Results 191 to 200 of about 349,214 (297)

OsGSK2‐OsTCP19 Module Integrates Nitrogen and Brassinosteroid Signaling to Regulate Nitrogen Utilization and Root Growth in Rice

Advanced Science, EarlyView.
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu, Weiwei Li, Xiaohui Ma, Xiaohan Wang, Yarong Shi, Xiahe Huang, Xinran Li, Yingchun Wang, Hongning Tong, Bin Hu, Wenbin Zhou, Chengcai Chu   +11 more
wiley   +1 more source

Characterization and structural analysis of a leucine aminopeptidase using site-directed mutagenesis. [PDF]

AMB Express
Men Y, Liu Y, Yin D, Wang G, Qin R, Xiong H, Wang Y.   +6 more
europepmc   +1 more source

Optimization of Signal Peptide via Site-Directed Mutagenesis for Enhanced Secretion of Heterologous Proteins in Lactococcus lactis. [PDF]

Int J Mol Sci, 2022
Alias NAR, Song AA, Alitheen NB, Rahim RA, Othman SS, In LLA.   +5 more
europepmc   +1 more source

Pyroptosis‐Inducing Engineered Microparticles for Cancer Immunotherapy

Advanced Science, EarlyView.
Engineered microparticles co‐delivering geldanamycin and dual nanobodies induce targeted pyroptosis and block PD‐L1 and CD47 pathways, reprogramming the tumor microenvironment and achieving potent antitumor immunity in lung cancer models with minimal toxicity.
Tianli Hao, Zihan Deng, Yufei Liu, Li Liu, Deqiang Deng, Muyang Yang, Yuanyuan Geng, Yao Sun, Beilei Yue, Jonathan F. Lovell, Yushuai Liu, Lisen Lu, Honglin Jin   +12 more
wiley   +1 more source

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids. [PDF]

N Biotechnol, 2022
Jia H, Couto-Rodriguez R, Johnson S, Medina S, Novillo B, Huynh P, Kim M, Cooper C, Michalik M, Siew B, Turesson E, Maupin-Furlow JA.   +11 more
europepmc   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Site-Directed Mutagenesis Mediated by Molecular Modeling and Docking and Its Effect on the Protein-Protein Interactions of the bHLH Transcription Factors SPATULA, HECATE1, and INDEHISCENT. [PDF]

Plants (Basel)
López-Gómez P, De La Mora-Franco D, Herrera-Ubaldo H, Díaz-Quezada C, Brieba LG, de Folter S.   +5 more
europepmc   +1 more source

Role of site-directed mutagenesis and adjuvants in the stability and potency of anthrax protective antigen. [PDF]

Saudi Pharm J, 2022
Dahhas MA, Alsenaidy MA.
europepmc   +1 more source

Kinsenoside Targets IDH1 to Restore Microglial Immune‐Metabolic Homeostasis for Alzheimer's Disease Therapy

Advanced Science, EarlyView.
Dysregulated TCA cycle contributes to Alzheimer's disease (AD) pathogenesis. Here, we show that microglial isocitrate dehydrogenase 1 (IDH1) is a critical driver. Elevated IDH1 disrupts citrate metabolism and mitochondrial function, exacerbating AD pathology.
Qianqian Li, Yajin Liao, Yan‐bo Zhao, Hongxing Wu, Tong Jin, Shuoshuo Li, Yuhan Liu, Peng Li, Songying Ouyang, Zekai Li, YuTing Xia, Qian Hua, Rui‐Yuan Pan, Zengqiang Yuan   +13 more
wiley   +1 more source

Correction: Pandey et al. Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis. Biomolecules 2023, 13, 667. [PDF]

Biomolecules
Pandey S, Raut KK, Baudin A, Djemri L, Libich DS, Ponniah K, Pascal SM.   +6 more
europepmc   +1 more source