Results 21 to 30 of about 535,239 (304)

Functional siRNA Delivery by Extracellular Vesicle–Liposome Hybrid Nanoparticles

Advanced Healthcare Materials, 2021
The therapeutic use of RNA interference is limited by the inability of siRNA molecules to reach their site of action, the cytosol of target cells. Lipid nanoparticles, including liposomes, are commonly employed as siRNA carrier systems to overcome this ...
M. Evers, Simonides I. van de Wakker, Ellis M. de Groot, Olivier G. de Jong, Jerney J. Gitz-Francois, Cor S. Seinen, J. Sluijter, R. Schiffelers, P. Vader   +8 more
semanticscholar   +1 more source

Simple Modifications of Branched PEI Lead to Highly Efficient siRNA Carriers with Low Toxicity [PDF]

, 2008
Polymer carriers like PEI which proved their efficiency in DNA delivery were found to be far less effective for the applications with siRNA. In the current study, we generated a number of nontoxic derivates of branched PEI through modification of amines ...
Zintchenko, Arkadi, Philipp, Alexander, Wagner, Ernst, Dehshahri, Ali   +3 more
core   +1 more source

siRNA-induced liver ApoB knockdown lowers serum LDL-cholesterol in a mouse model with human-like serum lipids[S]

Journal of Lipid Research, 2011
Increased serum apolipoprotein (apo)B and associated LDL levels are well-correlated with an increased risk of coronary disease. ApoE–/– and low density lipoprotein receptor (LDLr)–/– mice have been extensively used for studies of coronary atherosclerosis.
Marija Tadin-Strapps, Laurence B. Peterson, Anne-Marie Cumiskey, Raymond L. Rosa, Vivienne Halili Mendoza, Jose Castro-Perez, Oscar Puig, Liwen Zhang, Walter R. Strapps, Satyasri Yendluri, Lori Andrews, Victoria Pickering, Julie Rice, Lily Luo, Zhu Chen, Samnang Tep, Brandon Ason, Elizabeth Polizzi Somers, Alan B. Sachs, Steven R. Bartz, Jenny Tian, Jayne Chin, Brian K. Hubbard, Kenny K. Wong, Lyndon J. Mitnaul   +24 more
doaj   +1 more source

Retrovirus-delivered siRNA [PDF]

BMC Biotechnology, 2002
The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high production costs limit this technology's utility for many laboratories and experimental situations.
Devroe, Eric, Silver, Pamela
openaire   +5 more sources

Exosomes are natural carriers of exogenous siRNA to human cells in vitro [PDF]

, 2013
© 2013 Shtam et al. Background: Exosomes are nano-sized vesicles of endocytic origin that are involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA.
Varfolomeeva, EY, Kovalev, RA, Makarov, EM, Kil, YV, Filatov, MV, Shtam, TA   +5 more
core   +1 more source

A SARS-CoV-2 targeted siRNA-nanoparticle therapy for COVID-19

Molecular Therapy, 2021
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in humans. Despite several emerging vaccines, there remains no verifiable therapeutic targeted specifically to the virus.
A. Idris, Alicia Davis, Aroon Supramaniam, D. Acharya, Gabriella Kelly, Yaman Tayyar, N. West, Ping Zhang, Christopher L. D. McMillan, Citradewi Soemardy, Roslyn M. Ray, D. O’Meally, Tristan A. Scott, N. McMillan, Kevin V. Morris   +14 more
semanticscholar   +1 more source

siRNA-optimized Modifications for Enhanced In Vivo Activity

Molecular Therapy: Nucleic Acids, 2012
Current modifications used in small interfering RNAs (siRNAs), such as 2’-methoxy (2’-OMe) and 2’-fluoro (2’-F), improve stability, specificity or immunogenic properties but do not improve potency.
Denise M Kenski, Gabor Butora, Aarron T Willingham, Abby J Cooper, Wenlang Fu, Ning Qi, Ferdie Soriano, Ian W Davies, W Michael Flanagan   +8 more
doaj   +1 more source

Role of non-coding RNAs in Dengue virus-host interaction

Frontiers in Bioscience-Scholar, 2021
Dengue is potentially a life-threatening arthropod-borne viral infection for which there are no known therapeutic agents till date. Early stage diagnosis of dengue infection is still lacking.
Deeksha Madhry, Kush Kumar Pandey, Jaskaran Kaur, Yogita Rawat, Leena Sapra, Ravi Kumar Y.S., Rupesh K. Srivastava, Sankar Bhattacharyya, Bhupendra Verma   +8 more
doaj   +1 more source

A method for concentrating lipid peptide DNA and siRNA nanocomplexes that retains their structure and transfection efficiency. [PDF]

, 2015
Nonviral gene and small interfering RNA (siRNA) delivery formulations are extensively used for biological and therapeutic research in cell culture experiments, but less so in in vivo and clinical research.
Hart, S.L., Zhou, Haiyan, Munye, Mustafa M, Hart, Stephen L, Munye, MM, Munye MM, Tagalakis AD, Tagalakis, Aristides D, Tagalakis, Aristides, White, Edward A, White, EA, Castellaro, S, Zhou, H, Zhou H, Castellaro S, White EA, Bienemann, A, White, E.A., McCarthy, David, Munye, M.M., Castellaro, Sara, Tagalakis, AD, Hart, SL, Bienemann, Ali S, McCarthy, D, Bienemann A, Hart SL, McCarthy D   +27 more
core   +1 more source

siRNA Specificity: RNAi Mechanisms and Strategies to Reduce Off-Target Effects

Frontiers in Plant Science, 2021
Short interfering RNAs (siRNAs) are processed from long double-stranded RNA (dsRNA), and a guide strand is selected and incorporated into the RNA-induced silencing complex (RISC).
Julia Neumeier, G. Meister
semanticscholar   +1 more source