Results 31 to 40 of about 1,059,795 (177)

Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons [PDF]

Scientific Reports
Mobile integrons are the most efficient mechanism of Gram-negative bacteria to resist antimicrobial changing pressures in the nosocomial niche. Integron’s integrases mediate site-specific recombination of distinct DNA structures such as attI, attC and ...
Adrián Gonzales Machuca, María Carolina Molina, Verónica Elizabeth Álvarez, Eduardo José Carpio Díaz, María Paula Quiroga, Daniela Centrón   +5 more
doaj   +2 more sources

Xer Site Specific Recombination: Double and Single Recombinase Systems. [PDF]

Front Microbiol, 2017
The separation and segregation of newly replicated bacterial chromosomes can be constrained by the formation of circular chromosome dimers caused by crossing over during homologous recombination events.
Castillo F, Benmohamed A, Szatmari G.
europepmc   +2 more sources

The ins and outs of serine integrase site-specific recombination. [PDF]

Curr Opin Struct Biol, 2014
Rutherford K, Van Duyne GD.
europepmc   +2 more sources

DNA cloning using in vitro site-specific recombination.

Genome Research, 2000
As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function.
J. Hartley, G. Temple, M. Brasch
semanticscholar   +1 more source

Gated rotation mechanism of site-specific recombination by ϕC31 integrase. [PDF]

Proc Natl Acad Sci U S A, 2012
Olorunniji FJ, Buck DE, Colloms SD, McEwan AR, Smith MC, Stark WM, Rosser SJ.   +6 more
europepmc   +2 more sources

Genome engineering and direct cloning of antibiotic gene clusters via phage ϕBT1 integrase-mediated site-specific recombination in Streptomyces

Scientific Reports, 2015
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study,
Deyao Du, Lu Wang, Yuqing Tian, Hao Liu, H. Tan, G. Niu   +5 more
semanticscholar   +1 more source

Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases

Virulence, 2020
The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang, Getong Liu, Xuhui Zhang, Yaowen Chang, Shasha Wang, Weizhi He, Wenxia Sun, Dongrong Chen, Alastair I.H. Murchie   +8 more
doaj   +1 more source

Site-specific recombination strategies for engineering actinomycete genomes. [PDF]

Appl Environ Microbiol, 2012
Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, Erb A, Leadlay PF, Bechthold A, Luzhetskyy A.   +9 more
europepmc   +2 more sources

Site-specific recombination

, 2007
Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of ...
Grainge, I, Sherratt, D
openaire   +2 more sources

Site-specific recombination in plane view [PDF]

Structure, 1997
Biochemists have worked long and hard on each reaction component and chemical step to reach the point of asking the question as to how protein and DNA molecules are arranged and rearranged in the process of site-specific recombination. The structures of several lambda integrase family members published recently have answered many of the questions about
Yang, Wei, Mizuuchi, Kiyoshi
openaire   +2 more sources