Results 71 to 80 of about 34,718 (253)

Control of 3′ splice site selection by the yeast splicing factor Fyv6

eLife
Pre-mRNA splicing is catalyzed in two steps: 5ʹ splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (first and second step factors).
Katherine A Senn, Karli A Lipinski, Natalie J Zeps, Amory F Griffin, Max E Wilkinson, Aaron A Hoskins   +5 more
doaj   +1 more source

HNRNPU K181 Lactylation Drives Cervical Cancer Growth by Upregulating PHGDH and Reprogramming Serine Metabolism

Advanced Science, EarlyView.
Lactate in cervical cancer induces HNRNPU K181 lactylation, opposed by NAA50‐mediated acetylation and suppressed by Pazopanib. This lactylation enhances HNRNPU binding to PHGDH pre‐mRNA exon 1, maintaining exon 1‐containing transcripts and mRNA stability, thereby activating serine metabolism.
Chang Zhang, Qingfei Meng, Hui Jiao, Huixin Liu, Xiangchao Wang, Honglan Zhou, Yishu Wang   +6 more
wiley   +1 more source

Structural characterization of human spliceosome activation by cryo-EM [PDF]

, 2021
Eukaryotic genes are transcribed as precursor mRNA (pre-mRNA), in which coding regions (exons) are interrupted by non-coding regions (introns). Introns are excised and exons are ligated together in a two-step process termed "splicing" to produce mature
Townsend, Cole
core   +1 more source

USP15 Drives NSCLC Progression and Chemoresistance, Potentially via Regulation of the U2‐Type Spliceosomal Complex

Cancer Medicine
Objective Non‐small cell lung cancer (NSCLC) is an aggressive and lethal malignancy with the highest cancer‐related mortality rate. More than 50% of patients are diagnosed at advanced stages, often accompanied by chemoresistance and poor prognosis ...
Chien‐Chih Chiu, Sheng‐Kai Hsu, Wangta Liu, I‐Ling Lin, Wenhua Qiu, Ching‐Hung Hsieh, Chon‐Kit Chou   +6 more
doaj   +1 more source

SF3B1 mutations in myelodysplastic syndromes: A potential therapeutic target for modulating the entire disease process

Frontiers in Oncology, 2023
Myelodysplastic syndromes (MDS) are clonal hematologic malignancies characterized by ineffective hematopoiesis and dysplasia of the myeloid cell lineage and are characterized by peripheral blood cytopenia and an increased risk of transformation to acute ...
Moqin Jiang, Moqin Jiang, Moqin Jiang, Meng Chen, Meng Chen, Qian Liu, Qian Liu, Zhiling Jin, Zhiling Jin, Zhiling Jin, Xiangdong Yang, Xiangdong Yang, Weifeng Zhang, Weifeng Zhang   +13 more
doaj   +1 more source

NUDT21 Drives T‐Cell Acute Lymphoblastic Leukemia Through Dual Regulation of Alternative Polyadenylation and Transcriptional Activation

Advanced Science, EarlyView.
In summary, our study defines a coordinated oncogenic model in which NUDT21 integrates alternative polyadenylation–dependent UBE2D3 stabilization and transcriptional activation to sustain MYC‐driven T‐ALL cell survival, thereby establishing NUDT21 as a central regulatory node and a promising therapeutic target.
Conglian Qiu, Jianwei Wang, Zhiheng Li, Qi Ji, Hui Zhang, Qinyi Zhang, Senlin Zhang, Juanjuan Yu, Yanfang Tao, Yijun Wu, Chunxia Shi, Zong Zai, Zimu Zhang, Yizhen Li, Zhenjiang Bai, Shaoyan Hu, Jian Pan, Yang Yang, Shuiyan Wu   +18 more
wiley   +1 more source

Structural basis for dual roles of Aar2p in U5 snRNP assembly

, 2013
Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase.
Jovin, S.M., Beggs, J., Wahl, M., Cristao, V., Heroven, A., Cristão, V.F., Beggs, J.D., Santos, K., Santos, K.F., Holton, N., Weber, G., Mozaffari-Jovin, S., Heroven, A.C., Wahl, M.C., Lührmann, R.   +14 more
core   +1 more source

Unleashing the potential of catalytic RNAs to combat mis-spliced transcripts

Frontiers in Bioengineering and Biotechnology, 2023
Human transcriptome can undergo RNA mis-splicing due to spliceopathies contributing to the increasing number of genetic diseases including muscular dystrophy (MD), Alzheimer disease (AD), Huntington disease (HD), myelodysplastic syndromes (MDS).
Bashayer A. Khalifah, Bashayer A. Khalifah, Shareefa A. Alghamdi, Ali H. Alhasan, Ali H. Alhasan   +4 more
doaj   +1 more source

Disruption of the SNRPF–DDX24–E2F4 Feedback Loop Uncouples Splicing and Transcriptional Regulation to Suppress Ovarian Cancer Progression

Advanced Science, EarlyView.
This study identifies SNRPF as a critical oncogenic driver in ovarian cancer. By regulating a self‐sustaining SNRPF–DDX24–E2F4 feedback loop through intron retention and nonsense‐mediated decay, SNRPF couples RNA splicing with transcriptional regulation to promote tumor progression.
Yingwei Li, Zhongshao Chen, Qianqian Gao, Yuehan Gao, Ning Yang   +4 more
wiley   +1 more source

The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis

Frontiers in Immunology
The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon, Almudena Hurtado-Pico, Carlos Plaza-Sirvent, Marc Schuster, Marco Preußner, Benno Kuropka, Fan Liu, Andor Zenon Amandus Kirsten, Xiao Jakob Schmitt, Benjamin König, Miguel Álvaro-Benito, Miguel Álvaro-Benito, Esam T. Abualrous, Esam T. Abualrous, Esam T. Abualrous, Gesa I. Albert, Stefanie Kliche, Florian Heyd, Ingo Schmitz, Christian Freund   +19 more
doaj   +1 more source