A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence [PDF]
, 1977 Rabbit antibodies against actin and tubulin were used in an indirect immunofluorescence study of the structure of the mitotic spindle of PtK1 cells after lysis under conditions that preserve anaphase chromosome movement. During early prophase there is no
Cande, W. Zacheus +2 more
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eLifeThe spindle assembly checkpoint (SAC) temporally regulates mitosis by preventing progression from metaphase to anaphase until all chromosomes are correctly attached to the mitotic spindle.
KC Farrell, Jennifer T Wang, Tim Stearns
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Measurement of Spindle Rigidity by using a Magnet Loader
Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2010 The static rigidity of a rotating spindle in the radial direction is investigated in this research. A magnetic loading device (magnet loader) has been developed for the measurement.
Taku YAMAZAKI +5 more
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, 2017 Spindles are self-organized microtubule-based structures that segregate chromosomes during cell division. The mass of the spindle is controlled by the balance between microtubule turnover and nucleation. The mechanisms that control the spatial regulation
Foster, Peter J. +5 more
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A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells. [PDF]
, 2014 Dynamic instability, polarity, and spatiotemporal organization are hallmarks of the microtubule cytoskeleton that allow formation of complex structures such as the eukaryotic spindle. No similar structure has been identified in prokaryotes.
Agard, David A +6 more
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Kinetochore-independent chromosome segregation driven by lateral microtubule bundles
eLife, 2015 During cell division, chromosomes attach to spindle microtubules at sites called kinetochores, and force generated at the kinetochore-microtubule interface is the main driver of chromosome movement.
Christina C Muscat +4 more
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How Does SUMO Participate in Spindle Organization?
Cells, 2019 The ubiquitin-like protein SUMO is a regulator involved in most cellular mechanisms. Recent studies have discovered new modes of function for this protein.
Ariane Abrieu, Dimitris Liakopoulos
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Effect analysis of bearing and interface dynamics on tool point FRF for chatter stability in machine tools by using a new analytical model for spindle-tool assemblies [PDF]
, 2007 Self-excited vibration of the tool, regenerative chatter, can be predicted and eliminated if the stability lobe diagram of the spindle–holder–tool assembly is known.
Budak, Erhan +4 more
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Emerging Insights into the Function of Kinesin-8 Proteins in Microtubule Length Regulation
Biomolecules, 2018 Proper regulation of microtubules (MTs) is critical for the execution of diverse cellular processes, including mitotic spindle assembly and chromosome segregation.
Sanjay Shrestha +3 more
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Chromosome movement in lysed mitotic cells is inhibited by vanadate. [PDF]
, 1978 Mitotic PtK1 cells, lysed at anaphase into a carbowax 20 M Brij 58 solution, continue to move chromosomes toward the spindle poles and to move the spindle poles apart at 50% in vivo rates for 10 min.
CANDE, W. Zacheus, Wolniak, S
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