Cdk1 inactivation terminates mitotic checkpoint surveillance and stabilizes kinetochore attachments in anaphase [PDF]
, 2014 Two mechanisms safeguard the bipolar attachment of chromosomes in mitosis. A correction mechanism destabilizes erroneous attachments that do not generate tension across sister kinetochores [1].
Vázquez-Novelle, María Dolores +6 more
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Human TPX2 is required for targeting Aurora-A kinase to the spindle [PDF]
, 2002 Aurora-A is a serine-threonine kinase implicated in the assembly and maintenance of the mitotic spindle. Here we show that human Aurora-A binds to TPX2, a prominent component of the spindle apparatus.
Roman Körner +18 more
core +1 more source
Kinetic framework of spindle assembly checkpoint signalling [PDF]
Nature Cell Biology, 2013 The mitotic spindle assembly checkpoint (SAC) delays anaphase onset until all chromosomes have attached to both spindle poles. Here, we investigated SAC signalling kinetics in response to acute detachment of individual chromosomes using laser microsurgery. Most detached chromosomes delayed anaphase until they had realigned to the metaphase plate.
Dick AE, Gerlich DW
openaire +3 more sources
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly [PDF]
, 2009 Mitotic spindle formation and chromosome segregation depend critically on kinetochore-microtubule (KT-MT) interactions. A new protein, termed Spindly in Drosophila and SPDL-1 in C.
Erich A. Nigg +15 more
core +1 more source
Phosphorylation of Sli15 by Ipl1 is important for proper CPC localization and chromosome stability in Saccharomyces cerevisiae [PDF]
, 2014 The chromosomal passenger complex (CPC) is a key regulator of eukaryotic cell division, consisting of the protein kinase Aurora B/Ipl1 in association with its activator (INCENP/Sli15) and two additional proteins (Survivin/Bir1 and Borealin/Nbl1). Here we
David A Robinson +17 more
core +1 more source
Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay
Nature Communications, 2017 The spindle assembly checkpoint delays mitotic progression until sister chromatids are bi-oriented. Here the authors show that moderate delays in mitotic progression induce centrosome fragmentation and centriole disengagement and that spindle bipolarity ...
Menuka Karki +2 more
doaj +1 more source
Frontiers in Plant Science, 2016 The classic maize mutant divergent spindle-1 (dv1) causes failures in meiotic spindle assembly and a decrease in pollen viability. By analyzing two independent dv1 alleles we demonstrate that this phenotype is caused by mutations in a member of the ...
David M Higgins +3 more
doaj +1 more source
Regulation of chromosome congression : focus on the function of hSpindly and the kinetochore recruitment of the Ska complex [PDF]
, 2011 Mitosis is a process in which cells divide their genetic materials equally into two daughter cells. During mitosis, specialized structures called kinetochores (KTs), located at the centromeric regions of chromosomes, are captured by microtubules (MTs ...
Chan, Ying Wai
core +1 more source
Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase
Cell Reports, 2016 Summary: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown.
Michael Bokros +4 more
doaj +1 more source
Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A [PDF]
, 2013 Anaphase central spindle formation is controlled by the microtubule-stabilizing factor PRC1 and the kinesin KIF4A. We show that an MKlp2-dependent pool of Aurora B at the central spindle, rather than global Aurora B activity, regulates KIF4A accumulation
Erich A. Nigg +16 more
core +1 more source