Results 161 to 170 of about 247,239 (299)

Correction: P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs. [PDF]

J Biol Chem
Mousavi N, Zhou E, Razavi A, Ebrahimi E, Varela-Castillo P, Yang XJ.   +5 more
europepmc   +1 more source

Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis. [PDF]

Biomolecules, 2023
Pandey S, Raut KK, Clark AM, Baudin A, Djemri L, Libich DS, Ponniah K, Pascal SM.   +7 more
europepmc   +1 more source

Computation‐Guided Tunnel Engineering Enhances O2 Transport and H2O2 Resistance of Fe(II)/α‐Ketoglutarate‐Dependent Dioxygenases

Advanced Science, EarlyView.
This study presents a computational tunnel engineering strategy in Fe(II)/α‐ketoglutarate‐dependent dioxygenases (αKGDs) to enhance oxygen (O2) transport, an essential molecule for αKGDs, and limit hydrogen peroxide (H2O2) entry, an inhibitor from the αKG supply system.
Huan Liu, Lunjie Wu, Liying Mao, Songyin Zhao, Hu Liu, Shiyi Xin, Donglin Zhao, Jie Gu, Yan Xu, Xinye Wang, Yao Nie   +10 more
wiley   +1 more source

Site-Directed Mutagenesis of the G4DFsc Protein [PDF]

G4DFsc is a de novo protein designed to mimic the metal-binding active sites of natural non-heme diiron enzymes. Through site-directed mutagenesis, specific amino acids can be targeted to affect conformational changes in the active site.
Kyriakos, Maya
core  

Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis. [PDF]

Biomolecules
Liu S, Yang X, Gao R, Xie G.
europepmc   +1 more source

Improving the Thermostability of Serine Protease PB92 from Bacillus alcalophilus via Site-Directed Mutagenesis Based on Semi-Rational Design. [PDF]

Foods, 2023
Miao H, Xiang X, Han N, Wu Q, Huang Z.
europepmc   +1 more source

Porcine Deltacoronavirus M Protein Binds NLRP3 to Promote Inflammasome Assembly via Competition with TRIM31

Advanced Science, EarlyView.
Porcine deltacoronavirus (PDCoV) infection induces severe intestinal inflammation and acute diarrhea in piglets, yet the molecular mechanism remains incompletely understood. The M protein activates NLRP3 inflammasome through dual mechanisms: direct binding to the NLRP3 LRR domain and disruption of TRIM31‐mediated K48‐linked ubiquitination.
Jinhui Hou, Fangfang Han, Anqi Liu, Yang Yu, Rui Zhao, Zhengting Shi, Yanrong Lv, Jinli Liu, Shaopo Zu, Zhanyong Wei, Jin Yuan, Hui Hu   +11 more
wiley   +1 more source

Amenability of the Gatekeeper Enzyme HphA to Engineering in the Homologation Pathway of l‑Phenylalanine and l‑Tyrosine through Homology-Based Site-Directed Mutagenesis. [PDF]

ACS Omega
Lang Harman RM, Blackstone HG, Aruna FO, Patel SR, Shin M, NeSmith RK, Dickson DB, Spencer AC, Mori S.   +8 more
europepmc   +1 more source

ANXA2+ Small Extracellular Vesicles Drive Chemoresistance in Anaplastic Thyroid Cancer by Promoting XRCC5 Lactylation and Enhancing Non‐Homologous End‐Joining Repair

Advanced Science, EarlyView.
ANXA2+ sEVs promote cisplatin resistance in ATC by stabilizing the SRC/LDHA interaction, increasing LDHA phosphorylation and activity, and lactate production. Elevated lactate levels promote KAT5‐mediated lactylation of XRCC5 at lysine 265, enhancing its binding to XRCC6.
Shanshan Su, YiShan Xiong, Yuxuan Liang, Xiang Min, Daofeng Dai   +4 more
wiley   +1 more source

Engineering the Bacterial Laccase CotA for Functional Expression and Dye Decolorization Through Site-Directed Mutagenesis. [PDF]

Biology (Basel)
Zhou Z, Yao S, Ying S, Yu M, Song Z, Sun Y, Qian L, Zhang Y.   +7 more
europepmc   +1 more source