Results 81 to 90 of about 37,352 (250)

Structures of the human pre-catalytic spliceosome and its precursor spliceosome [PDF]

Cell Research, 2018
The pre-catalytic spliceosome (B complex) is preceded by its precursor spliceosome (pre-B complex) and followed by the activated spliceosome (Bact complex). The pre-B-to-B and B-to-Bact transitions are driven by the ATPase/helicases Prp28 and Brr2, respectively. In this study, we report the cryo-electron microscopy structures of the human pre-B complex
Xiechao Zhan, Chuangye Yan, Xiaofeng Zhang, Jianlin Lei, Yigong Shi   +4 more
openaire   +2 more sources

Dynamics and consequences of spliceosome E complex formation

eLife, 2017
The spliceosome must identify the correct splice sites (SS) and branchsite (BS) used during splicing. E complex is the earliest spliceosome precursor in which the 5' SS and BS are defined.
Joshua Donald Larson, Aaron A Hoskins
doaj   +1 more source

A Real‐Data‐Driven Framework for Evaluating Differential Transcript Usage Methods Across Long‐Read Bulk, Single‐Cell, and Spatial Transcriptomics

Advanced Science, EarlyView.
Grounded in real biological RNA‐seq data, the framework benchmarks ten differential transcript usage (DTU) methods across short‐read bulk, long‐read bulk, long‐read single‐cell, and long‐read spatial transcriptomics. By integrating reference transcript sets with ranked DTU results, the Transcript Set Enrichment Score (TSES) identifies data‐type ...
Chenxing Zhang, Jun Liu, Qi Zhao, Huilong Yin, Angang Yang, Minhua Zheng, Rui Zhang   +6 more
wiley   +1 more source

Identification of novel interacting partners of the pre-mRNA processing factor 31 [PDF]

, 2010
Mutations in PRPF31 (RP11 locus) cause autosomal dominant retinitis pigmentosa (adRP), an inherited disorder of the retina characterised by degeneration of rod photoreceptors. PRPF31 is ubiquitously expressed and encodes a splicing factor involved in the
Fiocco, F.
core  

The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis

Frontiers in Immunology
The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon, Almudena Hurtado-Pico, Carlos Plaza-Sirvent, Marc Schuster, Marco Preußner, Benno Kuropka, Fan Liu, Andor Zenon Amandus Kirsten, Xiao Jakob Schmitt, Benjamin König, Miguel Álvaro-Benito, Miguel Álvaro-Benito, Esam T. Abualrous, Esam T. Abualrous, Esam T. Abualrous, Gesa I. Albert, Stefanie Kliche, Florian Heyd, Ingo Schmitz, Christian Freund   +19 more
doaj   +1 more source

A Metabolic‐Epigenetic Axis of AARS1/IGF2BP3‐K280 Lactylation Sustains Endometrial Cancer Stemness and Recurrence

Advanced Science, EarlyView.
Lactate‐driven endometrial cancer relapse is mediated by enzymatic protein modification. Elevated lactate levels activate AARS1, which catalyzes K280 lactylation of IGF2BP3, therefore stabilizing HMGA2 mRNA and promoting tumor stemness and recurrence.
Peng Jiang, Yunfeng Zheng, Chenfan Tian, Yuan Tu, Xiaoxia Chang, Jiaxin Yu, Hangkun Yu, Chunxia Gong, Jie Xiong, Philip N. Baker, Yong Fu, Chao Tong   +11 more
wiley   +1 more source

PTBP1‐Mediated Alternative Splicing of DNAJB6 Promotes Everolimus Resistance in Clear Cell Renal Cell Carcinoma via EIF4B/PKIB/AKT/mTOR Positive Feedback Loop

Advanced Science, EarlyView.
PTBP1‐mediated alternative splicing drives a pathogenic switch to the DNAJB6b isoform in everolimus‐resistant clear cell renal cell carcinoma. This splicing event programs a positive feedback loop including EIF4B/PKIB/AKT/mTOR, promoting aggressive therapy resistance.
Xiu‐wu Pan, Hong‐feng Zheng, Mu‐chen Li, Jia‐lin Zhou, Hang‐biao Zhang, Dong‐hao Lyu, Yi‐fan Tang, Tian‐yue Yang, Yi‐fan Liu, Zi‐chang Liu, Wen‐jie Ma, Yuan‐bo Zong, Jian‐gui Liu, Zi‐xuan Gong, Hao Zhang, Ke‐qin Dong, Wang Zhou, Jian‐qin Ye, Xin‐gang Cui   +18 more
wiley   +1 more source

The Spliceosome and Its Metal Ions

, 2011
The spliceosome is a massive complex of 5 RNAs and many proteins that associate to catalyze precursor messenger RNA splicing. The process of splicing involves two phosphoryl transfer reactions that result in intron excision and ligation of the flanking ...
Samuel E. Butcher
core   +1 more source

Microinflammation-Driven Gene Expression Dynamics in the Pathogenesis of Metabolic Disorders and Cancer

Biology
Microinflammation serves as a central mechanism linking metabolic diseases and cancer. This study integrates gene expression profiles from irritable bowel syndrome (IBS), obesity, type 2 diabetes (T2D), colorectal cancer (CRC), renal cell carcinoma (RCC),
Marian Elisa Gabrielle T. Cadungog, Lemmuel L. Tayo   +1 more
doaj   +1 more source

Cooperative Analysis of Structural Dynamics in RNA-Protein Complexes by Single-Molecule Förster Resonance Energy Transfer Spectroscopy

Molecules, 2020
RNA-protein complexes (RNPs) are essential components in a variety of cellular processes, and oftentimes exhibit complex structures and show mechanisms that are highly dynamic in conformation and structure.
Nathalie Meiser, Christin Fuks, Martin Hengesbach   +2 more
doaj   +1 more source