Results 41 to 50 of about 1,059,795 (177)
Characterization of the integration and modular excision of the integrative conjugative element PAISt in Streptomyces turgidiscabies Car8. [PDF]
PLoS ONE, 2014 PAISt is a large genomic island located in the chromosome of the plant pathogen Streptomyces turgidiscabies Car8. The island carries clustered virulence genes, transfers to other Streptomyces species, and integrates by site-specific recombination at the ...
Jose C Huguet-Tapia +2 more
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Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]
PLoS Biology, 2006 Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh +2 more
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Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination
Nucleic Acids Research, 2013 Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
S. Colloms +7 more
semanticscholar +1 more source
In vivo site-specific recombination using the β-rec/six system
BioTechniques, 2008 The prokaryotic β serine recombinase (β-rec) catalyzes site-specific recombination between two directly oriented six sites (93 bp) in mammalian cells, both in episomal and in chromosomally integrated substrates.
Pilar Servert +9 more
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Ligand-regulated site-specific recombination. [PDF]
Proceedings of the National Academy of Sciences, 1995 Site-specific recombination offers a potential way to alter a living genome by design in a precise and stable manner. This potential requires strategies which can be used to regulate the recombination event. We describe a strategy to regulate FLP recombinase activity which relies on expressing FLP as a fusion protein with steroid hormone receptor ...
C, Logie, A F, Stewart
openaire +2 more sources
Positive-negative-selection-mediated gene targeting in rice
Frontiers in Plant Science, 2015 Gene targeting (GT) refers to the designed modification of genomic sequence(s) through homologous recombination (HR). GT is a powerful tool both for the study of gene function and for molecular breeding.
Zenpei eShimatani +4 more
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Cis- and trans-acting elements regulate the mouse Psmb9 meiotic recombination hotspot. [PDF]
PLoS Genetics, 2007 In most eukaryotes, the prophase of the first meiotic division is characterized by a high level of homologous recombination between homologous chromosomes.
Frédéric Baudat, Bernard de Massy
doaj +1 more source
eLife, 2016 Survival of Trypanosoma brucei depends upon switches in its protective Variant Surface Glycoprotein (VSG) coat by antigenic variation. VSG switching occurs by frequent homologous recombination, which is thought to require locus-specific initiation. Here,
Rebecca Devlin +8 more
doaj +1 more source
Cell-Specific Ecdysone-Inducible Expression of FLP Recombinase in Mammalian Cells
BioTechniques, 1998 The ability of site-specific recombinases, like FLP and Cre, to catalyze alterations in genomic DNA is well established, whereas their application to genetic engineering strategies has been restricted because of the inability to temporally regulate their
Janet A. Sawicki +2 more
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Transposon-specified site-specific recombination. [PDF]
Proceedings of the National Academy of Sciences, 1982 Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein ...
P, Kitts +4 more
openaire +2 more sources