Results 91 to 100 of about 29,268 (294)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Experimental study on the tribo-chemical smoothening process between self-mated silicon carbide in a water-lubricated surface-contact reciprocating test

Friction, 2018
Silicon carbide (SiC) can be tribo-chemically smoothened during a self-mated sliding procedure in the aqueous environment. As well reported in the point-contact tests, this smoothening process works well due to the abundant water as oxidant.
Le Jin, Herbert Scheerer, Georg Andersohn, Matthias Oechsner, Dieter Hellmann   +4 more
doaj   +1 more source

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

bioRxiv, 2018
Transduction of hedgehog signals across the plasma membrane is a key process during animal development. This is facilitated by the Class F G-protein-coupled-receptor (GPCR) Smoothened (SMO), a major drug target in the treatment of basal cell carcinomas ...
G. Hedger, Heidi Koldsø, M. Chavent, C. Siebold, R. Rohatgi, M. Sansom   +5 more
semanticscholar   +1 more source

Roughing Up Smoothened: Chemical Modulators of Hedgehog Signaling [PDF]

, 2013
Small-molecule antagonists of Hedgehog-pathway signaling, such as cyclopamine, have been known for some time. Now, small-molecule agonists of the Hedgehog pathway have also been identified.
King, Randall
core  

Hedgehog Signaling: A Smoothened Conformational Switch

, 2008
SummaryAn intracellular conformational switch in the serpentine transmembrane protein Smoothened appears to underlie Hedgehog pathway activation.
Kalderon, Daniel
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

eLife, 2016
Cholesterol is necessary for the function of many G-protein coupled receptors (GPCRs). We find that cholesterol is not just necessary but also sufficient to activate signaling by the Hedgehog (Hh) pathway, a prominent cell-cell communication system in ...
G. Luchetti, R. Sircar, Jennifer H. Kong, S. Nachtergaele, A. Sagner, E. Byrne, D. Covey, C. Siebold, R. Rohatgi   +8 more
semanticscholar   +1 more source

A frequent activated smoothened mutation in sporadic basal cell carcinomas

, 1999
Basal-cell carcinomas (BCCs) are the most common cancer in Caucasians. It has been reported that the patched gene is inactivated in 30-40% sporadic BCCs and 20% sporadic medulloblastomas via loss of heterozygosity and nonsense mutations.
Chan, LYS, Tong, SF, Xie, J, Lam, CW, Ng, HK, Lim, PL, To, KF, Lee, KC, Yuen, NWF, Mccormick, F   +9 more
core   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Hedgehog Induces Opposite Changes in Turnover and Subcellular Localization of Patched and Smoothened

, 2000
Secreted signaling proteins of the Hedgehog family organize spatial pattern during animal development. Two integral membrane proteins have been identified with distinct roles in Hedgehog signaling.
Neubüser, Dagmar, Denef, Natalie, Perez, Lidia, Cohen, Stephen M   +3 more
core   +1 more source