Results 11 to 20 of about 507,473 (216)

An Inside Job: Applications of Intracellular Single Domain Antibodies

Biomolecules, 2020
Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is ...
Eline Soetens, Marlies Ballegeer, Xavier Saelens   +2 more
doaj   +1 more source

Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein. [PDF]

PLoS ONE, 2016
Single-domain antibodies derived from the unique New Antigen Receptor found in sharks have numerous potential applications, ranging from diagnostic reagents to therapeutics.
George P Anderson, Daniel D Teichler, Dan Zabetakis, Lisa C Shriver-Lake, Jinny L Liu, Stephen G Lonsdale, Sarah A Goodchild, Ellen R Goldman   +7 more
doaj   +1 more source

The Application of Nanobody in CAR-T Therapy

Biomolecules, 2021
Chimeric antigen receptor (CAR) T therapy represents a form of immune cellular therapy with clinical efficacy and a specific target. A typical chimeric antigen receptor (CAR) construct consists of an antigen binding domain, a transmembrane domain, and a ...
Chaolemeng Bao, Quanli Gao, Lin-Lin Li, Lu Han, Bingxiang Zhang, Yijin Ding, Zongpei Song, Ruining Zhang, Jishuai Zhang, Xian-Hui Wu   +9 more
doaj   +1 more source

Special Issue: Nanobody

Antibodies, 2020
Since their first description in 1993 [1], single-domain antibody fragments derived from heavy-chain-only antibodies of camelids have received increasing attention as highly versatile binding molecules in the fields of biotechnology and medicine [...]
Patrick Chames, Ulrich Rothbauer
doaj   +1 more source

Grabbing the Bull by Both Horns: Bovine Ultralong CDR-H3 Paratopes Enable Engineering of ‘Almost Natural’ Common Light Chain Bispecific Antibodies Suitable For Effector Cell Redirection

Frontiers in Immunology, 2022
A subset of antibodies found in cattle comprises ultralong CDR-H3 regions of up to 70 amino acids. Interestingly, this type of immunoglobulin usually pairs with the single germline VL gene, V30 that is typically very conserved in sequence.
Daniel Klewinghaus, Lukas Pekar, Paul Arras, Simon Krah, Bernhard Valldorf, Harald Kolmar, Stefan Zielonka   +6 more
doaj   +1 more source

Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction [PDF]

PeerJ, 2020
Antigen binding by antibodies requires precise orientation of the complementarity- determining region (CDR) loops in the variable domain to establish the correct contact surface.
Akhila Melarkode Vattekatte, Nicolas Ken Shinada, Tarun J. Narwani, Floriane Noël, Olivier Bertrand, Jean-Philippe Meyniel, Alain Malpertuy, Jean-Christophe Gelly, Frédéric Cadet, Alexandre G. de Brevern   +9 more
doaj   +2 more sources

Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme

Frontiers in Bioengineering and Biotechnology, 2022
Nanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids.
Marco Pitino, Laura A. Fleites, Lauren Shrum, Michelle Heck, Robert G. Shatters   +4 more
doaj   +1 more source

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. [PDF]

PLoS ONE, 2013
Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C.
Ali Riazi, Philippa C R Strong, Russell Coleman, Wangxue Chen, Tomoko Hirama, Henk van Faassen, Matthew Henry, Susan M Logan, Christine M Szymanski, Roger Mackenzie, Mehdi Arbabi Ghahroudi   +10 more
doaj   +1 more source

Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH

eLife, 2022
The human proteome is replete with short linear motifs (SLiMs) of four to six residues that are critical for protein-protein interactions, yet the importance of the sequence surrounding such motifs is underexplored.
Theresa Hwang, Sara S Parker, Samantha M Hill, Robert A Grant, Meucci W Ilunga, Venkatesh Sivaraman, Ghassan Mouneimne, Amy E Keating   +7 more
doaj   +1 more source