Results 151 to 160 of about 349,214 (297)

Molecular modelling and site-directed mutagenesis provide insight into saccharide pyruvylation by the Paenibacillus alvei CsaB enzyme. [PDF]

Sci Rep, 2023
Stefanović C, Hager-Mair FF, Breslmayr E, López-Guzmán A, Lim C, Blaukopf M, Kosma P, Oostenbrink C, Ludwig R, Schäffer C.   +9 more
europepmc   +1 more source

Epithelial TRIM27 Inhibits Intestinal Inflammation in Ulcerative Colitis by the USP7/TRIM27‐IKK Double Negative‐Feedback

Advanced Science, EarlyView.
ABSTRACT The E3 ubiquitin ligase tripartite motif 27 (TRIM27) is a negative regulator of NF‐κB activation and the innate immune response, and TRIM27 deficiency significantly impairs dextran sulfate sodium (DSS)‐induced colitis. The function of TRIM27 in intestinal epithelial cells (IECs), the mechanism by which TRIM27 inhibits the NF‐κB pathway and its
Weimin Xu, Zhebin Hua, Zhujiang Dai, Shasha Zhang, Yihan Jiang, Wensong Ge, YingWei Chen, Zhongchuan Wang, Bing Zhang, Chen‐Ying Liu, Peng Du   +10 more
wiley   +1 more source

Efficiency and Fidelity of Site-Directed Mutagenesis with Complementary Primer Pairs. [PDF]

Cells
Varela-Castillo P, Razavi A, Mousavi N, Robinson N, Yang XJ.   +4 more
europepmc   +1 more source

Site-directed mutagenesis improves the practical application of L-glutamic acid decarboxylase in Escherichia coli. [PDF]

Eng Life Sci, 2023
Fengmin L, Heng Z, Xiangjun Z, Xiaobo W, Huiyan L, Haitian F.   +5 more
europepmc   +1 more source

In-vitro Site-directed Mutagenesis of Human Dihydropteridine Reductase

Pteridines, 1996
Armarego Wilfred L. F., Zhang Hong-Ping, Yang Nan   +2 more
doaj   +1 more source

Cloning independent site-directed mutagenesis using total RNA as template

BioTechniques, 2004
Zsolt B. Nagy, Liliána Z. Fehér, László G. Puskás   +2 more
doaj   +1 more source

POU2F1 Promotes Chemoresistance in Colorectal Cancer Cells via Attenuates the MDR2 Degradation Mediated by PPP1R11 Lactylation

Advanced Science, EarlyView.
Schematic diagram of our study showing that highly expressed POU2F1 in colorectal cancer (CRC) can directly accelerate the cytosolic lactate export to decrease PPP1R11 lactylation at K59, thereby attenuating the E3 ligase enzyme activity and protein stability of PPP1R11, which inhibits the ubiquitination of MDR2 at K413 and K538 but increases the ...
Longzheng Xia, Jinguan Lin, Xianjie Jiang, Linda Oyang, Shiming Tan, Zongyao Ren, Qiu Peng, Qianjin Liao, Yujuan Zhou   +8 more
wiley   +1 more source

Seamless and Highly Efficient Site-directed Mutagenesis for Protein, RNA, and Plasmid Engineering. [PDF]

Curr Protoc
Yang XJ.
europepmc   +1 more source

Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations. [PDF]

Sci Rep, 2023
Otum CC, Rivière E, Barnard M, Loubser J, Williams MJ, Streicher EM, Van Rie A, Warren RM, Klopper M.   +8 more
europepmc   +1 more source

ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid‐β Pathology

Advanced Science, EarlyView.
This study identifies that ALKBH3‐driven m1A demethylation orchestrates Alzheimer's disease progression by disrupting mitochondrial and synaptic homeostasis. This epitranscriptomic mechanism suppresses PINK1‐mediated mitophagy via m1A erasure, leading to mitochondrial dysfunction, oxidative stress, elevated Aβ production, and impaired microglial ...
Yueyang Li, Sifei Yu, Kaidong Lu, Yujie Zhang, Mingjie Dong, Yan Peng, Liang Xue, Waleed Alam, Yuxuan Shui, Yi Zhou, Wuyunhan Ma, Meng Bao, Peiming Li, Peiyi Luo, Tiezhan Lu, Jiajia Li, Kang Zhang, Yuying Wang, Shuchen Yang, Nuoya Yin, Francesco Faiola, Zilong Gao, Jingfeng Zhou, Fei Zhao, Yali He, Magdalena J. Koziol   +25 more
wiley   +1 more source