Results 61 to 70 of about 1,059,795 (177)
BMC Biotechnology, 2010 Background Cre-mediated site-specific integrative recombination in mouse embryonic stem (ES) cells is a useful tool for genome engineering, allowing precise and repeated site-specific integration. To promote the integrative reaction, a left element/right
Araki Masatake +3 more
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eLife, 2016 Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Aleksandra Bebel +4 more
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Antibiotics, 2020 Modules composed of a resistance gene flanked by Xer site-specific recombination sites, the vast majority of which were found in Acinetobacter baumannii, are thought to behave as elements that facilitate horizontal dissemination. The A.
David L. Lin +5 more
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VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering
Nucleic Acids Research, 2011 We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre ...
E. Suzuki, M. Nakayama
semanticscholar +1 more source
Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
Scientific Reports, 2011 We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase ...
Lin Zhang, Guoping Zhao, Xiaoming Ding
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Structure and mechanism in site-specific recombination
Current Opinion in Structural Biology, 1999 Three-dimensional structural information on the integrase family of site-specific recombinases has only recently become available, with the crystal structures of catalytic domains, full-length proteins and protein-DNA complexes of this family reported over the past two years.
D N, Gopaul, G D, Duyne
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ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
mBio, 2018 Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates.
Kenan C. Murphy +5 more
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Ectopic Expression of O Antigen in
mSphere, 2018 We describe a novel genome integration system that enables the introduction of DNA fragments as large as 50 kbp into the chromosomes of recipient bacteria.
Keisuke Ishigaki +5 more
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Role of Xer site-specific recombination in the genesis of pJHCMW1: an evolutionary hypothesis. [PDF]
J Glob Antimicrob Resist, 2023 Traglia G, Ramirez MS, Tolmasky ME.
europepmc +1 more source
Construction and application of a multifunctional CHO cell platform utilizing Cre/lox and Dre/rox site-specific recombination systems. [PDF]
Front Bioeng Biotechnol, 2023 Zhang C +7 more
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