Advanced Science, EarlyView.ABSTRACT Despite the transformative impact of cancer immunotherapies such as immune checkpoint blockade, durable clinical responses remain limited. Increasing evidence indicates that antitumor immunity is governed not only by the tumor microenvironment, but also by systemic immune regulation mediated by peripheral immune organs. Among these, the spleen
Yuehua Liu, Xiaoqian Nie, Xiaofei Gao
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P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs. [PDF]
J Biol ChemMousavi N +5 more
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My remembrances of H.G. Khorana: exploring the mechanism of bacteriorhodopsin with site-directed mutagenesis and FTIR difference spectroscopy. [PDF]
Biophys Rev, 2023 Rothschild KJ.
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Plant‐Derived Melatonin Inhibits Bacterial Virulence via CpxA/R Two‐Component System
Advanced Science, EarlyView.Plant‐derived melatonin is sensed by CpxAE48/T51, which inhibits the phosphorylation cascade transmission from CpxAH240 to CpxRD52, resulting in the inhibition of DNA‐binding capacity of CpxR and subsequent T3SS genes expression in Pst DC3000. ABSTRACT In defending against pathogens, plants deploy diverse secondary metabolites and signaling molecules ...
Jin‐Wei Wei +7 more
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PCR-Free Site-Directed Mutagenesis on Repetitive Sequences Using Single-Stranded DNA-Assisted Double-Stranded DNA Nicking by DNAzymes. [PDF]
Angew Chem Int Ed EnglLyu M, Kong L, Shao X, Lu Y.
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Site-directed mutagenesis of HLA molecules reveals the functional epitope of a human HLA-A1/A36-specific monoclonal antibody. [PDF]
HLA, 2023 Meng T +15 more
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Programmable RNA N6,2´‐O‐Dimethyladenosine Editing
Advanced Science, EarlyView.ABSTRACT N6,2’‐O‐dimethyladenosine (m6Am) is a prevalent RNA modification located at the first transcribed nucleotide adjacent to the 5′ cap of mRNAs, where it has been implicated in gene regulation. However, the lack of methods for precise, transcript‐specific manipulation of m6Am has limited its functional dissection.
Yang Li +9 more
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Expanding the bioanalytical application of β-hydroxybutyrate binding proteins through characterization of their metabolite interactions and site-directed mutagenesis. [PDF]
Protein SciKane BJ, Murphy KV, Sode K.
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Site-directed mutagenesis identified the key active site residues of 2,3-oxidosqualene cyclase HcOSC6 responsible for cucurbitacins biosynthesis in Hemsleya chinensis. [PDF]
Front Plant Sci, 2023 Li X +11 more
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