Engineering splicing factors with designed specificities [PDF]
Nature Methods, 2009 Alternative splicing is generally regulated by trans-acting factors that specifically bind pre-mRNA to activate or inhibit the splicing reaction. This regulation is critical for normal gene expression, and dysregulation of splicing is closely associated with human diseases. Here we engineer artificial splicing factors by combining sequence-specific RNA-
Yang, Wang +3 more
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The lack of the Celf2a splicing factor converts a Duchenne genotype into a Becker phenotype [PDF]
, 2016 Substitutions, deletions and duplications in the dystrophin gene lead to either the severe Duchenne muscular dystrophy (DMD) or mild Becker muscular dystrophy depending on whether out-of-frame or in-frame transcripts are produced.
BOZZONI, Irene +7 more
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PLoS Genetics, 2019 Gene expression dynamics can be measured in single living cells. Using a detectable transcriptionally active gene in living cells, we previously found that an mRNA undergoing several splicing events was retained at this gene after transcription until ...
Hodaya Hochberg-Laufer +5 more
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Alternative splicing regulation during C. elegans development: splicing factors as regulated targets. [PDF]
PLoS Genetics, 2008 Alternative splicing generates protein diversity and allows for post-transcriptional gene regulation. Estimates suggest that 10% of the genes in Caenorhabditis elegans undergo alternative splicing. We constructed a splicing-sensitive microarray to detect
Sergio Barberan-Soler, Alan M Zahler
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Functional roles of protein splicing factors [PDF]
Bioscience Reports, 2012 RNA splicing is one of the fundamental processes in gene expression in eukaryotes. Splicing of pre-mRNA is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of five small nuclear RNAs and numerous protein factors. The spliceosome is a highly dynamic structure, assembled by sequential binding and release of the small ...
Chen, Hsin-Chou, Cheng, Soo-Chen
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Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites [PDF]
Nucleic Acids Research, 2023 Abstract The fidelity of splice site selection is critical for proper gene expression. In particular, proper recognition of 3′-splice site (3′SS) sequences by the spliceosome is challenging considering the low complexity of the 3′SS consensus sequence YAG. Here, we show that absence of the Prp18p splicing factor results in genome-wide
Kevin R Roy +7 more
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Regulation of splicing factors by alternative splicing and NMD is conserved between kingdoms yet evolutionarily flexible. [PDF]
, 2015 Ultraconserved elements, unusually long regions of perfect sequence identity, are found in genes encoding numerous RNA-binding proteins including arginine-serine rich (SR) splicing factors.
Brenner, Steven, Lareau, Liana
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Alternative Splicing Events and Splicing Factors Are Prognostic in Adrenocortical Carcinoma [PDF]
Frontiers in Genetics, 2020 Alternative splicing is involved in the pathogenesis of human diseases, including cancer. Here, we investigated the potential application of alternative splicing events (ASEs) and splicing factors (SFs) in the prognosis of adrenocortical carcinoma (ACC).
Jian Lv +5 more
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Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]
, 2009 International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David +6 more
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Inhibition of IRE1α-mediated XBP1 mRNA cleavage by XBP1 reveals a novel regulatory process during the unfolded protein response [PDF]
, 2017 Background: The mammalian endoplasmic reticulum (ER) continuously adapts to the cellular secretory load by the activation of an unfolded protein response (UPR).
Bulleid, Neil J. +4 more
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