A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor [PDF]
, 1997 We have identified a human splicing factor required for the second step of pre-mRNA splicing. This new protein, hPrp18, is 30% identical to the yeast splicing factor Prp18.
Horowitz, D. S., Krainer, A. R.
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A day in the life of the spliceosome [PDF]
Nature Reviews Molecular Cell Biology, 2014 One of the most amazing findings in molecular biology was the discovery that eukaryotic genes are discontinuous, interrupted by stretches of non-coding sequence. The subsequent realization that the intervening regions are removed from pre-mRNA transcripts via the activity of a common set of small nuclear RNAs (snRNAs), which assemble together with ...
A. Gregory Matera, Zefeng Wang
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RNAtomy of the Spliceosome’s heart [PDF]
The EMBO Journal, 2013 In his 1543 monumental work De humanis corpori fabrica , Andreas Vesalius used rigorous dissection practices and a mechanistic view of the organ's function to provide the first accurate anatomical description of a human heart. Guided by similar principles of meticulous structural probing and mechanistic explanatory potential, Anokhina and colleagues in
Juan Valcárcel, Sophie Bonnal
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Structures of the human spliceosomes before and after release of the ligated exon
Cell Research, 2018 Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ligation, have been structurally characterized.
Xiaofeng Zhang +6 more
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Cloning and tissue expression of alternative spliceosome in chicken G-protein alpha subunit gene
浙江大学学报. 农业与生命科学版, 2019 In order to research deeply the transcription and splicing situations of chicken G-protein alpha subunit gene (GNAS), we used 5´ and 3´ rapid-amplification cDNA end (RACE) technology to clone and sequence the chicken GNAS gene, and used quantitative ...
ZHANG Xuedong +6 more
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Structural basis for dual roles of Aar2p in U5 snRNP assembly [PDF]
, 2013 Yeast U5 small nuclear ribonucleoprotein particle (snRNP) is assembled via a cytoplasmic precursor that contains the U5-specific Prp8 protein but lacks the U5-specific Brr2 helicase.
Beggs, J.D. +8 more
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Writing a wrong: Coupled RNA polymerase II transcription and RNA quality control [PDF]
, 2019 Processing and maturation of precursor RNA species is coupled to RNA polymerase II transcription. Co-transcriptional RNA processing helps to ensure efficient and proper capping, splicing, and 3' end processing of different RNA species to help ensure ...
Hughes, Katlyn D. +3 more
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Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
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Therapeutic Targeting of Alternative Splicing: A New Frontier in Cancer Treatment
Frontiers in Oncology, 2022 The ability for cells to harness alternative splicing enables them to diversify their proteome in order to carry out complex biological functions and adapt to external and internal stimuli. The spliceosome is the multiprotein-RNA complex charged with the
Anthony J. Murphy +3 more
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Bidirectional coupling of splicing and ATM signaling in response to transcription-blocking DNA damage [PDF]
, 2016 In response to DNA damage cells activate intricate protein networks to ensure genomic fidelity and tissue homeostasis. DNA damage response signaling pathways coordinate these networks and determine cellular fates, in part, by modulating RNA metabolism ...
Marteijn, J.A. (Jurgen) +2 more
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