Results 1 to 10 of about 10,182 (283)

The 35S U5 snRNP is generated from the activated spliceosome during In vitro splicing [PDF]

PLoS ONE, 2015
Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive
Barbara Bardoni, CL Will, CL Will, DE Agafonov, EM Makarov, EM Makarov, Evgeny M. Makarov, J Deckert, JD Dignam, K Hartmuth, MC Wahl, N Behzadnia, Olga V. Makarova, OV Makarova, OV Makarova, P Fabrizio, S Bessonov, S Bessonov, SE Behrens   +18 more
core   +10 more sources

Spliceosomal snRNA Epitranscriptomics [PDF]

Frontiers in Genetics, 2021
Small nuclear RNAs (snRNAs) are critical components of the spliceosome that catalyze the splicing of pre-mRNA. snRNAs are each complexed with many proteins to form RNA-protein complexes, termed as small nuclear ribonucleoproteins (snRNPs), in the cell nucleus. snRNPs participate in pre-mRNA splicing by recognizing the critical sequence elements present
Hironori Adachi, Pedro Morais, Yi-Tao Yu
openaire   +4 more sources

RNA splicing: a split consensus reveals two major 5' splice site classes. [PDF]

Open Biol
The established consensus sequence for human 5’ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box.
Parker MT, Fica SM, Simpson GG.
europepmc   +3 more sources

Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes. [PDF]

RNA, 2023
Premessenger RNA splicing is catalyzed by the spliceosome, a multimegadalton RNA–protein complex that assembles in a highly regulated process on each intronic substrate. Most studies of splicing and spliceosomes have been carried out in human orS. cerevisiaemodel systems.
Black CS, Whelan TA, Garside EL, MacMillan AM, Fast NM, Rader SD.   +5 more
europepmc   +3 more sources

Spliceosomal immunophilins [PDF]

FEBS Letters, 2008
The spliceosome is a dynamic, macromolecular complex, which removes non‐protein‐coding introns from pre‐mRNA to form mature mRNA in a process known as splicing. This ribonucleoprotein assembly is comprised of five uridine‐rich small nuclear RNAs (snRNAs) as well as over 300 proteins.
Mesa, Annia, Somarelli, Jason A., Herrera, Rene J.   +2 more
openaire   +2 more sources

Turning on the Spliceosome [PDF]

Biophysical Journal, 2012
The spliceosome is the complex macromolecular machine responsible for removing introns from pre-mRNAs. The processes of spliceosome assembly and activation rely on the coordinated interactions of many dozens of spliceosome components to identify splice sites in a pre-mRNA, build a spliceosome, and activate the spliceosome for catalysis by formation of ...
Ivan R. Corrêa, Melissa J. Moore, Jeff Gelles, Virginia W. Cornish, Larry J. Friedman, Aaron A. Hoskins, Ming-Qun Xu   +6 more
openaire   +2 more sources

Spliceosomal intronogenesis [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Eukaryotic transcripts contain spliceosomal introns that need to be removed by pre-mRNA splicing. Although several models have been proposed to identify the mechanism of intron gain over the evolution of eukaryotes, they remain models due to a lack of experimental validation.
Scott W. Stevens, Sujin Lee
openaire   +3 more sources

Which Kind of Causal Specificity Matters Biologically? [PDF]

, 2017
Griffiths et al. (2015) have proposed a quantitative measure of causal specificity and used it to assess various attempts to single out genetic causes as being causally more specific than other cellular mechanisms, for example, alternative splicing ...
Weber, Marcel
core   +4 more sources

Spliceosome Database: a tool for tracking components of the spliceosome [PDF]

Nucleic Acids Research, 2012
The spliceosome is the extremely complex macromolecular machine responsible for pre-mRNA splicing. It assembles from five U-rich small nuclear RNAs (snRNAs) and over 200 proteins in a highly dynamic fashion. One important challenge to studying the spliceosome is simply keeping track of all these proteins, a situation further complicated by the variety ...
Cvitkovic, Ivan, Jurica, Melissa S
openaire   +5 more sources

Localized inhibition of protein phosphatase 1 by NUAK1 promotes spliceosome activity and reveals a MYC-sensitive feedback control of transcription. [PDF]

, 2020
Deregulated expression of MYC induces a dependence on the NUAK1 kinase, but the molecular mechanisms underlying this dependence have not been fully clarified.
Ade, C.P., Baluapuri, A., Chang, Y.C., Cossa, G., Eilers, M., Girard, C., Kempa, S., Kumar, A., Kuster, B., Lührmann, R., Mastrobuoni, G., Mumberg, D., Prinz, F., Roeschert, I., Schülein-Völk, C., Silveira Vidal, R., Walz, S., Wolf, E., Wortmann, L.   +18 more
core   +1 more source