Results 151 to 160 of about 347,258 (205)

Site-Directed Mutagenesis of VvCYP76F14 (Cytochrome P450) Unveils Its Potential for Selection in Wine Grape Varieties Linked to the Development of Wine Bouquet. [PDF]

J Agric Food Chem
Peng B, Ran J, Li Y, Tang M, Xiao H, Shi S, Ning Y, Dark A, Li J, Guan X, Song Z.   +10 more
europepmc   +1 more source

The role of dinB in UV survival and UV-induced mutagenesis in Escherichia coli [PDF]

, 2009
Lee, Mei-Chong Wendy
core   +1 more source

Site-directed mutagenesis of tissue factor pathway inhibitor-binding exosite D60A on factor VII results in a new factor VII variant with lower coagulant activity. [PDF]

Res Pract Thromb Haemost
Seanoon K, Kitiyanant V, Payongsri P, Sirachainan N, Angchaisuksiri P, Chuansumrit A, Hongeng S, Tanratana P.   +7 more
europepmc   +1 more source

Primary charge separation in the reaction centers of Rhodobacter sphaeroides [PDF]

, 1990
Baltschewsky, M., Finkele, Ulrich, Holzapfel, Wolfgang, Kaiser, Wolfgang, Oesterhelt, Dieter, Scheer, Hugo, Stilz, Hans Ulrich, Zinth, Wolfgang   +7 more
core  

Deregulation of cellulose synthesis by site-directed mutagenesis of cellulose synthase leads to heightened bacterial cellulose production

Upton DJ, Eastmond H, Gatenby A, Lanot A, Bruce NC.   +4 more
europepmc   +1 more source

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Site-Directed Mutagenesis

2010
The technique of site-directed mutagenesis has been used to characterize gene and protein structure-function relationships, protein-protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The
Carrigan, Patricia E., Baller, Petek, Tuzmen, Sukru   +2 more
openaire   +2 more sources

Site-Directed Mutagenesis

2013
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
openaire   +3 more sources

PCR-Mediated Site-Directed Mutagenesis

Cold Spring Harbor Protocols, 2013
Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins.
Michael F, Carey, Craig L, Peterson, Stephen T, Smale   +2 more
openaire   +2 more sources

Site-directed mutagenesis

Trends in Plant Science, 2000
A system has been developed for site-directed mutagenesis in plants. It has been dubbed the pinpoint technique because it takes advantage of the plant’s DNA repair system and makes use of specifically designed hairpin-shaped molecules of DNA and RNA.
openaire   +1 more source