Results 171 to 180 of about 247,239 (299)

Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis. [PDF]

Front Bioeng Biotechnol, 2023
Xing H, Wang P, Yan X, Yang Y, Li X, Liu R, Zhou Z.   +6 more
europepmc   +1 more source

CXCL10/SLC11A1 Axis Exacerbates Septic Liver Injury by Regulating Neutrophil Extracellular Traps Formation to Drive Macrophage Pro‑Inflammatory Polarization

Advanced Science, EarlyView.
In sepsis‐induced liver injury, marked neutrophil infiltration correlates with disease severity. Neutrophil SLC11A1 drives intracellular Fe2+ and ROS via the Fenton reaction, promoting NETs formation. Hepatocyte‐derived CXCL10 recruits neutrophils and activates the JAK/STAT1/SLC11A1 axis, enhancing NETs‐mediated inflammation.
Haiping Lin, Shicong Zheng, Pei Song, Jie Chang, Zewei Chen, Cang Li, Min Yu, Lude Wang, Shian Yu   +8 more
wiley   +1 more source

Investigating the Deoxyribonuclease Activity of CRM197 with Site-Directed Mutagenesis

ACS Omega, 2019
Nathalie Bravo-Bautista, Hieu Hoang, Anusha Joshi, Jennifer Travis, Melissa Wooten, Nathan J. Wymer   +5 more
doaj   +1 more source

Efficient Site-Directed Mutagenesis Mediated by Primer Pairs with 3'-Overhangs. [PDF]

Curr Protoc
Mousavi N, Zhou E, Razavi A, Ebrahimi E, Varela-Castillo P, Yang XJ.   +5 more
europepmc   +1 more source

Effects of Site-Directed Mutagenesis of Cysteine on the Structure of Sip Proteins. [PDF]

Front Microbiol, 2022
Wang L, Ding MY, Wang J, Gao JG, Liu RM, Li HT.   +5 more
europepmc   +1 more source

Rapid PCR site-directed mutagenesis. [PDF]

Genome Research, 1994
Gina L, Costa, Michael P, Weiner
openaire   +3 more sources

PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer

Advanced Science, EarlyView.
USP10 binds to and stabilizes PFKFB4, enhancing glycolytic ATP production, which activates the urea cycle and elevates fumarate. This inhibits histone demethylase KDM1A, leading to increased H3K4me1 enrichment at the Rad51 promoter and direct activation of Rad51 transcription, which confers lung cancer radioresistance. The PFKFB4 inhibitor 5MPN targets
Yunshang Chen, Zilong Wu, Yongqiang Yang, Ruoxin Fang, Huichan Xue, Rui Zhou, Gang Wu, Xiaohua Jie   +7 more
wiley   +1 more source

Improving enzymatic properties of BlTDH from <i>Bacillus licheniformis</i> through site-directed mutagenesis. [PDF]

Food Chem (Oxf)
Liu X, Gu H, Li H, Chen S, Tang Z, Quan W.   +5 more
europepmc   +1 more source

Molecular Docking and Site-Directed Mutagenesis of GH49 Family Dextranase for the Preparation of High-Degree Polymerization Isomaltooligosaccharide. [PDF]

Biomolecules, 2023
Wang H, Lin Q, Liu M, Ding W, Weng N, Ni H, Lu J, Lyu M, Wang S.   +8 more
europepmc   +1 more source

Modulation of Lung Adenocarcinoma by Phosphorylated FOXN3‐Mediated Transcriptional Inactivation of p53

Advanced Science, EarlyView.
In non‐tumorous lung tissues, FOXN3 promotes the transcriptional activation of p53 by facilitating its recruitment to target promoters, thereby suppressing lung tumorigenesis through activation of the p53 signaling pathway. Conversely, in lung adenocarcinoma tissues, hyperphosphorylated FOXN3 dissociates from the promoters of p53‐responsive genes and ...
Jinjin Yu, Jingyu Yu, Suhui Wang, Xinghong Hu, Lele Jin, Nan Wu, Yuhan Ni, Xueying Zhang, Chengling Zhao, Jihong Zhou, Yong Zhang, Baofa Sun, Hongxing Zhang, Chunfu Zheng, Song Guo Zheng, Wei Li, Xinxing Zhu   +16 more
wiley   +1 more source