Hyperfusogenic Mutations Destabilize the Postfusion Six-Helix Bundle of the Measles Virus Fusion Glycoprotein. [PDF]
BiochemistryAbstract Fusion of the host membrane and viral envelope by class I viral fusion proteins is driven by the assembly of a postfusion six-helix bundle formed through antiparallel interactions between N-terminal (HR1) and C-terminal (HR2) heptad-repeat regions.
Vithanage N, Outlaw VK.
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Peptide-based Fusion Inhibitors for Preventing the Six-helix Bundle Formation of Class I Fusion Proteins: HIV and Beyond. [PDF]
Curr HIV Res, 2021 A number of different viral families have developed convergent methods to infect cells. Class I fusion proteins are commonly used by members of Arenaviridae, Coronaviridae, Filovirdae, Orthomyxoviridae, Paramyxoviridae, and Retroviridae. Class I viral fusion proteins are trimers that are involved in recognizing the cellular receptor, with a region ...
Monteiro A, Yu KOA, Hicar MD.
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Efficient replication of a paramyxovirus independent of full zippering of the fusion protein six-helix bundle domain. [PDF]
Proc Natl Acad Sci U S A, 2014 Significance Protein-mediated membrane fusion is essential for eukaryotic cell biology. In several major human viral pathogens the full closure of a six-helix bundle (6HB) fusion core structure is considered imperative for the opening of a productive fusion pore.
Brindley MA, Plattet P, Plemper RK.
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The C34 Peptide Fusion Inhibitor Binds to the Six-Helix Bundle Core Domain of HIV-1 gp41 by Displacement of the C-Terminal Helical Repeat Region. [PDF]
Biochemistry, 2015 The conformational transition of the core domain of HIV-1 gp41 from a prehairpin intermediate to a six-helix bundle is responsible for virus–cell fusion.
Louis JM, Baber JL, Clore GM.
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Stabilizing the native trimer of HIV-1 Env by destabilizing the heterodimeric interface of the gp41 postfusion six-helix bundle. [PDF]
J Virol, 2014 ABSTRACT The HIV-1 envelope glycoprotein (Env) is a trimer of gp120-gp41 heterodimers and is essential for viral entry. The gp41 subunit in native, prefusion trimeric Env exists in a metastable conformation and attains a stable six-helix bundle (6-HB) conformation comprised of a trimer of N-heptad repeat (NHR) and C-heptad repeat (CHR ...
Kesavardhana S, Varadarajan R.
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Modulating immunogenic properties of HIV-1 gp41 membrane-proximal external region by destabilizing six-helix bundle structure. [PDF]
Virology, 2016 The C-terminal alpha-helix of gp41 membrane-proximal external region (MPER; (671)NWFDITNWLWYIK(683)) encompassing 4E10/10E8 epitopes is an attractive target for HIV-1 vaccine development. We previously reported that gp41-HR1-54Q, a trimeric protein comprised of the MPER in the context of a stable six-helix bundle (6HB), induced strong immune responses ...
Banerjee S +4 more
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Inhibition of Viral Membrane Fusion by Peptides and Approaches to Peptide Design
Pathogens, 2021 Fusion of lipid-enveloped viruses with the cellular plasma membrane or the endosome membrane is mediated by viral envelope proteins that undergo large conformational changes following binding to receptors.
Nejat Düzgüneş +2 more
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Small, 2022 AbstractThe internal design of DNA nanostructures defines how they behave in different environmental conditions, such as endonuclease‐rich or low‐Mg2+ solutions. Notably, the inter‐helical crossovers that form the core of such DNA objects have a major impact on their mechanical properties and stability.
Xin, Yang +11 more
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Resistance to N-peptide fusion inhibitors correlates with thermodynamic stability of the gp41 six-helix bundle but not HIV entry kinetics. [PDF]
Retrovirology, 2014 The HIV-1 envelope glycoprotein (Env) undergoes conformational changes that mediate fusion between virus and host cell membranes. These changes involve transient exposure of two heptad-repeat domains (HR1 and HR2) in the gp41 subunit and their subsequent self-assembly into a six-helix bundle (6HB) that drives fusion.
De Feo CJ +5 more
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Identification of a Novel Inhibitor against Middle East Respiratory Syndrome Coronavirus
Viruses, 2017 The Middle East respiratory syndrome coronavirus (MERS-CoV) was first isolated in 2012, and circulated worldwide with high mortality. The continual outbreaks of MERS-CoV highlight the importance of developing antiviral therapeutics.
Yaping Sun +4 more
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