclonal antibody to antigens linked to systemic lupus erythematosus and the antiphospholipid syndrome [PDF]

, 2007
Objective: Previous studies have shown the importance of somatic mutations and arginine residues in the complementarity-determining regions (CDRs) of pathogenic anti-double-stranded DNA (anti-dsDNA) antibodies in human and murine lupus, and in studies of
Giles, I., Ioannou, Y., Isenberg, D.A., Lambrianides, A., Latchman, D.S., Manson, J.J., Mason, L., Rahman, A.   +7 more
core   +1 more source

Recombinant rabbit single‐chain antibodies bind to the catalytic and C‐terminal domains of HIV‐1 integrase protein and strongly inhibit HIV‐1 replication [PDF]

Biotechnology and Applied Biochemistry, 2012
AbstractThe human immunodeficiency virus type 1 (HIV‐1) integrase (IN) protein plays an important role during the early stages of the retroviral life cycle and therefore is an attractive target for therapeutic intervention. We immunized rabbits with HIV‐1 IN protein and developed a combinatorial single‐chain variable fragment (scFv) library against IN.
Frederico Aires, da Silva, Min, Li, Sylvie, Rato, Sara, Maia, Rui, Malhó, Kylie, Warren, David, Harrich, Robert, Craigie, Carlos, Barbas, Joao, Goncalves   +9 more
openaire   +2 more sources

Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein

eLife, 2022
As the only major retrograde transporter along microtubules, cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes.
Kayla A Jara, Nikolaus M Loening, Patrick N Reardon, Zhen Yu, Prajna Woonnimani, Coban Brooks, Cat H Vesely, Elisar J Barbar   +7 more
doaj   +1 more source

Selective engagement of FcγRIV by a M2e-specific single domain antibody construct protects against influenza A virus infection [PDF]

, 2019
Lower respiratory tract infections, such as infections caused by influenza A viruses, are a constant threat for public health. Antivirals are indispensable to control disease caused by epidemic as well as pandemic influenza A.
Ballegeer, Marlies, Creytens, Sarah, De Vlieger, Dorien, Hengel, Hartmut, Hoffmann, Katja, Nerinckx, Wim, Remaut, Han, Saelens, Xavier, Schepens, Bert, Van Hoecke, Lien, Van Molle, Inge   +10 more
core   +2 more sources

The effects of affinity and valency of an albumin-binding domain (ABD) on the half-life of a single-chain diabody-ABD fusion protein [PDF]

Protein Engineering Design and Selection, 2010
Fusion of small recombinant antibody fragments to an albumin-binding domain (ABD) from streptococcal protein G strongly extends their plasma half-life. This ABD binds with nanomolar affinity to human (HSA) and mouse serum albumin (MSA). It was speculated that an increase in albumin-binding affinity should lead to a further increase in half-life. In the
Jonas, Hopp, Nora, Hornig, Kirstin A, Zettlitz, Aline, Schwarz, Nadine, Fuss, Dafne, Müller, Roland E, Kontermann   +6 more
openaire   +2 more sources