Results 21 to 30 of about 852,815 (296)

Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme

Frontiers in Bioengineering and Biotechnology, 2022
Nanobodies® (VHH antibodies), are small peptides that represent the antigen binding domain, VHH of unique single domain antibodies (heavy chain only antibodies, HcAb) derived from camelids.
Marco Pitino, Laura A. Fleites, Lauren Shrum, Michelle Heck, Robert G. Shatters   +4 more
doaj   +1 more source

Interactions between a single immunoglobulin-binding domain of protein L from Peptostreptococcus magnus and a human κ light chain [PDF]

Biochemical Journal, 1999
The placement of a tryptophan residue into a single Ig-binding-domain of protein L from Peptostreptococcus magnus has been used to examine the binding interactions between the binding domain and ĸ light chains (ĸ-chains). The fluorescence intensity of the mutant domain increases on the formation of a complex with ĸ-chains.
J A, Beckingham, S P, Bottomley, R, Hinton, B J, Sutton, M G, Gore   +4 more
openaire   +2 more sources

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

Native proline-rich motifs exploit sequence context to target actin-remodeling Ena/VASP protein ENAH

eLife, 2022
The human proteome is replete with short linear motifs (SLiMs) of four to six residues that are critical for protein-protein interactions, yet the importance of the sequence surrounding such motifs is underexplored.
Theresa Hwang, Sara S Parker, Samantha M Hill, Robert A Grant, Meucci W Ilunga, Venkatesh Sivaraman, Ghassan Mouneimne, Amy E Keating   +7 more
doaj   +1 more source

Phage display of ricin B chain and its single binding domains: system for screening galactose-binding mutants.

Proceedings of the National Academy of Sciences, 1992
We demonstrate that the B chain of ricin toxin preserves its lectin activity when expressed as a fusion protein on the surface of fd phage. Moreover, B chain, which folds into two topologically similar globular domains, can be dissected into amino-terminal and carboxyl-terminal domains to form single binding domains (SBDs) of B chain, each of which ...
C, Swimmer, S M, Lehar, J, McCafferty, D J, Chiswell, W A, Blättler, B C, Guild   +5 more
openaire   +3 more sources

Pentavalent single-domain antibodies reduce Campylobacter jejuni motility and colonization in chickens. [PDF]

PLoS ONE, 2013
Campylobacter jejuni is the leading cause of bacterial foodborne illness in the world, with symptoms ranging from acute diarrhea to severe neurological disorders. Contaminated poultry meat is a major source of C.
Ali Riazi, Philippa C R Strong, Russell Coleman, Wangxue Chen, Tomoko Hirama, Henk van Faassen, Matthew Henry, Susan M Logan, Christine M Szymanski, Roger Mackenzie, Mehdi Arbabi Ghahroudi   +10 more
doaj   +1 more source

A comparison of the binding sites of antibodies and single-domain antibodies

Frontiers in Immunology, 2023
Antibodies are the largest class of biotherapeutics. However, in recent years, single-domain antibodies have gained traction due to their smaller size and comparable binding affinity.
Gemma L. Gordon, Henriette L. Capel, Bora Guloglu, Eve Richardson, Ryan L. Stafford, Charlotte M. Deane   +5 more
doaj   +1 more source

Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire. [PDF]

, 2016
Staphylococcus aureus is both an important pathogen and a human commensal. To explore this ambivalent relationship between host and microbe, we analysed the memory humoral response against IsdB, a protein involved in iron acquisition, in four healthy ...
Abdiche, Yasmina, Chaparro-Riggers, Javier, Deng, Xiaodi, Foletti, Davide, Glanville, Jacob, Hasa-Moreno, Adela, Lindquist, Kevin, Melton Witt, Jody, Ni, Irene, Pham, Amber, Pitts, Steven, Pons, Jaume, Rajpal, Arvind, Shelton, David, Sirota, Marina, Strop, Pavel, Sundar, Purnima D, Yeung, Yik Andy   +17 more
core   +2 more sources

Structure of the Single-lobe Myosin Light Chain C in Complex with the Light Chain-binding Domains of Myosin-1C Provides Insights into Divergent IQ Motif Recognition [PDF]

Journal of Biological Chemistry, 2016
Myosin light chains are key regulators of class 1 myosins and typically comprise two domains, with calmodulin being the archetypal example. They bind IQ motifs within the myosin neck region and amplify conformational changes in the motor domain. A single lobe light chain, myosin light chain C (MlcC), was recently identified and shown to specifically ...
David N, Langelaan, Janine, Liburd, Yidai, Yang, Emily, Miller, Seth, Chitayat, Scott W, Crawley, Graham P, Côté, Steven P, Smith   +7 more
openaire   +2 more sources

An activating mutation reveals a second binding mode of the integrin α2 I domain to the GFOGER motif in collagens.

PLoS ONE, 2013
The GFOGER motif in collagens (O denotes hydroxyproline) represents a high-affinity binding site for all collagen-binding integrins. Other GxOGER motifs require integrin activation for maximal binding.
Federico Carafoli, Samir W Hamaia, Dominique Bihan, Erhard Hohenester, Richard W Farndale   +4 more
doaj   +1 more source