Results 201 to 210 of about 349,214 (297)

Critical structural elements for the antigenicity of wheat allergen LTP1 (Tri a 14) revealed by site-directed mutagenesis. [PDF]

Sci Rep, 2022
Mameri H, Gaudin JC, Lollier V, Tranquet O, Brossard C, Pietri M, Marion D, Codreanu-Morel F, Beaudouin E, Wien F, Gohon Y, Briozzo P, Denery-Papini S.   +12 more
europepmc   +1 more source

The Landscape and Regulation of Histone Crotonylation in Mammalian Gametes and Early Embryos

Advanced Science, EarlyView.
Histone crotonylation undergoes a genome‐wide transition from broad domains to canonical narrow peaks during minor zygotic genome activation (ZGA). This remodeling is required for proper major ZGA and blastocyst formation. Disruption of this transition by transcriptional inhibition, metabolic perturbation, or HDAC1 dysfunction impairs embryonic ...
Shenli Yuan, Yelian Yan, Yalin Liang, Kanghua Zhong, Zhican Fu, Xiaobo Wang, Chao Liu, Tao Huang, Keliang Wu   +8 more
wiley   +1 more source

Site-Directed Mutagenesis [PDF]

, 2008
openaire   +2 more sources

Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis. [PDF]

Sci Rep
Paclikova P, Harnos J.
europepmc   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Site-Directed Mutagenesis Increased the Catalytic Activity and Stability of <i>Oenococcus oeni</i> β-Glucosidase: Characterization of Enzymatic Properties and Exploration of Mechanisms. [PDF]

Int J Mol Sci
Zuo J, Zhang J, Ma H, Zhang Y, Li P, Wu Y, Tian P, Fan Q, Cao L, Sun J, Gu S.   +10 more
europepmc   +1 more source

Fosl2 Regulates FSH‐Dependent Follicle Maturation Through Feedback Amplification of FSH/FSHR Signaling

Advanced Science, EarlyView.
This study identifies a FOSL2‐driven positive feedback loop that amplifies FSH/FSHR signaling. During FSH‐dependent follicle maturation, FSH induces Fosl2 expression via the cAMP‐PKA‐CREB cascade. FOSL2 in turn binds the promoters of Fshr and estrogen‐biosynthesis genes to enhance their transcription, thereby increasing Fshr mRNA level and amplifying ...
Hongru Shi, Chaoli Chen, Zaohong Ran, Jianning Liao, Zian Wu, Xiaodong Wang, Yongheng Zhao, Wenkai Ke, Bowen Tan, Yun Liu, Youqiang Su, Wei Ren, Xiang Li, Changjiu He   +13 more
wiley   +1 more source

Correction for Gier et al., "Analysis of Yeast Killer Toxin K1 Precursor Processing via Site-Directed Mutagenesis: Implications for Toxicity and Immunity". [PDF]

mSphere
Gier S, Schmitt MJ, Breinig F.
europepmc   +1 more source

Site-Directed Mutagenesis v1

This protocols describes the procedure for site-directed mutagenesis by PCR
openaire   +1 more source

Microbial Odorant Detection Guides Drosophila Parasitoids Seeking Hosts in Fermenting Fruits

Advanced Science, EarlyView.
Yeast microbes in fermenting fruits attract both host flies and their parasitoid wasps. Female Leptopilina boulardi detect yeast‐emitted ethyl esters via two olfactory receptors, LbouOR167 and LbouOR136. A conserved residue, Leu159, is critical for binding these compounds, enabling female wasps to locate host‐rich habitats.
Yueqi Lu, Lan Pang, Wenqi Shi, Ting Feng, Zhi Dong, Yifeng Sheng, Sicong Zhou, Longtao Yu, Hao Guo, Ying Wang, Jiani Chen, Jianhua Huang   +11 more
wiley   +1 more source