Results 191 to 200 of about 247,239 (299)

Optimization of Signal Peptide via Site-Directed Mutagenesis for Enhanced Secretion of Heterologous Proteins in Lactococcus lactis. [PDF]

Int J Mol Sci, 2022
Alias NAR, Song AA, Alitheen NB, Rahim RA, Othman SS, In LLA.   +5 more
europepmc   +1 more source

Site-Directed Mutagenesis

International Journal of Research Studies in Biosciences, 2017
openaire   +1 more source

TRAF6 Lactylation in Glycolytic Macrophages Drives NF‐κB Signaling and M1 Polarization During Orthodontic Tooth Movement

Advanced Science, EarlyView.
Schematic diagram showing compressive stress triggers glycolytic reprogramming and lactate accumulation in macrophages. Lactate mediates TRAF6 lactylation at K171/K180 dual sites, which enhances its K63‐linked ubiquitination to activate the NF‑κB pathway and promote M1 polarization. This cascade drives orthodontic tooth movement (OTM) and alveolar bone
Xinyi He, Guodong Zhao, Yun Hu, Wenya Bai, Hao Tan, Shuai Yuan, Sihan Yang, Ye Zhu, Xiaohui Xu, Leilei Zheng   +9 more
wiley   +1 more source

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids. [PDF]

N Biotechnol, 2022
Jia H, Couto-Rodriguez R, Johnson S, Medina S, Novillo B, Huynh P, Kim M, Cooper C, Michalik M, Siew B, Turesson E, Maupin-Furlow JA.   +11 more
europepmc   +1 more source

A Pan‐Methylome Framework for Population‐Scale Bacterial Epigenomics

Advanced Science, EarlyView.
A scalable quantitative framework unlocks population‐level comparative epigenomics in bacteria. By transforming site‐level data into standardized traits, this approach reconstructs methylation‐informed phylogenies and defines the core epigenome.
Bin Ma, Huimin Gong, Ran Zhuo, Zaixiao Rao, Yanxi Wan, Mingyang Wang, Zahra Zeinalzadeh, Yuan Gao, Aohan Guan, Xinrun Li, Wanting Chen, Xiliang Wang, Yun Wan, Sihua Zhang, Yuncai Xiao, Qi Huang, Paul R. Langford, Xiangru Wang, Rui Zhou, Rui Luo, Hongbo Zhou, Chengliang Zhu, Hui Jin   +22 more
wiley   +1 more source

Site-Directed Mutagenesis Mediated by Molecular Modeling and Docking and Its Effect on the Protein-Protein Interactions of the bHLH Transcription Factors SPATULA, HECATE1, and INDEHISCENT. [PDF]

Plants (Basel)
López-Gómez P, De La Mora-Franco D, Herrera-Ubaldo H, Díaz-Quezada C, Brieba LG, de Folter S.   +5 more
europepmc   +1 more source

Histone H3K18 Lactylation Promotes the Malignant Progression of Wilms Tumor via a PSRC1/AKT/HIF‐1α Positive Feedback Loop

Advanced Science, EarlyView.
In nephroblastoma, aberrant glycolysis drives lactate accumulation, which elevates histone H3K18 lactylation via p300. Lactylation of the PSRC1 promoter activates its transcription. PSRC1 competitively binds AKT, relieving PTEN‐mediated inhibition and triggering AKT/mTOR/HIF‐1α signaling.
Yanping Wang, Hongjie Gao, Bifei Zhang, Xuetian Li, Ding Li, Aihua Cao, Fengyin Sun   +6 more
wiley   +1 more source

Correction: Pandey et al. Enhancing the Conformational Stability of the cl-Par-4 Tumor Suppressor via Site-Directed Mutagenesis. Biomolecules 2023, 13, 667. [PDF]

Biomolecules
Pandey S, Raut KK, Baudin A, Djemri L, Libich DS, Ponniah K, Pascal SM.   +6 more
europepmc   +1 more source

PEAR1 Promotes Glucose Metabolism Reprogramming in Sepsis‐Associated Acute Lung Injury via AARS1‐Mediated HIF‐1α Lactylation

Advanced Science, EarlyView.
This study revealed that a PEAR1/HIF‐1α/ glycolysis/lactate/H3K18la positive feedback loop in PMVECs that drives the development of S‐ALI. Mechanistically, PEAR1 mediates the binding of HIF‐1α to AARS1, leading to the lactylation of HIF‐1α, the primary lactylation site of which is K172.
Shuai Li, Zongqing Lu, Pu Fang, Qingqing Pang, Xianghui Wu, Bo Wang, Min Liang, Jingjing Pan, Haobo Kong, Haijian Cai, Juanjuan Li, Jiaying Kang, Dongsheng Wang, Dunling Xia, Mengmeng Xu, Qinghai You   +15 more
wiley   +1 more source

Site-Directed Mutagenesis [PDF]

, 2008
openaire   +2 more sources