Results 1 to 10 of about 13,573 (214)
Frontiers in Genetics, 2021 Many eukaryotic species contain two separate molecular machineries for removing non-coding intron sequences from pre-mRNA molecules. The majority of introns (more than 99.5% in humans) are recognized and excised by the major spliceosome, which utilizes ...
Maureen V. Akinyi, Mikko J. Frilander
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eLife, 2016 The DEAH-box NTPase Prp43 and its cofactors Ntr1 and Ntr2 form the NTR complex and are required for disassembling intron-lariat spliceosomes (ILS) and defective earlier spliceosomes.
Jean-Baptiste Fourmann +7 more
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Nuclear Pre-snRNA Export Is an Essential Quality Assurance Mechanism for Functional Spliceosomes
Cell Reports, 2019 Summary: Removal of introns from pre-mRNAs is an essential step in eukaryotic gene expression, mediated by spliceosomes that contain snRNAs as key components. Although snRNAs are transcribed in the nucleus and function in the same compartment, all except
Daniel Becker +5 more
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Human spliceosomal snRNA sequence variants generate variant spliceosomes
RNA, 2021 Human pre-mRNA splicing is primarily catalyzed by the major spliceosome, comprising five small nuclear ribonucleoprotein complexes, U1, U2, U4, U5, and U6 snRNPs, each of which contains the corresponding U-rich snRNA.
Justin W. Mabin +3 more
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Synergistic assembly of human pre-spliceosomes across introns and exons
eLife, 2018 Most human genes contain multiple introns, necessitating mechanisms to effectively define exons and ensure their proper connection by spliceosomes. Human spliceosome assembly involves both cross-intron and cross-exon interactions, but how these work ...
Joerg E Braun +3 more
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Structure of a step II catalytically activated spliceosome from Chlamydomonas reinhardtii [PDF]
The EMBO JournalPre-mRNA splicing, a fundamental step in eukaryotic gene expression, is executed by the spliceosomes. While there is extensive knowledge of the composition and structure of spliceosomes in yeasts and humans, the structural diversity of spliceosomes in ...
Yichen Lu, Ke Liang, Xiechao Zhan
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Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes
RNA, 2023 Premessenger RNA splicing is catalyzed by the spliceosome, a multimegadalton RNA–protein complex that assembles in a highly regulated process on each intronic substrate.
C. Black +5 more
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Crosstalk between chromatin state and ATM signalling in DNA damage-induced transcription stress [PDF]
The EMBO JournalThe DNA Damage Response (DDR) is a highly regulated process that safeguards genomic integrity against DNA lesions. Increasing evidence supports a reciprocal relationship between damaged chromatin architecture and the signalling pathways that coordinate ...
Irene Salas-Armenteros +3 more
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PRPF8-mediated dysregulation of hBrr2 helicase disrupts human spliceosome kinetics and 5´-splice-site selection causing tissue-specific defects [PDF]
Nature CommunicationsThe carboxy-terminus of the spliceosomal protein PRPF8, which regulates the RNA helicase Brr2, is a hotspot for mutations causing retinitis pigmentosa-type 13, with unclear role in human splicing and tissue-specificity mechanism.
Robert Atkinson +31 more
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Animals, 2022 In mammals, the testis-specific serine/threonine kinase (TSSK) is essential for spermatogenesis and male fertility. TSSK4 belongs to the family of the testis-specific serine/threonine-protein kinase (TSSK), with a crucial role in spermatogenesis.
Xingdong Wang +11 more
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