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Production of mutant streptavidin protein and investigation of its effect on the performance of streptavidin [PDF]
Objective(s): Streptavidin is a versatile protein in cell science. The tetramer structure of streptavidin plays a key role in this binding, but this form interferes with some assays.
Elham Didevara +3 more
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Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates
We recently reported on a potent synthetic agent, 135H11, that selectively targets the receptor tyrosine kinase, EphA2. While 135H11 possesses a relatively high binding affinity for the ligand-binding domain of EphA2 (Kd~130 nM), receptor activation in ...
Parima Udompholkul +6 more
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Background CARs are engineered receptors comprising an immunoglobulin single‐chain variable fragment (scFv) that identifies and binds to the target antigen, a transmembrane domain, and an intracellular T‐cell signaling domain.
Hui Lian +10 more
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Nearly 95% of streptavidin which is expressed in Escherichia coli found as an inclusion body. Protein expressed in an inclusion body form requires further steps for the folding process related to its purification.
Toto Subroto +5 more
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The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design ...
Yong Chang +4 more
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A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed.
Lyubov V. Barshevskaya +3 more
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An immunoassay is an analytical test method in which analyte quantitation is based on signal responses generated as a consequence of an antibody–antigen interaction. They are the method of choice for the measurement of a large panel of diagnostic markers.
Amy H. A. Balzer +1 more
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While label-free multiplex sensor technology enables “mixing and matching” of different capture molecules in principle, in practice this has been rarely (if ever) demonstrated. To fill this gap, we developed protocols for the preparation of mixed aptamer-
Alanna M. Klose, Benjamin L. Miller
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The exceptional strength and stability of noncovalent avidin-biotin binding is widely utilized as an effective bioconjugation strategy in various biosensing applications, and neutravidin and streptavidin proteins are two commonly used avidin analogues ...
Tun Naw Sut +4 more
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Synthesis and Application of Albumin Nanoparticles Loaded with Prussian Blue Nanozymes
Prussian blue nanozymes exhibit peroxidase-like catalytic activity and are therefore considered a stable and inexpensive alternative to natural peroxidases in the enzyme-linked immunosorbent assay (ELISA).
Pavel Khramtsov +6 more
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