Results 101 to 110 of about 55,518 (304)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

N-Terminal Domain of Vacuolar SNARE Vam7p Promotes Trans-SNARE Complex Assembly [PDF]

, 2012
SNARE-dependent membrane fusion in eukaryotic cells requires that the heptad-repeat SNARE domains from R- and Q-SNAREs, anchored to apposed membranes, assemble into four-helix coiled-coil bundles.
Wickner, William T, Xu, Hao
core   +1 more source

In situ formation of the loop snare technique for retrieval of foreign bodies from vessels

Journal of Interventional Medicine, 2018
Purpose: To investigate the feasibility and effectiveness of in situ formation of the loop snare technique for retrieval of foreign bodies from vessels.Materials and methods: We retrospectively reviewed in situ formation of the loop snare technique for ...
Qi Wang, Chen Zhou, Jiacheng Liu, Bin Xiong, Chuansheng Zheng, Gansheng Feng   +5 more
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis

Cell Reports, 2019
Summary: Exocytosis of synaptic vesicles and dense-core vesicles requires both the Munc13 and CAPS (Ca2+-dependent activator proteins for secretion) proteins.
Hao Zhou, Ziqing Wei, Shen Wang, Deqiang Yao, Rongguang Zhang, Cong Ma   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Orchestral Snare Drum Performance: An Historical Study. [PDF]

, 1989
Today, we differentiate snare drum performance into one of two broad styles, usually referred to as orchestral and rudimental. Since all early examples of orchestral snare drum literature are rudimental or military in nature, the evolution of present-day
Gauthreaux, Guy Gregoire, II
core   +1 more source

Histological outcomes between hot and cold snare polypectomy for small colorectal polyps

The Saudi Journal of Gastroenterology, 2017
Background/Aim: To compare the complete resection rate of hot and cold snare polypectomy for small colorectal polyps. Patients and Methods: We retrospectively reviewed the medical records of 233 consecutive patients with 461 colorectal polyps up to 10 mm
Toshiki Yamamoto, Sho Suzuki, Chika Kusano, Kyoko Yakabe, Maho Iwamoto, Hisatomo Ikehara, Takuji Gotoda, Mitsuhiko Moriyama   +7 more
doaj   +1 more source

A NEW TONSIL-SNARE [PDF]

Journal of the American Medical Association, 1909
I have been using with much satisfaction for the past two years the tonsil-snare shown in the accompanying illustration. I claim nothing original for this instrument, as it is simply a modification of the Krausmueller-Peters snare with the Vedder tip, but the modification is such and the instrument is so solidly constructed that it can be used in the ...
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source