Results 1 to 10 of about 478,505 (298)

An activating mutation reveals a second binding mode of the integrin α2 I domain to the GFOGER motif in collagens.

PLoS ONE, 2013
The GFOGER motif in collagens (O denotes hydroxyproline) represents a high-affinity binding site for all collagen-binding integrins. Other GxOGER motifs require integrin activation for maximal binding.
Federico Carafoli, Samir W Hamaia, Dominique Bihan, Erhard Hohenester, Richard W Farndale   +4 more
doaj   +1 more source

The role of the C-domain of bacteriophage T4 gene 32 protein in ssDNA binding and dsDNA helix-destabilization: Kinetic, single-molecule, and cross-linking studies. [PDF]

PLoS ONE, 2018
The model single-stranded DNA binding protein of bacteriophage T4, gene 32 protein (gp32) has well-established roles in DNA replication, recombination, and repair. gp32 is a single-chain polypeptide consisting of three domains.
Kiran Pant, Brian Anderson, Hendrik Perdana, Matthew A Malinowski, Aye T Win, Christopher Pabst, Mark C Williams, Richard L Karpel   +7 more
doaj   +1 more source

Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein

eLife, 2022
As the only major retrograde transporter along microtubules, cytoplasmic dynein plays crucial roles in the intracellular transport of organelles and other cargoes.
Kayla A Jara, Nikolaus M Loening, Patrick N Reardon, Zhen Yu, Prajna Woonnimani, Coban Brooks, Cat H Vesely, Elisar J Barbar   +7 more
doaj   +1 more source

A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. [PDF]

PLoS ONE, 2013
RYK is an unusual member of the receptor tyrosine kinase (RTK) family that is classified as a putative pseudokinase. RYK regulates fundamental biological processes including cell differentiation, migration and target selection, axon outgrowth and ...
Michael M Halford, Maria L Macheda, Clare L Parish, Elena A Takano, Stephen Fox, Daniel Layton, Edouard Nice, Steven A Stacker   +7 more
doaj   +1 more source

In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties to Facilitate Clinical Development

Frontiers in Immunology, 2017
Molecular engineering to increase the percentage identity to common human immunoglobulin sequences of non-human therapeutic antibodies and scaffolds has become standard practice.
John Steven, Mischa R. Müller, Miguel F. Carvalho, Obinna C. Ubah, Marina Kovaleva, Gerard Donohoe, Thomas Baddeley, Dawn Cornock, Kenneth Saunders, Andrew J. Porter, Andrew J. Porter, Caroline Jane Barelle   +11 more
doaj   +1 more source

Generation of robust bispecific antibodies through fusion of single-domain antibodies on IgG scaffolds: a comprehensive comparison of formats

mAbs, 2023
Bispecific antibodies (bsAbs) enable dual binding of different antigens with potential synergistic targeting effects and innovative therapeutic possibilities.
Andreas V. Madsen, Peter Kristensen, Alexander K. Buell, Steffen Goletz   +3 more
doaj   +1 more source

Recombinant rabbit single‐chain antibodies bind to the catalytic and C‐terminal domains of HIV‐1 integrase protein and strongly inhibit HIV‐1 replication [PDF]

Biotechnology and Applied Biochemistry, 2012
AbstractThe human immunodeficiency virus type 1 (HIV‐1) integrase (IN) protein plays an important role during the early stages of the retroviral life cycle and therefore is an attractive target for therapeutic intervention. We immunized rabbits with HIV‐1 IN protein and developed a combinatorial single‐chain variable fragment (scFv) library against IN.
Frederico Aires, da Silva, Min, Li, Sylvie, Rato, Sara, Maia, Rui, Malhó, Kylie, Warren, David, Harrich, Robert, Craigie, Carlos, Barbas, Joao, Goncalves   +9 more
openaire   +2 more sources

Development and evaluation of nanobody tracers for noninvasive nuclear imaging of the immune-checkpoint TIGIT

Frontiers in Immunology, 2023
IntroductionT cell Ig and ITIM domain receptor (TIGIT) is a next-generation immune checkpoint predominantly expressed on activated T cells and NK cells, exhibiting an unfavorable prognostic association with various malignancies.
Katty Zeven, Timo W.M. De Groof, Hannelore Ceuppens, Robin Maximilian Awad, Thomas Ertveldt, Wout de Mey, Fien Meeus, Geert Raes, Geert Raes, Karine Breckpot, Nick Devoogdt   +10 more
doaj   +1 more source

Generation and utility of a single-chain fragment variable monoclonal antibody platform against a baculovirus expressed recombinant receptor binding domain of SARS-CoV-2 spike protein

Molecular Immunology, 2022
As the second wave of COVID-19 launched, various variants of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) have emerged with a dramatic global spread amongst millions of people causing unprecedented case fatalities and economic shut-downs. That initiated a necessity for developing specific diagnostics and therapeutics along with vaccines
Reda Salem, Alaa A. El-Kholy, Fatma R. Waly, Dalia Ayman, Aya Sakr, Mai Hussein   +5 more
openaire   +2 more sources

Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies.

PLoS ONE, 2015
Single domain antibodies are recombinantly expressed functional antibodies devoid of light chains. These binding elements are derived from heavy chain antibodies found in camelids and offer several distinctive properties for applications in biotechnology
Eduardo Antonio Della Pia, Karen L Martinez   +1 more
doaj   +1 more source