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Nanomedicine, 2013
By nature, cancer cells are the toughest cells against any treatment. The various available therapies would be much more effective at much lower dose if siRNA against the therapeutic resistance could be identified and successfully codelivered by nanocarriers such as polymer conjugation, micelles, liposomes, solid lipid nanoparticles, nanohydrogels and ...
Zhao, J., Mi, Y., Feng, S.-S.
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By nature, cancer cells are the toughest cells against any treatment. The various available therapies would be much more effective at much lower dose if siRNA against the therapeutic resistance could be identified and successfully codelivered by nanocarriers such as polymer conjugation, micelles, liposomes, solid lipid nanoparticles, nanohydrogels and ...
Zhao, J., Mi, Y., Feng, S.-S.
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Chemical Modification of siRNA
Current Protocols in Nucleic Acid Chemistry, 2009AbstractThe ability to manipulate the RNA interference (RNAi) machinery to specifically silence the expression of target genes could be a powerful therapeutic strategy. Since the discovery that RNAi can be triggered in mammalian cells by short double‐stranded RNAs (small interfering RNA, siRNA), there has been a tremendous push by researchers, from ...
Deleavey, Glen F. +2 more
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Trends in Pharmacological Sciences, 2004
The ability of small-interfering RNAs (siRNAs) to silence gene expression in somatic mammalian cells has provided researchers with a novel tool to block the expression of disease-causing genes, provided that their mRNA sequences are known. siRNAs can be delivered to cells either exogenously as synthetic agents or endogenously as gene-encoding siRNAs ...
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The ability of small-interfering RNAs (siRNAs) to silence gene expression in somatic mammalian cells has provided researchers with a novel tool to block the expression of disease-causing genes, provided that their mRNA sequences are known. siRNAs can be delivered to cells either exogenously as synthetic agents or endogenously as gene-encoding siRNAs ...
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Molecular and Cellular Endocrinology, 2004
RNA interference (RNAi) represents a mechanism invented by nature to protect the genome. In the past few years the field has emerged at a surprisingly high pace. The underlying molecular mechanism of gene silencing provides us with short interfering RNAs (siRNAs) which allows to target any gene with high specificity and efficiency.
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RNA interference (RNAi) represents a mechanism invented by nature to protect the genome. In the past few years the field has emerged at a surprisingly high pace. The underlying molecular mechanism of gene silencing provides us with short interfering RNAs (siRNAs) which allows to target any gene with high specificity and efficiency.
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2021
Exosomes are membrane-bound vesicles (40-100 nm) of endocytic origin released by numerous cell types that act as natural carriers of mRNA, microRNA, and proteins between cells. We developed a new system that uses intravenous injection of modified exosomes for siRNA delivery into the brain.
Lydia Alvarez-Erviti, María Izco
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Exosomes are membrane-bound vesicles (40-100 nm) of endocytic origin released by numerous cell types that act as natural carriers of mRNA, microRNA, and proteins between cells. We developed a new system that uses intravenous injection of modified exosomes for siRNA delivery into the brain.
Lydia Alvarez-Erviti, María Izco
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Cold Spring Harbor Protocols, 2019
This protocol describes how to anneal synthetic sense and antisense siRNAs to form siRNA duplexes, as well as the analysis of siRNA duplexes using nondenaturing polyacrylamide gel electrophoresis.
Phillip D. Zamore, Chengjian Li
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This protocol describes how to anneal synthetic sense and antisense siRNAs to form siRNA duplexes, as well as the analysis of siRNA duplexes using nondenaturing polyacrylamide gel electrophoresis.
Phillip D. Zamore, Chengjian Li
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Oligonucleotides, 2009
Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. Synthetic siRNA in delivery vehicles that facilitate cellular uptake can induce high levels of inflammatory cytokines and
Adam Judge +2 more
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Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. Synthetic siRNA in delivery vehicles that facilitate cellular uptake can induce high levels of inflammatory cytokines and
Adam Judge +2 more
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siRNA and Cancer Immunotherapy
Immunotherapy, 2012Immunotherapeutic approaches have been gaining attention in the field of cancer treatment because of their possible ability to eradicate cancer cells as well as metastases by recruiting the host immune system. On the other hand, RNA-based therapeutics with the ability to silence expression of specific targets are currently under clinical investigation ...
Soudeh Ghafouri-Fard +1 more
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Cell type–specific delivery of siRNAs with aptamer-siRNA chimeras [PDF]
Nature Biotechnology, 2006 Technologies that mediate targeted delivery of small interfering RNAs (siRNAs) are needed to improve their therapeutic efficacy and safety. Therefore, we have developed aptamer-siRNA chimeric RNAs capable of cell type-specific binding and delivery of functional siRNAs into cells.
Paloma H. Giangrande +7 more
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Imaging of Electrotransferred siRNA
2016SiRNA delivery to the cytoplasm can be obtained through the application of calibrated electric field pulses to a mixture of cells and oligonucleotides. To investigate the uptake pathway, time lapse confocal fluorescence microscopy provides a direct visualization of the transfer.
Justin Teissié +3 more
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