Results 151 to 160 of about 354,238 (282)

Molecular modelling and site-directed mutagenesis provide insight into saccharide pyruvylation by the Paenibacillus alvei CsaB enzyme. [PDF]

Sci Rep, 2023
Stefanović C, Hager-Mair FF, Breslmayr E, López-Guzmán A, Lim C, Blaukopf M, Kosma P, Oostenbrink C, Ludwig R, Schäffer C.   +9 more
europepmc   +1 more source

Nanoparticle Immunoadjuvant Complexes Augment Germinal Center Responses to Vaccination

Advanced Science, EarlyView.
Scaffolding IL‐21 on the surface of a self‐assembling nanoparticle immunogen drives improved germinal center and humoral immune responses. These nanoparticle immunoadjuvant complexes (NICs) functionally modulate the germinal center driving improved somatic hypermutation and antibody maturation, suggesting this platform has potential utility as a ...
Nicholas J. Tursi, Colby J. Agostino, Jinwei Huang, Toshitha Kannan, Niklas Laenger, Jennifer Londregan, Katlyn Lederer, Michaela Helble, Nicole Bedanova, Cory Livingston, Ebony N. Gary, Madison McCanna, Marta Tarquis Medina, Rumi Habib, Ignacio Rodriguez Relaño, Ivan Maillard, Ami Patel, David Allman, Andrew Kossenkov, Amelia Escolano, Daniel W. Kulp, David. B. Weiner   +21 more
wiley   +1 more source

Site-directed mutagenesis improves the practical application of L-glutamic acid decarboxylase in Escherichia coli. [PDF]

Eng Life Sci, 2023
Fengmin L, Heng Z, Xiangjun Z, Xiaobo W, Huiyan L, Haitian F.   +5 more
europepmc   +1 more source

Rapid PCR site-directed mutagenesis. [PDF]

Genome Research, 1994
Gina L, Costa, Michael P, Weiner
openaire   +3 more sources

Structure‐Guided Engineering of a Promiscuous O‐Methyltransferase for a SAM Regeneration Biocatalysis Platform of Methylated Pharmaceuticals

Advanced Science, EarlyView.
A substrate promiscuous and regioselective O‐methyltransferase, SmOMT, is functionally and structurally characterized. A double mutant, SmOMTE152A/I306A, exhibited enhanced catalytic activity. By coupling this mutant with a mutant halide methyltransferase, AtHMTV140T, for SAM regeneration, a superior artificial fusion enzyme, AtHMTV140T‐L95‐SmOMTE152A ...
Xiran Xiong, Jun Song, Shihan Li, Lu Jin, Qi He, Baohui Zhang, Yan Cao, Shanyong Yi, Yanfang Yang, Xiang Li, Juan Li, Wei Huang   +11 more
wiley   +1 more source

Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations. [PDF]

Sci Rep, 2023
Otum CC, Rivière E, Barnard M, Loubser J, Williams MJ, Streicher EM, Van Rie A, Warren RM, Klopper M.   +8 more
europepmc   +1 more source

Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome

Advanced Science, EarlyView.
This study reveals a pivotal epigenetic regulatory role for the histone acetyltransferase p300, demonstrating that its acetylation of histone 3 at lysine 18 and 27 (H3K18ac and H3K27ac), alongside the formation of the p300/BRD4/SP1 complex, drives AR activation and ovarian fibrosis in PCOS.
Zhengquan Zhu, Yihan Wang, Haiyun Chen, Xinye Yu, Tingyu Wang, Yajing Weng, Meihong Guo, Ying Huang, Gaojian Tao, Wangsen Cao, Yong Wang, Daojuan Wang   +11 more
wiley   +1 more source

In-vitro Site-directed Mutagenesis of Human Dihydropteridine Reductase

Pteridines, 1996
Armarego Wilfred L. F., Zhang Hong-Ping, Yang Nan   +2 more
doaj   +1 more source

Cloning independent site-directed mutagenesis using total RNA as template

BioTechniques, 2004
Zsolt B. Nagy, Liliána Z. Fehér, László G. Puskás   +2 more
doaj   +1 more source

Efficiency and Fidelity of Site-Directed Mutagenesis with Complementary Primer Pairs. [PDF]

Cells
Varela-Castillo P, Razavi A, Mousavi N, Robinson N, Yang XJ.   +4 more
europepmc   +1 more source