Results 1 to 10 of about 30,137 (254)

Laboratory evaluation of stable isotope labeling of Culicoides (Diptera: Ceratopogonidae) for adult dispersal studies [PDF]

Parasites & Vectors, 2019
Background Stable isotope labeling is a promising method for use in insect mark-capture and dispersal studies. Culicoides biting midges, which transmit several important animal pathogens, including bluetongue virus (BTV) and epizootic hemorrhagic disease
Emily G. McDermott, Bradley A. Mullens, Christie E. Mayo, E. Brendan Roark, Christopher R. Maupin, Alec C. Gerry, Gabriel L. Hamer   +6 more
doaj   +2 more sources

Mass Spectrometric Quantification of the Antimicrobial Peptide Pep19-2.5 with Stable Isotope Labeling and Acidic Hydrolysis [PDF]

Pharmaceutics, 2021
Sepsis is the number one cause of death in intensive care units. This life-threatening condition is caused by bacterial infections and triggered by endotoxins of Gram-negative bacteria that leads to an overreaction of the immune system.
Sabrina Wohlfart, Michael Kilian, Philip Storck, Thomas Gutsmann, Klaus Brandenburg, Walter Mier   +5 more
doaj   +2 more sources

Scaling up the production of fungal perylenequinones and investigating their biosynthesis through stable isotope labeling [PDF]

Microbial Cell Factories
Background Perylenequinones, such as hypocrellins and hypomycins, are fungal secondary metabolites with potential for pharmaceutical and industrial applications due to both their physical and biological properties. This study focused on their sustainable
Reema A. Al-Qiam, Sachin Dumbare, Tyler N. Graf, Warren S. Vidar, Huzefa A. Raja, Cedric J. Pearce, Shabnam Hematian, Nicholas H. Oberlies   +7 more
doaj   +2 more sources

Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling [PDF]

Biomolecules, 2020
Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm ...
Hirokazu Yagi, Saeko Yanaka, Rina Yogo, Akari Ikeda, Masayoshi Onitsuka, Toshio Yamazaki, Tatsuya Kato, Enoch Y. Park, Jun Yokoyama, Koichi Kato   +9 more
doaj   +2 more sources

Metabolomics of Early Stage Plant Cell–Microbe Interaction Using Stable Isotope Labeling [PDF]

Frontiers in Plant Science, 2018
Metabolomics has been used in unraveling metabolites that play essential roles in plant–microbe (including pathogen) interactions. However, the problem of profiling a plant metabolome with potential contaminating metabolites from the coexisting microbes ...
Qiuying Pang, Qiuying Pang, Tong Zhang, Yang Wang, Wenwen Kong, Qijie Guan, Xiufeng Yan, Sixue Chen, Sixue Chen, Sixue Chen   +9 more
doaj   +2 more sources

Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique [PDF]

Nutrition & Metabolism, 2018
Background Iron is a kind of essential trace mineral in the human body, while the studies on its physiological requirement are very limited recently, especially in China.
Jie Cai, Tongxiang Ren, Yuhui Zhang, Zhilin Wang, Lingyan Gou, Zhengwu Huang, Jun Wang, Jianhua Piao, Xiaoguang Yang, Lichen Yang   +9 more
doaj   +2 more sources

Synthesis of Stable Isotope Deuterium Labeled Levofloxacin-D3

Journal of Isotopes, 2023
In order to improve the stable isotope utilization rate, a simple and inexpensive synthesis method was developed. The synthesis of levofloxacin is based on piperazine as the starting material, and the reaction with carbobenzoxy chloride (CBZ-Cl) to get ...
XU Zhong jie;ZHANG Li;SUN Wen;ZHANG Pengshuai;LEI Wen
doaj   +1 more source

Synthesis of Sulfachloropyridazine-Benzene Ring-13C6

Journal of Isotopes, 2023
A synthesis route for stable isotope-labeled sulfamethoxazole-benzene ring-13C6 has been developed. In this strategy, 13C6-benzene was used as the source and starting material for stable isotope labeling and starting material to synthesize ...
MEI Shu;GUO Hui;CHEN Wulian
doaj   +1 more source

15N Metabolic Labeling Quantification Workflow in Arabidopsis Using Protein Prospector

Frontiers in Plant Science, 2022
Metabolic labeling using stable isotopes is widely used for the relative quantification of proteins in proteomic studies. In plants, metabolic labeling using 15N has great potential, but the associated complexity of data analysis has limited its usage ...
Ruben Shrestha, Andres V. Reyes, Andres V. Reyes, Peter R. Baker, Zhi-Yong Wang, Robert J. Chalkley, Shou-Ling Xu, Shou-Ling Xu   +7 more
doaj   +1 more source

Stable isotopic labeling in proteomics [PDF]

PROTEOMICS, 2008
AbstractLabeling of proteins and peptides with stable heavy isotopes (deuterium, carbon‐13, nitrogen‐15, and oxygen‐18) is widely used in quantitative proteomics. These are either incorporated metabolically in cells and small organisms, or postmetabolically in proteins and peptides by chemical or enzymatic reactions.
Gevaert, Kris, Impens, Francis, Ghesquière, Bart, Van Damme, Petra, Lambrechts, Anja, Vandekerckhove, Joël   +5 more
openaire   +3 more sources