Results 31 to 40 of about 117,630 (212)

3D Culture Models with CRISPR Screens Reveal Hyperactive NRF2 as a Prerequisite for Spheroid Formation via Regulation of Proliferation and Ferroptosis.

Molecules and Cells, 2020
Cancer-associated mutations that stabilize NRF2, an oxidant defense transcription factor, are predicted to promote tumor development. Here, utilizing 3D cancer spheroid models coupled with CRISPR-Cas9 screens, we investigate the molecular pathogenesis ...
N. Takahashi, P. Cho, L. Selfors, H. J. Kuiken, R. Kaul, Takuro Fujiwara, I. Harris, Tian Zhang, S. Gygi, J. Brugge   +9 more
semanticscholar   +1 more source

A Microfluidic Multisize Spheroid Array for Multiparametric Screening of Anticancer Drugs and Blood–Brain Barrier Transport Properties

Advancement of science, 2021
Physiological‐relevant in vitro tissue models with their promise of better predictability have the potential to improve drug screening outcomes in preclinical studies.
Christoph Eilenberger, M. Rothbauer, Florian Selinger, Anna Gerhartl, C. Jordan, M. Harasek, B. Schädl, J. Grillari, J. Weghuber, W. Neuhaus, S. Küpcü, P. Ertl   +11 more
semanticscholar   +1 more source

High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool

Journal of Tissue Engineering, 2019
Spheroid culture is a widely used three-dimensional culture technology that simulates the three-dimensional structure of tumors in vivo and has been considered a good model for tumor research.
Wei Liao, Jieqing Wang, Jiecheng Xu, Fuyu You, Mingxin Pan, Xiaoping Xu, Jun Weng, Xu Han, Shao Li, Yang Li, Kangyan Liang, Qing Peng, Yi Gao   +12 more
doaj   +1 more source

Preparation of Spheroids from Primary Pig Cells in a Mid-Scale Bioreactor Retaining Their Myogenic Potential

Cells, 2022
Three-dimensional cell culture techniques mimic the in vivo cell environment more adequately than flat surfaces. Spheroids are multicellular aggregates and we aimed to produce scaffold-free spheroids of myogenic origin, called myospheres, using a mid ...
Katja Stange, Amir Keric, Andreas Friese, Monika Röntgen   +3 more
doaj   +1 more source

Engineered Cell‐Secreted Extracellular Matrix Modulates Cell Spheroid Mechanosensing and Amplifies Their Response to Inductive Cues for the Formation of Mineralized Tissues

Advanced Healthcare Materials, 2021
The clinical translation of mesenchymal stromal cell (MSC)‐based therapies remains challenging due to rapid cell death and poor control over cell behavior.
Tomas Gonzalez-Fernandez, Alejandro J. Tenorio, Augustine M. Saiz, Jr., J. K. Leach   +3 more
semanticscholar   +1 more source

Focused Ultrasound Treatment of a Spheroid In Vitro Tumour Model

Cells, 2022
Focused ultrasound (FUS) is a non-invasive technique producing a variety of biological effects by either thermal or mechanical mechanisms of ultrasound interaction with the targeted tissue.
Lisa Landgraf, Adam Kozlowski, Xinrui Zhang, Marc Fournelle, Franz-Josef Becker, Steffen Tretbar, Andreas Melzer   +6 more
doaj   +1 more source

Quantitative analysis of tumour spheroid structure

bioRxiv, 2021
Tumour spheroids are common in vitro experimental models of avascular tumour growth. Compared with traditional two-dimensional culture, tumour spheroids more closely mimic the avascular tumour microenvironment where spatial differences in nutrient ...
A. Browning, Jesse A. Sharp, Ryan J. Murphy, G. Gunasingh, Brodie A. J. Lawson, K. Burrage, N. Haass, M. Simpson   +7 more
semanticscholar   +1 more source

Intercellular Communication in Spheroids [PDF]

, 1984
This chapter has shown that the response of spheroid cells to gap junctional communication may lead to certain metabolic and cell physiological changes. It has also become apparent that the functions of the gap junctions are very complex. They may, for example, be related to the fundamental effects of cAMP and/or Ca 2+.
Dertinger, Hermann, Hülser, Dieter F.
openaire   +4 more sources

Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines

Cancer Cell International, 2021
Background Relapse and metastasis in colorectal cancer (CRC) are often attributed to cancer stem-like cells (CSCs), as small sub-population of tumor cells with ability of drug resistance. Accordingly, development of appropriate models to investigate CSCs
E. Gheytanchi, Marzieh Naseri, Feridoun Karimi-Busheri, R. Ghods   +3 more
semanticscholar   +1 more source

3D Cardiac Cell Culture: A Critical Review of Current Technologies and Applications

Frontiers in Cardiovascular Medicine, 2019
Three-dimensional (3D) cell culture is often mentioned in the context of regenerative medicine, for example, for the replacement of ischemic myocardium with tissue-engineered muscle constructs.
Christian Zuppinger
doaj   +1 more source