Frontiers in Bioengineering and Biotechnology, 2022 Nanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids.
Marco Pitino +4 more
doaj +1 more source
Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human κ light chain [PDF]
Biochemical Journal, 1999 The placement of a tryptophan residue into a single Ig-binding-domain of protein L from Peptostreptococcus magnus has been used to examine the binding interactions between the binding domain and ĸ light chains (ĸ-chains). The fluorescence intensity of the mutant domain increases on the formation of a complex with ĸ-chains.
J A, Beckingham +4 more
openaire +2 more sources
Mapping of the ligand-binding site on the b ' domain of human PDI: interaction with peptide ligands and the x-linker region [PDF]
, 2009 PDI (protein disulfide-isomerase) catalyses the formation of native disulfide bonds of secretory proteins in the endoplasmic reticulum. PDI consists of four thioredoxin-like domains, of which two contain redox-active catalytic sites (a and a'), and two ...
Freedman, R. B. +15 more
core +1 more source
Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronectin in Pichia pastoris [PDF]
Protein Expression and Purification, 2001 The Pichia pastoris expression system was used to produce functionalized single-chain antibody fragments (scFv) directed against the ED-B domain of the B-fibronectin (B-Fn) isoform which was found to be present only in newly formed blood vessels during tumor angiogenesis.
Marty, C +4 more
openaire +3 more sources
Observation and Characterization of the Interaction between a Single Immunoglobulin Binding Domain of Protein L and Two Equivalents of Human κ Light Chains [PDF]
Journal of Biological Chemistry, 2004 Detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single Ig-binding domain of protein L from Peptostreptococcus magnus binding to kappa light chain are due to the ...
Housden, N.G. +9 more
openaire +4 more sources
Proceedings of the National Academy of Sciences, 1992 We demonstrate that the B chain of ricin toxin preserves its lectin activity when expressed as a fusion protein on the surface of fd phage. Moreover, B chain, which folds into two topologically similar globular domains, can be dissected into amino-terminal and carboxyl-terminal domains to form single binding domains (SBDs) of B chain, each of which ...
C, Swimmer +5 more
openaire +3 more sources
Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear [PDF]
, 2010 Myo1c is one of eight members of the mammalian myosin I family of actin-associated molecular motors. In stereocilia of the hair cells in the inner ear, Myo1c presumably serves as the adaptation motor, which regulates the opening and closing of ...
Adamek, Nancy +3 more
core +1 more source
Bglbrick strategy for the construction of single domain antibody fusions
Heliyon, 2017 Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman +3 more
doaj +1 more source
Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH
eLife, 2022 The human proteome is replete with short linear motifs (SLiMs) of four to six residues that are critical for protein-protein interactions, yet the importance of the sequence surrounding such motifs is underexplored.
Theresa Hwang +7 more
doaj +1 more source
Heavy and Light Chain Variable Single Domains of an Anti-DNA Binding Antibody Hydrolyze Both Double- and Single-stranded DNAs without Sequence Specificity [PDF]
Journal of Biological Chemistry, 2006 Anti-DNA antibodies (Abs) are of biomedical interest because they are associated with autoimmune diseases in human and mice. Previously we isolated an anti-DNA monoclonal Ab 3D8 from an autoimmune-prone MRL-lpr/lpr mouse. Here we have characterized DNA binding kinetics and hydrolyzing activities of the recombinant single chain variable fragment (scFv ...
Young-Rim, Kim +9 more
openaire +2 more sources