Coordinated Spindle Assembly and Orientation Requires Clb5p-Dependent Kinase in Budding Yeast [PDF]
, 2000 The orientation of the mitotic spindle along a polarity axis is critical in asymmetric cell divisions. In the budding yeast, Saccharomyces cerevisiae, loss of the S-phase B-type cyclin Clb5p under conditions of limited cyclin-dependent kinase activity ...
Bloom, Kerry +5 more
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TheSaccharomyces cerevisiaeSpindle Pole Body Is a Dynamic Structure [PDF]
Molecular Biology of the Cell, 2003 During spindle pole body (SPB) duplication, the new SPB is assembled at a distinct site adjacent to the old SPB. Using quantitative fluorescence methods, we studied the assembly and dynamics of the core structural SPB component Spc110p. The SPB core exhibits both exchange and growth in a cell cycle-dependent manner.
Kerry Bloom +3 more
openaire +3 more sources
Illuminating spindle convex bodies and minimizing the volume of spherical sets of constant width [PDF]
Discrete and Computational Geometry 47/2 (2012), 275-287, 2011 A subset of the d-dimensional Euclidean space having nonempty interior is called a spindle convex body if it is the intersection of (finitely or infinitely many) congruent d-dimensional closed balls. The spindle convex body is called a "fat" one, if it contains the centers of its generating balls.
arxiv +1 more source
Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle [PDF]
, 2011 Insertion into and release of the cytoplasmic domain of the Schizosaccharomyces pombe spindle pole body from a nuclear envelope fenestra during mitosis requires ...
Adams +75 more
core +2 more sources
The spindle pole body component Spc97p interacts with the gamma -tubulin of Saccharomyces cerevisiae and functions in microtubule organization and spindle pole body duplication [PDF]
The EMBO Journal, 1997 Previously, we have shown that the gamma-tubulin Tub4p and the spindle pole body component Spc98p are involved in microtubule organization by the yeast microtubule organizing centre, the spindle pole body (SPB). In this paper we report the identification of SPC97 encoding an essential SPB component that is in association with the SPB substructures that
Knop, M +4 more
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Meiotic Nuclear Oscillations Are Necessary to Avoid Excessive Chromosome Associations
Cell Reports, 2016 Pairing of homologous chromosomes is a crucial step in meiosis, which in fission yeast depends on nuclear oscillations. However, how nuclear oscillations help pairing is unknown.
Mariola R. Chacón +2 more
doaj +1 more source
Toward a systems-level view of mitotic checkpoints [PDF]
Prog Biophys Mol Biol. 2015 Mar;117(2-3):217-24, 2016 Reproduction and natural selection are the key elements of life. In order to reproduce, the genetic material must be doubled, separated and placed into two new daughter cells, each containing a complete set of chromosomes and organelles. In mitosis, transition from one process to the next is guided by intricate surveillance mechanisms, known as the ...
arxiv +1 more source
Two spatially distinct kinesin-14 proteins, Pkl1 and Klp2, generate collaborative inward forces against kinesin-5 Cut7 in S. pombe [PDF]
, 2018 Kinesin motors play central roles in bipolar spindle assembly. In many eukaryotes, spindle pole separation is driven by kinesin-5, which generates outward force.
Toda, Takashi +3 more
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Synthetic Physical Interactions with the Yeast Centrosome
G3: Genes, Genomes, Genetics, 2019 The yeast centrosome or Spindle Pole Body (SPB) is an organelle situated in the nuclear membrane, where it nucleates spindle microtubules and acts as a signaling hub.
Rowan S. M. Howell +2 more
doaj +1 more source
A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast [PDF]
, 2017 Accurate chromosome segregation relies on the bipolar mitotic spindle. In many eukaryotes, spindle formation is driven by the plus-end–directed motor kinesin-5 that generates outward force to establish spindle bipolarity.
Kawakami, Tomoki +5 more
core +1 more source