Results 11 to 20 of about 411,726 (313)
Molecular Cell, 2004 An array of gene silencing pathways share a common early step: Dicer cleaves double-stranded RNA (dsRNA) into approximately 20-25 nucleotide fragments that direct the silencing machinery to specific targets. A recent report in Cell reveals how Dicer's two RNase III domains collaborate during dsRNA processing and sets the stage for a deeper ...
Pham, John W, Sontheimer, Erik J
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Filtering of Ineffective siRNAs and Improved siRNA Design Tool [PDF]
Bioinformatics, 2004 Abstract Motivation: Short interfering RNAs (siRNAs) can be used to suppress gene expression and possess many potential applications in therapy, but how to design an effective siRNA is still not clear. Based on the MPI (Max-Planck-Institute) basic principles, a number of siRNA design tools have been developed recently.
Siu-Ming Yiu +6 more
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Targeted Delivery of siRNA [PDF]
BioMed Research International, 2006 Therapeutic application of siRNA requires delivery to the correct intracellular location, to interact with the RNAi machinery within the target cell, within the target tissue responsible for the pathology. Each of these levels of targeting poses a significant barrier.
Oliveira, S. +2 more
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Simple Modifications of Branched PEI Lead to Highly Efficient siRNA Carriers with Low Toxicity [PDF]
, 2008 Polymer carriers like PEI which proved their efficiency in DNA delivery were found to be far less effective for the applications with siRNA. In the current study, we generated a number of nontoxic derivates of branched PEI through modification of amines ...
Zintchenko, Arkadi +3 more
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Novel Cell type-specific aptamer-siRNA delivery system for HIV-1 therapy [PDF]
, 2007 The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires safe and efficient delivery to specific cells and tissues. Here we demonstrate cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. The
Haitang Li +4 more
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RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
, 2013 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Jo Milner +4 more
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Retrovirus-delivered siRNA [PDF]
BMC Biotechnology, 2002 The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high production costs limit this technology's utility for many laboratories and experimental situations.
Devroe, Eric, Silver, Pamela
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An Engineering Approach to Cancer Therapy Using Systemically Delivered siRNA [PDF]
, 2007 The next generation of cancer therapeutics will specifically target processes responsible for the growth and survival of cancer cells. Among the most promising of these molecularly targeted therapeutics are small interfering RNAs (siRNAs). These siRNAs
Bartlett, Derek William
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Mathematical Modeling: A Tool for Optimization of Lipid Nanoparticle-Mediated Delivery of siRNA
Molecular Therapy: Nucleic Acids, 2017 Lipid nanoparticles (LNPs) have been used to successfully deliver small interfering RNAs (siRNAs) to target cells in both preclinical and clinical studies and currently are the leading systems for in vivo delivery. Here, we propose the use of an ordinary
Radu Mihaila +9 more
doaj +1 more source
A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. [PDF]
, 2015 Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research.
Hart, S.L. +27 more
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